Interaction of zearalenone and soybean isoflavone on the development of reproductive organs, reproductive hormones and estrogen receptor expression in prepubertal gilts

Wang, D.F.; Zhang, N.Y.; Peng, Yongchong; Qi, Desheng

doi:10.1016/j.anireprosci.2010.10.002

Cited by 37 publications

(25 citation statements)

References 30 publications

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“…Another study in adult male rats showed that high E 2 (10, 100 and 1,000 µg/(kg·days) via subcutaneous injection) significantly reduced serum LH and FSH in a dose‐related manner (Gillsharma et al, ). Previous study results also showed decreased serum LH levels in post‐weaning gilts challenged by ZEA 1.20 mg/kg (Fu et al, ) and 2 mg/kg (Wang, Zhang, Peng, & Qi, ). These previous findings suggested that both ZEA and EB disrupted the normal secretion of serum FSH and LH, which was consistent with our present results.…”

Section: Discussionmentioning

confidence: 60%

Comparative study of stress response, growth and development of uteri in post‐weaning gilts challenged with zearalenone and estradiol benzoate

Zhou

Yang

Chen

et al. 2019

Animal Physiology Nutrition

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The objective of this study was to evaluate the effects of zearalenone (ZEA) and estradiol benzoate (EB) on stress injury and uterine development in post‐weaning gilts. Thirty healthy post‐weaning female gilts (Duroc × Landrace × Large White) aged 28–32 days were randomly allocated to three treatments as follows: (a) basal diet (Control), (b) basal diet plus 1.0 mg/kg purified ZEA (ZEA) and (c) basal diet plus 0.75 ml (1.5 mg) EB per pig at 3‐days intervals by intramuscular injection (EB). The serum estradiol (E2), the final and the increased vulvar area, uterine index, thickness of the myometrium and endometrium, and protein expression of heat shock protein 70 (HSP70) in ZEA group were higher than those in the control group (p < .05), but lower than those in the EB group (p < .05). The serum luteinizing hormone in ZEA group was lower than that of the control group (p < .05), but higher than that in the EB group (p < .05). Higher serum follicle‐stimulating hormone and progesterone were observed in the ZEA and control groups than those in the EB group (p < .05). The serum glutathione peroxidase activity in the ZEA group was lower than that in the control and EB groups (p < .001), and the malondialdehyde in the ZEA group was higher than that in the control and EB groups (p < .001). Moreover, the relative mRNA and protein expression of growth hormone receptor (GHR) and relative mRNA expression of HSP70 in the ZEA and EB groups were higher than those in the control group (p < .05). In conclusion, both ZEA (1.0 mg/kg) and EB (1.5 mg at 3 days intervals by intramuscular injection) stimulated vulvar swelling and uterine hypertrophy by disordering serum hormones and up‐regulating GHR expression, and induced stress by different mechanisms in this study. Furthermore, the observed up‐regulating HSP70 expression challenged by ZEA or EB may be part of the mechanism to resist stress injury.

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Section: Discussionmentioning

confidence: 60%

Comparative study of stress response, growth and development of uteri in post‐weaning gilts challenged with zearalenone and estradiol benzoate

Zhou

Yang

Chen

et al. 2019

Animal Physiology Nutrition

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“…In the current study, ZEA decreased the level of luteinizing hormone (LH) in post-weaning gilts, but had no influence on the level of follicle stimulating hormone (FSH), estradiol (E2), prolactin (PRL), progesterone (PRG), or testosterone (T). Wang et al observed that ZEA decreased the levels of E2 and LH in pre-pubertal gilts, but had no effect on the level of FSH [28]. Other researchers also reported that serum LH in gilts was significantly decreased by adding ZEA in feeds [29,30].…”

Section: Discussionmentioning

confidence: 99%

Effect of Degradation of Zearalenone-Contaminated Feed by Bacillus licheniformis CK1 on Postweaning Female Piglets

Wang

et al. 2016

Toxins

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Zearalenone (ZEA), an estrogenic mycotoxin, is mainly produced by Fusarium fungi. In this study, Bacillus licheniformis CK1 isolated from soil with the capability of degrading ZEA was evaluated for its efficacy in reducing the adverse effects of ZEA in piglets. The gilts were fed one of the following three diets for 14 days: a basic diet for the control group; the basic diet supplemented with ZEA-contaminated basic diet for the treatment 1 (T1) group; and the basic diet supplemented with fermented ZEA-contaminated basic diet by CK1 for the treatment 2 (T2) group. The actual ZEA contents (analyzed) were 0, 1.20 ± 0.11, 0.47 ± 0.22 mg/kg for the control, T1, and T2 diets, respectively. The results showed that the T1 group had significantly increased the size of vulva and the relative weight of reproductive organs compared to the control group at the end of the trial. The T1 group significantly decreased the concentration of the luteinizing hormone (LH) compared with the control and T2 groups. Expression of ERβ was significantly up-regulated in the T2 group compared with the control. In addition, expression of ERβ was not different between the control and the T1 group. In summary, our results suggest that Bacillus licheniformis CK1 could detoxify ZEA in feed and reduce the adverse effects of ZEA in the gilts.

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“…Omoni and Aluko (2005) reported that UDPGT activity increased in various tissues in rats fed a high-ISO diet (0.81 mg/g) for 2 weeks. Our prior study indicated that ISO can counteract the estrogenic influence of a high dose of ZEA (2.0 mg/kg) in the diet of prepubertal gilts (Wang et al, 2010). Metabolism may accelerate the biotransformation and degradation of ZEA and its metabolites, and then decrease the estrogenic toxicity of ZEA and its metabolites.…”

Section: Discussionmentioning

confidence: 99%

Soybean isoflavone reduces the residue of zearalenone in the muscle and liver of prepubertal gilts

Wang

Zhou

Hou

et al. 2013

Animal

Self Cite

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The aim of the present study was to evaluate the protective effects of isoflavone (ISO) against zearalenone (ZEA) residues in the muscle and liver tissues of prepubertal gilts. Seventy 75-day-old, prepubertal, female pigs (Duroc 3 Landrace 3 Yorkshire, 26.5 6 0.60 kg) were allocated randomly to seven diet treatments for 21days as follows: one control group (fed the basal diet) and six groups fed the basal diet with the addition of either 0.5 or 2.0 mg/kg ZEA plus either 0, 300 or 600 mg/kg ISO. The results showed that the diet with 2.0 mg/kg ZEA added caused an increase of ZEA residue level in muscle tissue (P , 0.05), and that the addition of both 0.5 and 2.0 mg/kg ZEA increased the residue level of ZEA in the liver of prepubertal gilts (P , 0.05). Addition of 600 mg/kg ISO to 2.0 mg/kg ZEA-contaminated diet decreased the ZEA residue level in liver tissue (P , 0.05), and the addition of 300 or 600 mg/kg ISO to the 2.0 mg/kg ZEA-contaminated diet decreased the residue levels of ZEA in muscle tissue (P , 0.05). Western blot analysis demonstrated that feeding ZEA to prepubertal gilts increased their protein expression of 3a/3b-hydroxysteroid dehydrogenase (HSD; P , 0.05), and that the addition of 300 or 600 mg/kg ISO to the 2.0 mg/kg ZEA-contaminated diet decreased the protein expression of 3a/3b-HSD (P , 0.05), compared with the addition of 2.0 mg/kg ZEA alone. The results demonstrated that muscle and liver tissues retain residual ZEA when pigs are fed a diet contaminated with high concentrations of ZEA, and that the concentration of ZEA in muscle and liver tissues increased with increased amounts of ZEA in the feed. In diets contaminated with high levels of ZEA, the addition of ISO may accelerate the biotransformation and degradation of ZEA and its metabolites, and reduce the residues of ZEA in liver and muscle tissues of prepubertal gilts.Keywords: soybean isoflavone, prepubertal gilts, zearalenone residue, 3a/3b-HSD expression ImplicationsMaize and soybean are two of the principle ingredients in animal feed, and as such zearalenone (ZEA) and soybean isoflavones (ISOs) are often found together in maize-soybean meal-based feed. Our study shows that diets containing ISO may accelerate the biotransformation and degradation of ZEA and its metabolites, and thus reduce the residues of ZEA in liver and muscle tissues. These findings suggest a potential means of mitigating the negative effects of high concentration of ZEA contamination in feed.

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Interaction of zearalenone and soybean isoflavone on the development of reproductive organs, reproductive hormones and estrogen receptor expression in prepubertal gilts

Cited by 37 publications

References 30 publications

Comparative study of stress response, growth and development of uteri in post‐weaning gilts challenged with zearalenone and estradiol benzoate

Comparative study of stress response, growth and development of uteri in post‐weaning gilts challenged with zearalenone and estradiol benzoate

Effect of Degradation of Zearalenone-Contaminated Feed by Bacillus licheniformis CK1 on Postweaning Female Piglets

Soybean isoflavone reduces the residue of zearalenone in the muscle and liver of prepubertal gilts

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