Interactions Between Macrophages of Guinea Pigs and Salmonellae I. Fate of Salmonella typhimurium Within Macrophages of Normal Guinea Pigs

Abstract

A suspended cell culture procedure was described for the cultivation of guinea pig macrophages infected with Salmonella typhimurium. The fate of the intracellular bacteria was assessed by quantitative recovery of viable bacteria with 0.5% solution of sodium desoxycholate. Two strains of S. typhimurium with different degrees of virulence for mice were compared. There was an initial destructoin of intracellular bacteria of both strains; however, the extent of this destruction differed. Approximately 1% of the avirulent bacteria initially phagocytized survived at the end of 4 hr, whereas approximately 8% of the virulent bacteria survived at the end of 3 hr. After this initial killing, the intracellular bacteria began to multiply at a logarithmic rate between 3 and 21 hr after phagocytosis, and then a stationary phase was attained. The rate of this multiplication was comparable for both strains.

Interactions Between Salmonellae and Macrophages of Guinea Pigs IV. Relationship Between Migration Inhibition and Antibacterial Action of Macrophages

“…MATERIALS AND METHODS S. typhimurium. The stock virulent strain SR-11 (13) was used in this study. The mean lethal dose of this strain was approximately 10 bacteria upon intraperitoneal injection into mice.…”

Pathogenesis and immunity in murine salmonellosis.

“…Among the physical methods of lysing host cells, mechanical grinding (40), Ballotini beads (53), and sonic vibration (5) have been used for the release of intracellular organisms. We found that sonication could recover consistently more salmonellae than SDC or water from identical suspensions of infected macrophages (45,49).…”

“…In earlier studies, the infected cells were disintegrated by SDC, but sonic treatment was found to be more effective for the recovery of viable intracellular bacteria. Figure 1 shows the scheme used for culturing peritoneal exudative macrophages infected with S. typhimurium (49). The organisms were cultivated in tryptic soy broth (Difco Laboratories) for 6 h in a rotating drum, washed in saline, and optically adjusted to a suitable concentration of viable bacteria.…”

Comparative antibody response to salmonella antigens in genetically resistant and susceptible mice

“…It lurns out lo be a highly sensitive method to detect the presence as well as the level of antibody in mouse serum for comparative purposes. the late L. Joe Berry [5]. The organisms were grown in tryptic soy broth (Difco Laboratories.…”

“…The organisms were grown in tryptic soy broth (Difco Laboratories. Delroil, Ml) at 37 C for 6 h and washed in saline by the previously described procedure [5]. The oplically standardized bacterial stispensions contained approximately I X 10" viable organisms per ml of the strain RiA and 2 X 10'' viable organisms per ml of the strain SR-11.…”