Interfacing Synthetic DNA Logic Operations with Protein Outputs

Prokup, Alexander; Deiters, Alexander

doi:10.1002/anie.201406892

Cited by 39 publications

(24 citation statements)

References 39 publications

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“…Although a number of DNA logic gates have been developed for biosensing purposes, the sensitivity is always insufficient for sensitive detection of low‐abundant miRNA. To improve the analytical performance, we have introduced hybridization chain reaction in the system for signal amplification.…”

Section: Resultsmentioning

confidence: 99%

Gold Nanoparticles‐Based DNA Logic Gate for miRNA Inputs Analysis Coupling Strand Displacement Reaction and Hybridization Chain Reaction

Gao

Tang

2017

Part & Part Syst Charact

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The molecular level DNA logic gates assisted by nanomaterials hold great promise for disease diagnosis applications. However, designing convenient and sensitive logic gates for molecular diagnostics still remains challenging. In this work, a DNA logic gate platform for miRNA inputs analysis based on the observation of localized surface plasmon resonance variation of gold nanoparticles (AuNPs) is fabricated. As a demonstration, two biomarkers to differentiate indolent and aggressive forms of prostate cancer, miR‐200c and miR‐605, are selected as examples of logical inputs. In addition, five DNA probes are designed according to strand displacement reaction and hybridization chain reaction which are required for DNA logical operation. Since target miRNA inputs are able to trigger DNA structural transformations, which are further used to precisely regulate salt‐induced AuNPs aggregation, miRNA inputs information can be converted to the information of AuNPs states as outputs. This developed system performs amplified detection of low‐abundant target miRNAs without the requirement of any enzymes. In addition, single base‐pair mismatched miRNAs can be effectively differentiated. High‐order logic gates can also be developed with further modifications. Therefore, the DNA AND logic gate is successfully constructed with flexible operations, which has potential in biochemical study and disease diagnosis.

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Section: Resultsmentioning

confidence: 99%

Gold Nanoparticles‐Based DNA Logic Gate for miRNA Inputs Analysis Coupling Strand Displacement Reaction and Hybridization Chain Reaction

Gao

Tang

2017

Part & Part Syst Charact

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Section: Figmentioning

confidence: 99%

A survey of advancements in nucleic acid-based logic gates and computing for applications in biotechnology and biomedicine

Wan

Hou

et al. 2015

Chem. Commun.

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Nucleic acid-based logic devices were first introduced in 1994. Since then, science has seen the emergence of new logic systems for mimicking mathematical functions, diagnosing disease and even imitating biological systems. The unique features of nucleic acids, such as facile and high-throughput synthesis, Watson-Crick complementary base pairing, and predictable structures, together with the aid of programming design, have led to the widespread applications of nucleic acids (NA) for logic gating and computing in biotechnology and biomedicine. In this feature article, the development of in vitro NA logic systems will be discussed, as well as the expansion of such systems using various input molecules for potential cellular, or even in vivo, applications.

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“…In recent decades, av ariety of chemical or biochemical molecules, such as enzymes, antibodies, metal ions, and DNA, have been employed as buildinge lements to fabricate differentk inds of molecularl ogic platforms. [1][2][3][4][5][6][7][8][9][10] Amongw hich, DNA is considered to be ap romising alternative due to its sequence specificity, low cost, flexible design,a nd predictable structure. [11] Many DNA logic circuits have been reported to mimic basic, advanced, or even concatenatedl ogic computation.…”

Section: Introductionmentioning

confidence: 99%

Tyramine Hydrochloride Based Label‐Free System for Operating Various DNA Logic Gates and a DNA Caliper for Base Number Measurements

et al. 2017

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DNA is believed to be a promising candidate for molecular logic computation, and the fluorogenic/colorimetric substrates of G‐quadruplex DNAzyme (G4zyme) are broadly used as label‐free output reporters of DNA logic circuits. Herein, for the first time, tyramine‐HCl (a fluorogenic substrate of G4zyme) is applied to DNA logic computation and a series of label‐free DNA‐input logic gates, including elementary AND, OR, and INHIBIT logic gates, as well as a two to one encoder, are constructed. Furthermore, a DNA caliper that can measure the base number of target DNA as low as three bases is also fabricated. This DNA caliper can also perform concatenated AND–AND logic computation to fulfil the requirements of sophisticated logic computing.

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Interfacing Synthetic DNA Logic Operations with Protein Outputs

Cited by 39 publications

References 39 publications

Gold Nanoparticles‐Based DNA Logic Gate for miRNA Inputs Analysis Coupling Strand Displacement Reaction and Hybridization Chain Reaction

Gold Nanoparticles‐Based DNA Logic Gate for miRNA Inputs Analysis Coupling Strand Displacement Reaction and Hybridization Chain Reaction

A survey of advancements in nucleic acid-based logic gates and computing for applications in biotechnology and biomedicine

Tyramine Hydrochloride Based Label‐Free System for Operating Various DNA Logic Gates and a DNA Caliper for Base Number Measurements

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