Interfacial Diffusion Printing: An Efficient Manufacturing Technique for Artificial Tubular Grafts

Abstract

Despite great progresses in bioprinting materials and technologies, immense challenges still remain when printing tubular tissues or organs with satisfying mechanical and chemical properties, such as blood vessel, colon, and trachea. Herein, a promising extrusion system based on an interfacial diffusion printing (IDP) technique for one-step printing of tubular tissue grafts is proposed. Specifically, this technique offers great convenience to prepare hollow hydrogel fibers with excellent mechanical properties and satisfactory biocompatibility. The tubular diameter can be readily adjusted within 6 mm, which renders the possibility of these hydrogel tubes to serve as small-diameter vascular grafts. In the model of animal trials, the hydrogel grafts with the capability of enduring arterial pressure are mechanically stable in rabbit carotid artery replacement. Because of its intrinsic simplicity and generality, the IDP technique is considered to be one of the reliable choices for more complicated bioengineering.

A One-pot-synthesized Double-layered Anticoagulant Hydrogel Tube

“…Thus, an inner layer of calcium alginate when the sodium alginate solutions are extruded into a calcium chloride to produce the egg-box model hydrogel may be a viable, low-cost strategy. In addition, a method of sacrificial core materials can be used to construct a hollow core, calcium alginate hydrogel [15] , as acrylamide-calcium alginate hydrogels have been shown to possess excellent mechanical properties and biocompatibility [16] .…”

Recent progress in extrusion 3D bioprinting of hydrogel biomaterials for tissue regeneration: a comprehensive review with focus on advanced fabrication techniques

“…Figure 5(c) illustrates the Schematic illustration of the inflammationmediated process for vascular remodeling and macroscopic observations of the vascular graft harvested after implantation for 1 month. Moreover, the ultrasonography clearly shows the blood flows normally at a speed of 39.4 cm/s in the grafted vascular 1 day after the implantation [236].…”

“…In a recent study, Zhou et al [236] introduced a convenient and efficient technique, designated as the interfacial diffusion for creating vascular tissue graft. In this method, hydrogel material was extruded into another medium and subjected to a diffusion gelation process.…”

Trends in Double Networks as Bioprintable and Injectable Hydrogel Scaffolds for Tissue Regeneration

“…The same authors also reported printing of a thermoresponsive alginate/poly(N-isopropylacrylamide) DN hydrogel using a similar procedure. 75 In one of the most thorough examples for applying the alginate/PAAm system in 3D printing, Zhou et al 76 printed tubes using interfacial diffusion printing (Figure 3). A bioink consisting of water, alginate, acrylamide, bacterial cellulose, MBAA, and ammonium persulfate (APS, initiator) is extruded into a reactive aqueous medium consisting of calcium chloride and tetramethylethylenediamine (TEMED, catalyst).…”