A survey of advancements in nucleic acid-based logic gates and computing for applications in biotechnology and biomedicine

Wu, Cuichen; Wan, Shuo; Hou, Weijia; Zhang, Liqin; Xu, Jin; Cui, Cheng; Wang, Yanyue; Hu, Jun; Tan, Weihong

doi:10.1039/c4cc10047f

Cited by 70 publications

(51 citation statements)

References 99 publications

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“…27,28 More importantly, aptamers can be combined with other DNA-based reactions and technologies, like Watson–Crick hybridization, polymerase chain reaction, rolling cycle reaction, and DNA-based nanotechnologies, to achieve a variety of biomedical applications on cell surfaces. 29,30 Given the similarity between cell membrane and exosome membrane surfaces, aptamer-based molecular engineering should be useful in exosome surface modifications as well, to fulfill the demands of many biomedical applications.…”

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confidence: 99%

Molecular Recognition-Based DNA Nanoassemblies on the Surfaces of Nanosized Exosomes

et al. 2017

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Exosomes are membrane-enclosed extracellular vesicles derived from cells, carrying biomolecules that include proteins and nucleic acids for intercellular communication. Owning to their advantages of size, structure, stability, and biocompatibility, exosomes have been used widely as natural nanocarriers for intracellular delivery of theranostic agents. Meanwhile, surface modifications needed to endow exosomes with additional functionalities remain challenging by their small size and the complexity of their membrane surfaces. Current methods have used genetic engineering and chemical conjugation, but these strategies require complex manipulations and have only limited applications. Herein, we present an aptamer-based DNA nanoassemblies on exosome surfaces. This in situ assembly method is based on molecular recognition between DNA aptamers and their exosome surface markers, as well as DNA hybridization chain reaction initiated by an aptamer-chimeric trigger. It further demonstrated selective assembly on target cell-derived exosomes, but not exosomes derived from nontarget cells. The present work shows that DNA nanostructures can successfully be assembled on a nanosized organelle. This approach is useful for exosome modification and functionalization, which is expected to have broad biomedical and bioanalytical applications.

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Molecular Recognition-Based DNA Nanoassemblies on the Surfaces of Nanosized Exosomes

et al. 2017

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“…Besidesn ucleic acid inputs,v ariousb ioactive moleculesh ave been chosen as targets for DNA-based biosensorsw ith a strand-displacementm echanism. [100] Stojanovic andc o-workers developed DNA-based molecular automata capable of evaluating cell surfaces. [101] In their design,c ell surfacem arkers served as inputs to direct autonomous DNA strand-displacement cascades with au nique molecular tag as the final output,i ndicating as pecific combination of marker molecules.…”

Section: Biosensingmentioning

confidence: 99%

Stimuli‐Responsive DNA Self‐Assembly: From Principles to Applications

Jin

et al. 2019

Chemistry A European J

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Stimuli‐responsive DNA self‐assembly shares the advantages of both designed stimuli‐responsiveness and the molecular programmability of DNA structures, offering great opportunities for basic and applied research in dynamic DNA nanotechnology. In this minireview, we summarize the most recent progress in this rapidly developing field. The trigger mechanisms of the responsive DNA systems are first divided into six categories, which are then explained with illustrative examples following this classification. Subsequently, proof‐of‐concept applications in terms of biosensing, in vivo pH‐mapping, drug delivery, and therapy are discussed. Finally, we provide some remarks on the challenges and opportunities of this highly promising research direction in DNA nanotechnology.

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“…A DNA computer described in 1994 achieved a 100 teraflop computing capacity (10 14 operations per second); this was more powerful than the supercomputers available at that time . The computational power of nucleic acids has evolved over the past 20 years to include broader experimental use of ‘nucleic acid‐based logic gates’ …”

Section: Nucleic Acids and Nucleic Acid Nanostructuresmentioning

confidence: 99%

Nanostructured luminescently labeled nucleic acids

2016

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Important and emerging trends at the interface of luminescence, nucleic acids and nanotechnology are: (i) the conventional luminescence labeling of nucleic acid nanostructures (e.g. DNA tetrahedron); (ii) the labeling of bulk nucleic acids (e.g. single-stranded DNA, double-stranded DNA) with nanostructured luminescent labels (e.g. copper nanoclusters); and (iii) the labeling of nucleic acid nanostructures (e.g. origami DNA) with nanostructured luminescent labels (e.g. silver nanoclusters). This review surveys recent advances in these three different approaches to the generation of nanostructured luminescently labeled nucleic acids, and includes both direct and indirect labeling methods.

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A survey of advancements in nucleic acid-based logic gates and computing for applications in biotechnology and biomedicine

Cited by 70 publications

References 99 publications

Molecular Recognition-Based DNA Nanoassemblies on the Surfaces of Nanosized Exosomes

Molecular Recognition-Based DNA Nanoassemblies on the Surfaces of Nanosized Exosomes

Stimuli‐Responsive DNA Self‐Assembly: From Principles to Applications

Nanostructured luminescently labeled nucleic acids

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