Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization

Ermiş, Ertan; Poyraz, Ecem; Dertli, Enes; Yılmaz, Mustafa Tahsin

doi:10.16984/saufenbilder.545929

Cited by 6 publications

(4 citation statements)

References 33 publications

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“…However, in Whey and Whey-l, the EPS yields were significantly higher than in MRS-1, confirming that lactose had a positive influence on EPS production. While studies on EPS production with this strain are limited, Ermiş et al [64] observed much higher production values for L. brevis E25, which produced from 10 to 35 g/L in MRS broth.…”

Section: Discussionmentioning

confidence: 77%

Efficient Screening and Enhanced Exopolysaccharide Production by Functional Lactic Acid Bacteria (LAB) in Lactose Supplemented Media

I. E. Pintado,

C. R. Barbosa,

Pintado

et al. 2024

Applied Microbiology: Theory ＆ Technology

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Exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) can be considered as natural biological thickeners that have attracted considerable attention in the food industry. This study aimed to evaluate and select potentially EPS-producing strains LAB and to assess the influence of carbon source and aeration on EPS production. Nine LAB strains were assessed as potential EPS producers, and Rahnella aquatilis ATCC 55046 was employed as the positive control strain for EPS. The compaction test and the observation of viscous colonies in a solid medium did not yield sufficient evidence for the presence of EPS. The assessment of capsules through staining provided evidence of EPS presence only for Rahnella aquatilis ATCC 55046. The EPS yield was subsequently assessed in De-Man Rogosa and Sharpe (MRS) broth medium supplemented with 2% (w/w) fructose (MRS-f) or lactose (MRS-l), as well as in whey (Whey) and whey supplemented with 2% (w/w) lactose (Whey-l). The EPS production in the various culture media under study ranged from 194 to 1,187 mg of EPS/g of polymer dry mass (PDM). These results suggest that the culture medium and carbon sources had an impact on the EPS production of the different strains. Bifidobacterium animalisBb12 achieved the highest EPS production in MRS-f. In the case of MRS-l, the control strain recorded the highest EPS value, along with Lactobacillus acidophilus LAC-1. Regarding Whey, Lentilactobacillus Kefir NCFB 2753 exhibited the highest EPS production, while in Whey-l, Lacticaseibacillus paracasei LCS-1 emerged as the top performer in terms of EPS production. This suggests that certain strains exhibit potential for use in the production of novel fermented EPS products, whether dairy or non-dairy.

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Section: Discussionmentioning

confidence: 77%

Efficient Screening and Enhanced Exopolysaccharide Production by Functional Lactic Acid Bacteria (LAB) in Lactose Supplemented Media

I. E. Pintado,

C. R. Barbosa,

Pintado

et al. 2024

Applied Microbiology: Theory ＆ Technology

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“…The FT-IR spectrum of the EPS was determined in the range of 400–4000 cm −1 using an FT-IR spectrometer (Thermo-scientific type, model Nicolet—IS 10, USA) 47 .…”

Section: Methodsmentioning

confidence: 99%

Bio-stimulating effect of endophytic Aspergillus flavus AUMC 16068 and its respective ex-polysaccharides in lead stress tolerance of Triticum aestivum plant

EL-khawaga,

Mustafa,

El khawaga

et al. 2024

Sci Rep

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Heavy metal accumulation is one of the major agronomic challenges that has seriously threatened food safety. As a result, metal-induced phytotoxicity concerns require quick and urgent action to retain and maintain the physiological activities of microorganisms, the nitrogen pool of soils, and the continuous yields of wheat in a constantly worsening environment. The current study was conducted to evaluate the plant growth-promoting endophytic Aspergillus flavus AUMC 16,068 and its EPS for improvement of plant growth, phytoremediation capacity, and physiological consequences on wheat plants (Triticum aestivum) under lead stress. After 60 days of planting, the heading stage of wheat plants, data on growth metrics, physiological properties, minerals content, and lead content in wheat root, shoot, and grains were recorded. Results evoked that lead pollution reduced wheat plants’ physiological traits as well as growth at all lead stress concentrations; however, inoculation with lead tolerant endophytic A. flavus AUMC 16,068 and its respective EPS alleviated the detrimental impact of lead on the plants and promoted the growth and physiological characteristics of wheat in lead-contaminated conditions and also lowering oxidative stress through decreasing (CAT, POD, and MDA), in contrast to plants growing in the un-inoculated lead polluted dealings. In conclusion, endophytic A. flavus AUMC 16,068 spores and its EPS are regarded as eco-friendly, safe, and powerful inducers of wheat plants versus contamination with heavy metals, with a view of protecting plant, soil, and human health.

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“…In addition, recent evidence suggests that exopolysaccharide produced by various LAB strains is involved in potential biological activities, such as antioxidant and antibacterial properties [3,15,25,28]. Therefore, as the demand for natural polymers gradually increases in various industrial fields, research on exopolysaccharide produced by microorganisms has been conducted in recent years [1,2,9]. Many microorganisms can synthesize exopolysaccharide and excrete it out of the cell [8].…”

Section: Introductionmentioning

confidence: 99%

The Mutant Lactobacillus plantarum GNS300 Showed Improved Exopolysaccharide Production and Antioxidant Activity

Jung

Kwon

Lee

et al. 2023

Microbiol. Biotechnol. Lett.

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After random mutagenesis, the mutant Lactobacillus plantarum GNS300 showed improved exopolysaccharide production as determined by the quantification of total sugar. The mutant L. plantarum GNS300 produced 2.82 g/l of exopolysaccharide which showed 79.62% improved exopolysaccharide production compared with the parental strain. When exopolysaccharide of L. plantarum GNS300 was analyzed, the exopolysaccharide is composed of galactose (93.35%) and glucose (6.65%). Through the optimization of fermentation conditions using a bioreactor, 2.93 g/l of exopolysaccharide was produced from 20 g/l of glucose at 35℃, 500 rpm, and 0.1 vvm for 12 h. The mutant L. plantarum GNS300 exhibited 69.18% higher antioxidant activity than that from the parental strain, which might be caused by higher exopolysaccharide production. The concentrated supernatant of the mutant L. plantarum GNS300 inhibited the growth of gram-positive bacteria (Bacillus cereus and Staphylococcus aureus) and gram-negative bacteria (Escherichia coli, Vibrio parahaemolyticus, and Salmonella typhimurium).

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Optimization of exopolysaccharide production of Lactobacillus brevis E25 using RSM and characterization

Cited by 6 publications

References 33 publications

Efficient Screening and Enhanced Exopolysaccharide Production by Functional Lactic Acid Bacteria (LAB) in Lactose Supplemented Media

Efficient Screening and Enhanced Exopolysaccharide Production by Functional Lactic Acid Bacteria (LAB) in Lactose Supplemented Media

Bio-stimulating effect of endophytic Aspergillus flavus AUMC 16068 and its respective ex-polysaccharides in lead stress tolerance of Triticum aestivum plant

The Mutant Lactobacillus plantarum GNS300 Showed Improved Exopolysaccharide Production and Antioxidant Activity

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