Interactive Effects of pH and Temperature on Cell Growth and Polymer Production by Xanthomonas campestris

Esgalhado, M.Eugénia

doi:10.1016/0032-9592(94)00044-i

Cited by 15 publications

(8 citation statements)

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“…These results confirm that the yield depended on incubation conditions and bacterial strain [20]. [31]. A maximum EPS yield of about 15.05 g.L -1 was achieved using Klebsiella sp.…”

Section: Eps Production Levels Optimised By (Rsm)supporting

confidence: 82%

Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization

Ermiş

Poyraz²,

Dertli³

et al. 2020

Sakarya University Journal of Science

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Response surface methodology was used to determine the exopolysaccharide production of Lactobacillus brevis E25. The effects of three variables, temperature (30, 36 and 42 °C), incubation time (18, 33 and 48 h) and initial pH of growth medium (4.5, 5.5, and 6.5) were studied. Under optimum growth conditions, the amount of exopolysaccharide derived from Lactobacillus brevis E25 ranged from 10 to 35 gL-1. The size of EPS molecules ranged from 10 5 to 10 7 Da. Infra red spectrum analysis showed characteristics absorption peaks indicating the presence of-OH, C=O and C-H groups. Furthermore, only glucose was detected as monosaccharide in exopolysaccharide structure, revealing that the structure of exopolysaccharide is a homopolymeric glucan type. Based on the differential scanning calorimeter thermograms, exopolysaccharide's melting temperature was observed around 116 °C.

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“…These results confirm that the yield depended on incubation conditions and bacterial strain [20]. [31]. A maximum EPS yield of about 15.05 g.L -1 was achieved using Klebsiella sp.…”

Section: Eps Production Levels Optimised By (Rsm)supporting

confidence: 82%

Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization

Ermiş

Poyraz²,

Dertli³

et al. 2020

Sakarya University Journal of Science

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“…Previous reports from Shu and Yang (1990) and Shu and Yang (1991) revealed higher values of EPS at 30-33°C. Similarly, Esgalhado et al (1995) referred to an optimum temperature from 25-30°C, with the highest yield of EPS (39.26 mg/L) at 27°C. At the general growth temperature of 37°C, EPS biosynthesis from Lactobacillus paracasei HCT was slightly lower (Xu et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Response Surface Optimization of Exopolysaccharide Production from Sugarcane Juice by Lactobacillus confusus TISTR 1498

Manochai¹,

Phimolsiripol²,

Seesuriyachan³

2014

CMUJNS

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Lactobacillus confusus TISTR 1498, isolated from Thai traditional fermented pork (Nham), could secrete large amounts of exopolysaccharides (EPS). Response surface methodology was applied to optimize the culture conditions for EPS production using Central Composite Design (CCD). The effects of three variables including pH (4-7), temperature (20-40°C) and nitrogen source (0.25-1.75 PYB) on EPS yield and biomass were investigated. The 1PYB was the mixtures of 5 g/L peptone (P), 2.5 g/L yeast extract (Y) and 2.5 g/L beef extract (B). Response surface methodology showed that the data were adequately fitted to a second-order polynomial model via quadratic regression relationships. The optimal culture conditions for EPS production in sugarcane juice were pH of 5.55, 29.75°C and 1.22PYB, which was composed of 6.1 g/L peptone, 3.05 g/L yeast extract and 3.05 g/L beef extract. Under the optimum condition, the predicted maximum EPS production was 107.5 g/L and the predicted biomass was 1.92 g/L. In submerged fermentation, sugarcane juice enhanced EPS yield twice as well as the modified MRS sucrose medium. In addition, the cost of medium can be lowered to 0.53 THB/g EPS, which was lower than that of the medium from the mixtures of the PYB (0.78 THB/gEPS).

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“…Out of five different fermentation media [13][14][15][16][17], media described by Kalogiannis et al [17] was found to be efficient on the basis of yield per liter of the medium. The results showed that the amount of xanthan produced on waste residual molasses was comparatively different with that of produce from glucose, sucrose, lactose, galactose and maltose (Figure 8).…”

Section: Optimization Of Xanthan Productionmentioning

confidence: 99%

Study on the Effect of pH, Temperature and Aeration on the Cellular Growth and Xanthan Production by Xanthomonas campestris Using Waste Residual Molasses

Mudoi¹,

Bharali²,

Konwar³

2013

J Bioproces Biotechniq

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Citation: Mudoi P, Bharali P, Konwar BK (2013) Study on the Effect of pH, Temperature and Aeration on the Cellular Growth and Xanthan Production by Xanthomonas campestris Using Waste Residual Molasses. J Bioprocess Biotech 3: 135 AbstractWaste residual molasses, a non-edible portion produced during the processing of sugarcane juice for the preparation of molasses, may be an alternative low-cost renewable substrate to the pricey food-grade molasses for xanthan production. Systematic strategies were applied to improve xanthan production with a newly isolated indigenous strain Xanthomonas campestris originated from Tezpur, Assam. Analyses with TLC, HPLC and FTIR show that the polymer consisted mainly of glucose, galactose and glucornic acid but showed no evidence of xylose, arabinose or glycoprotein in the polysaccharide. The isolated xanthan exhibited all the required physico-chemical characteristics and were examined by using TGA, DSC, XRD and SEM. Maximum concentration of xanthan was observed after 24h of incubation of the culture media, pH 7 at 28°C with 200 rpm. The viscosity of xanthan was found to be stable over a wide range of pH, reduced with the increase in temperature and raised at the higher xanthan concentration. The results obtained in the present investigation are noteworthy for the possible xanthan production from low-cost waste residual molasses at an industrial level.

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Interactive Effects of pH and Temperature on Cell Growth and Polymer Production by Xanthomonas campestris

Cited by 15 publications

References 0 publications

Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization

Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization

Response Surface Optimization of Exopolysaccharide Production from Sugarcane Juice by Lactobacillus confusus TISTR 1498

Study on the Effect of pH, Temperature and Aeration on the Cellular Growth and Xanthan Production by Xanthomonas campestris Using Waste Residual Molasses

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