Pathogenesis and immunity in murine salmonellosis.

Hsu, H. S.

doi:10.1128/mmbr.53.4.390-409.1989

Cited by 98 publications

(65 citation statements)

References 76 publications

(137 reference statements)

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“…The vast majority of the bacteria in the livers of mice infected with Salmonella are associated with pathological lesions (Fig. 1E and F) (Hsu, 1989;Richter-Dahlfors et al, 1997). The overall increase in bacterial numbers in the tissues is paralleled by an increased number of these lesions.…”

Section: Discussionmentioning

confidence: 91%

Dynamics of bacterial growth and distribution within the liver duringSalmonellainfection

Sheppard

Webb

Heath

et al. 2003

Cellular Microbiology

125

135

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SummarySalmonella enterica causes severe systemic diseases in humans and animals and grows intracellularly within discrete tissue foci that become pathological lesions. Because of its lifestyle Salmonella is a superb model for studying the in vivo dynamics of bacterial distribution. Using multicolour fluorescence microscopy in the mouse typhoid model we have studied the interaction between different bacterial populations in the same host as well as the dynamic evolution of foci of infection in relation to bacterial growth and localization. We showed that the growth of Salmonella in the liver results in the spread of the microorganisms to new foci of infection rather than simply in the expansion of the initial ones. These foci were associated with independently segregating bacterial populations and with low numbers of bacteria in each infected phagocyte. Using fast-growing and slow-growing bacteria we also showed that the increase in the number of infected phagocytes parallels the net rate of bacterial growth of the microorganisms in the tissues These findings suggest a novel mechanism underlying growth of salmonellae in vivo with important consequences for understanding mechanisms of resistance and immunity.

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Section: Discussionmentioning

confidence: 91%

Dynamics of bacterial growth and distribution within the liver duringSalmonellainfection

Sheppard

Webb

Heath

et al. 2003

Cellular Microbiology

125

135

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“…The differences between animal species in their susceptibility to infection are probably related to the differences in the early influx of inflammatory leucocytes into the foci of infection and their phagocytic antibacterial capacity. As these are genetically controlled in various animal species, the level of natural resistance to salmonellosis may also be genetically determined (Hormaeche et al 1983;Hsu 1986;O'Brien 1986;Vladoianu et al 1990).…”

Section: Host-salmonella Interactionsmentioning

confidence: 99%

“…typhimurium may cause extraintestinal infections (Wilkins and Roberts 1988). The physiological basis of this host specificity is still unclear (Hormaeche et al 1983;Hsu 1986;O'Brien 1986). As a result, it has proved difficult to develop an animal model to mimic the salmonella infection of humans.…”

Section: Introductionmentioning

confidence: 99%

A rat model of infection by Salmonella typhimurium or Salm. enteritidis

Naughton¹,

Grant²,

Spencer³

et al. 1996

J Appl Microbiol

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Salmonellosis in the rat has many similarities with the disease in humans, with the ileum thought to be the main site of colonization/invasion in both species. Thus, the rat may be a useful way to study the mechanism of infection by these pathogenic bacteria. A series of infection trials carried out with Hooded Lister rats showed that a salmonella infection persisted for an extended period of time and that salmonellae bind to the small intestinal epithelium as early as 4 h after intragastric intubation. Reinfection from the large intestine may not therefore initially play a significant role in the salmonella infection process. The rat model may therefore provide a means to test in vivo interventionist strategies, designed to block binding of the pathogens in the gastrointestinal tract. Road, Bucksburn, Aberdeen A B 2 9SB. UK (r-niail pjn'j"@rri.sari.ar..uk/. 0 1996 The Society for Applied Bacteriology 652 P.J. NAUGHTON E T A L . Correspondence to: Palrick J. Niiughlon, Division iflNutriiionu1 Sciences, Romeit Resrurch Institutr. Greenbiirn

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“…Systemic distribution and colonization of internal organs follows the interaction between Salmonella and the intestinal mucosa at the M-cells of the Peyer's patches (Jones et al , 1994) . Systemic infections are probably initiated by bacteria that have entered the mesenteric Iymphatics (Hohmann et al, 1978;Hsu, 1989). Large strides have been made in the identification of bacterial genes that contribute to the invasive or pathogenic phenotype of Salmonella (Mahan et al, 1993;Pace et al, 1993;Betts and Finlay, 1992;Altmeyer et al, 1993;Galan and Curtiss, 1989;Ginocchio et al, 1992;Eichelberg et al, 1994;Reitmeyer et al, 1986), and the mammalian host cell response to bacterial entry (Miller, 1991 ;Miller et al, 1992;Bliska and Falkow, 1993;Miller et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Photonic detection of bacterial pathogens in living hosts

Contag

Mullins

et al. 1995

Molecular Microbiology

534

390

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The study of pathogenic processes is often limited to ex vivo assays and cell-culture correlates. A greater understanding of infectious diseases would be facilitated by in vivo analyses. Therefore, we have developed a method for detecting bacterial pathogens in a living host and used this method to evaluate disease processes for strains of Salmonella typhimurlum that differ in their virulence for mice. Three strains of Salmonella were marked with bioluminescence through transformation with a plasmid conferring constitutive expression of bacterial luciferase. Detection of photons transmitted through tissues of animals infected with bioluminescent Salmonella allowed localization of the bacteria to specific tissues. In this manner progressive infections were distinguished from those that were persistent or abortive. We observed patterns of bioluminescence that suggested the caecum may play a pivotal role in Salmonella pathogenesis. In vivo efficacy of an antibiotic was monitored using this optical method. This study demonstrates that real time non-invasive analyses of pathogenic events and pharmacological monitoring can be performed in vivo.

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Pathogenesis and immunity in murine salmonellosis.

Cited by 98 publications

References 76 publications

Dynamics of bacterial growth and distribution within the liver duringSalmonellainfection

Dynamics of bacterial growth and distribution within the liver duringSalmonellainfection

A rat model of infection by Salmonella typhimurium or Salm. enteritidis

Photonic detection of bacterial pathogens in living hosts

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