Interferon-gamma and interleukin-10 profile of children with tuberculosis in North Sumatera, Indonesia

Daulay, Rini Savitri; Daulay, Ridwan M.

doi:10.1088/1755-1315/125/1/012147

Cited by 5 publications

(5 citation statements)

References 7 publications

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“…This study only examined one polymorphism while many polymorphisms in IFN-γ genes affect the expression of this gene and this IFN-γ +874 T/A have been studied in children [24]. This study also did not examine the levels of IFN-γ which was associated with the polymorphism +874 T/A of the IFN-γ gene.…”

Section: Hwementioning

confidence: 99%

Association of Polymorphism +874 T/A Interferon Gamma Gene with Susceptibility to Pulmonary Tuberculosis in Medan, Indonesia

Kaban

Sinaga

Nasution

2021

Open Access Maced J Med Sci

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BACKGROUND: Pulmonary tuberculosis (TB) is still a disease that concerns in the world due to its high incidence, especially in developing countries, including Indonesia. Polymorphism +874 T/A interferon-gamma (IFN-γ) is one of the host genetic factors that have been widely studied and has been shown to be associated with susceptibility to pulmonary TB. AIM: This study aims to determine the association polymorphism +874 T/A IFN-γ gene with susceptibility to pulmonary TB in Medan population. METHODS: A total of 82 pulmonary TB patients and 85 healthy controls were examined in this case–control study. SPSS 25 is used to process and analyze all data where the Pearson Chi-square is used to analyze the association between genotype and susceptibility to pulmonary TB. RESULTS: The polymorphism +874 T/A IFN-γ gene was not significantly associated with susceptibility to pulmonary TB in patients compared to controls (p = 0.395) and there was no association between allele frequency and susceptibility to pulmonary TB (p = 0.158). CONCLUSION: Our study suggests no association of polymorphism +874 T/A IFN-γ gene with pulmonary TB in Medan, Sumatera Utara, Indonesia.

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Section: Hwementioning

confidence: 99%

Association of Polymorphism +874 T/A Interferon Gamma Gene with Susceptibility to Pulmonary Tuberculosis in Medan, Indonesia

Kaban

Sinaga

Nasution

2021

Open Access Maced J Med Sci

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“…The PBMCs were carried out and washed twice with PBS, then added with complete RPMI media. PBMCs in number of 5x10 5 were cultured in 24-well plates with complete RPMI media, then treated with/without 5 μg/mL b-glucan (Merck KGaA, Darmstadt, Germany) and 10 μg/mL M. tuberculosis wild-type or mutant DNA. PBMCs were incubated in a humidified incubator of 5% CO 2 at 37°C for 6 days.…”

Section: Pbmcs Isolation and Inductionmentioning

confidence: 99%

“…However, insufficient amounts due to immune cells weakening, could be happened in children. (5) To increase the production of cytokines, immune cells need to get external immunomodulators. b-glucan, a potent immunomodulator, can affect both innate and adaptive immune systems through various mechanisms.…”

Section: Introductionmentioning

confidence: 99%

beta-Glucan Increases IFN-gamma and IL-12 Production of Peripheral Blood Mononuclear Cells with/without Induction of Mycobacterium tuberculosis Wild-type/Mutant DNA

et al. 2019

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BACKGROUND: In tuberculosis infections, the immune system is weakened and cannot produce enough cytokines to against the infection. b-glucan is a potent immunomodulator that induces cytokine production in various bacterial infections. This study aimed to determine the effects of b-glucan on the production of interferon (IFN)-γ and interleukin (IL)-12 in peripheral blood mononuclear cells (PBMCs) induced by Mycobacterium tuberculosis DNA.METHODS: PBMCs were isolated from 11 healthy subjects. PBMCs were treated with/without 5 μg/mL b-glucan and M. tuberculosis rpoB wild-type or mutant DNA. The production of IFN-γ and IL-12 in the supernatant was performed with enzyme-linked immune-sorbent assay (ELISA).RESULTS: b-glucan increased significantly (p<0.05) IFN-γ of M. tuberculosis mutant DNA-induced PBMCs, M. tuberculosis wild-type DNA-induced PBMCs, and non-induced PBMCs. b-glucan also increased significantly (p<0.05) IL-12 of M. tuberculosis mutant DNA-induced PBMCs, M. tuberculosis wild-type DNA-induced PBMCs, and non-induced PBMCs. There were not any significant difference between male and female groups for IL-12 and IFN-γ in all treatment groups (p>0.05, ANOVA test).CONCLUSION: This in vitro study indicates that b-glucan increases the performance of PBMCs to produce IFN-γ and IL-12, with/without induction of M. tuberculosis wild-type/ mutant DNA.KEYWORDS: b-glucan, IFN-γ, IL-12, M. tuberculosis, rpoB

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“…Human immune response has a potential implication in the control and prevention of TB disease, thus it is so essential to comprehend its regulation; especially the cell-mediated immune response that influenced the main response of the host against M. tuberculosis, in which important role is attributed to cytokine and T helper-1 cells. 1 Many factors can affect this response, one of them is the micronutrients, copper, and zinc have a vital role in the growth, differentiation, and proliferation of cells of acquired and innate immunity. Both these nutrients play a crucial role in the resistance of the human body to certain infections as tuberculosis.…”

Section: Introductionmentioning

confidence: 99%

Effects of anti-Tuberculosis Drugs on the Level of some Nutritional Factors Among Patients with tuberculosis

Chabuck,

Alhelli,

Jasim

2021

J. Contemp. Med. Sci.

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Objectives:The study aimed to assess changes in the level of some nutritional elements including zinc and copper for tuberculosis patients before, during and after anti-tuberculosis therapy. Methods: Whole blood samples are collected from 120 tuberculosis patients who are admitted to specialist clinic of the respiratory diseases in Babylon province, within a period from November 2020 to January 2021. Results: Out of whole TB patients, (66) ones were males and the remaining (54) were females, the age of patients ranging from (13-70) years. Sixty, apparently healthy control groups who had no Tuberculosis history were chosen. Using spectrophotometer for estimating the level of Zinc and copper. In TB patients the zinc level was increased during and after anti-tuberculosis therapy, but the level of zinc lower in patients after therapy compared with controls. The level of copper was decreased during and after the course of anti-tuberculosis, but the level of copper higher in patients after therapy compared with controls. Conclusion: These parameters could be used in follow-up as the achievement of TB therapy success.

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Interferon-gamma and interleukin-10 profile of children with tuberculosis in North Sumatera, Indonesia

Cited by 5 publications

References 7 publications

Association of Polymorphism +874 T/A Interferon Gamma Gene with Susceptibility to Pulmonary Tuberculosis in Medan, Indonesia

Association of Polymorphism +874 T/A Interferon Gamma Gene with Susceptibility to Pulmonary Tuberculosis in Medan, Indonesia

beta-Glucan Increases IFN-gamma and IL-12 Production of Peripheral Blood Mononuclear Cells with/without Induction of Mycobacterium tuberculosis Wild-type/Mutant DNA

Effects of anti-Tuberculosis Drugs on the Level of some Nutritional Factors Among Patients with tuberculosis

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