Microbial paclitaxel: advances and perspectives

Flores-Bustamante, Zoila R.; Rivera-Orduña, Flor N.; Martínez-Cárdenas, Anahí; Flores-Cotera, Luis B.

doi:10.1038/ja.2010.83

Cited by 102 publications

(78 citation statements)

References 74 publications

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“…The isolated Taxomyces andreanae was subject to a patent application and deposited at the Centraalbureau voor Schimmelcultures (Utrecht, The Netherlands) as number CBS 279.92 (Strobel et al 1994). Several other groups soon confirmed the findings in this ground-breaking publication and provided additional supporting evidence (Flores-Bustamante et al 2010). Microbial Taxol and taxane biosynthesis was found in several different genera of fungi, including Alternaria, Aspergillus, Cladosporium, Fusarium, Monochaetia, Pestlotia, Pestalotiopsis, Pithomyces, Penicillium and Xylaria, which were isolated from yew and non-Taxus plants (FloresBustamante et al 2010;Strobel et al 1996;Soca-Chafre et al 2011;Zhang et al 2009;Zhao et al 2009;Hoffman 2003).…”

Section: Introductionsupporting

confidence: 53%

Getting to the bottom of Taxol biosynthesis by fungi

2013

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Taxol (paclitaxel) is a highly-oxygenated diterpenoid natural product first isolated from the pacific yew tree (Taxus brevifolia). It is one of the most widely used anticancer drugs. Soon after the discovery of its unique mode of action and the resulting high demand, an extensive search was initiated for alternative sources to replace the slow-growing and scarce pacific yew. Thus far, however, Taxol and related compounds have only been found in the genus Taxus, which comprises a small number of slow-growing plants with a broad but generally isolated geographical distribution. In 1993, Stierle and colleagues reported the independent biosynthesis of Taxol in an endophytic fungus isolated from Taxus brevifolia, which resulted in more than 160 subsequent publications and patents addressing the biosynthesis of Taxol and related taxanes by microorganisms. The literature on fungal taxane synthesis contains numerous inconsistencies, prompting us to thoroughly re-examine Taxol biosynthesis in endophytic fungi associated with Taxus spp. Using a combination of phytochemistry, molecular biology and genome sequencing, we were unable to find any evidence for independent taxane biosynthesis in any of the endophytes, including the isolate described in the original publication (Taxomyces andreanae) and several more recent isolates from Taxus trees. Our findings therefore resolve a long-standing mystery concerning the evolution of a complex terpenoid biosynthetic pathway in two distantly-related organisms.

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Section: Introductionsupporting

confidence: 53%

Getting to the bottom of Taxol biosynthesis by fungi

2013

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“…54,57 Fungi such as Taxomyces andreanae, Pestalotiopsis microspora, Tubercularia sp., Phyllosticta citricarpa, Nodulisporium sylviforme, Colletotrichum gloeosporoides, Colletotrichum annutum, Fusarium maire and Pestalotiopsis versicolor produce it. 54,[58][59][60][61][62][63][64] The endophyte F. maire produces 225 μg l − 1 .…”

Section: Antineoplastic Drugsmentioning

confidence: 99%

Production of valuable compounds by molds and yeasts

Demain

Martens

2016

J Antibiot

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where he has contributed in a major way to the reputation of this department for many years. He also served as an Adjunct Professor at the University of Geneva. Among Julian's areas of study and accomplishment are fungal toxins including α-sarcin, chemical synthesis of triterpenes, mode of action of streptomycin and other aminoglycoside antibiotics, biochemical mechanisms of antibiotic resistance in clinical isolates of bacteria harboring resistance plasmids, their origins and evolution, secondary metabolism of microorganisms, structure and function of bacterial ribosomes, antibiotic resistance mutations in yeast ribosomes, cloning of resistance genes from an antibiotic-producing microbe, gene cloning for industrial purposes, engineering of herbicide resistance in useful crops, bleomycin-resistance gene in clinical isolates of Staphylococcus aureus and many other topics. He has been an excellent teacher, lecturing in both English and French around the world, and has organized international courses. Julian has also served on the NIH study sections, as Editor for several international journals, and was one of the founders of the journal Plasmid. We expect the impact of Julian's accomplishments to continue into the future.

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“…Most studies aimed at the exploitation of endophytes on an industrial scale have focussed on taxol producing endophytes (Flores-Bustamante et al, 2010;Gond et al, 2014;Zhou et al, 2010). Recently, with the first report of a camptothecin (CPT) producing endophyte by Puri et al (2005), the CPT producing endophytes have also attracted a lot of interest with reactor studies (Amna et al, 2006;Rehman et al, 2009) as well as the application of yield enhancement strategies being explored.…”

Section: Introductionmentioning

confidence: 99%

Reprint of: Effect of fermentation parameters, elicitors and precursors on camptothecin production from the endophyte Fusarium solani

Venugopalan

Potunuru

Dixit

et al. 2016

Bioresource Technology

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Volumetric productivity of camptothecin from the suspension culture of the endophyte Fusarium solani was enhanced up to ∼152 fold (from 0.19μgl(-1)d(-1) to 28.9μgl(-1)d(-1)) under optimized fermentation conditions including initial pH (6.0), temperature (32°C) and agitation speed (80rpm) with (5% (v/v)) ethanol as medium component. Among various elicitors and precursors studied, tryptamine (0.5mM) as precursor and bovine serum albumin (BSA) (0.075mM) as an elicitor added on day 6 of the cultivation period resulted in maximum enhancement of camptothecin concentration (up to 4.5 and 3.4-fold, respectively). These leads provide immense scope for further enhancement in camptothecin productivity at bioreactor level. The cytotoxicity analysis of the crude camptothecin extract from the fungal biomass revealed its high effectiveness against colon and mammary gland cancer cell lines.

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Microbial paclitaxel: advances and perspectives

Cited by 102 publications

References 74 publications

Getting to the bottom of Taxol biosynthesis by fungi

Getting to the bottom of Taxol biosynthesis by fungi

Production of valuable compounds by molds and yeasts

Reprint of: Effect of fermentation parameters, elicitors and precursors on camptothecin production from the endophyte Fusarium solani

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