Interaction of Salmonella with Phagocytes in Vitro

Morello, Josephine A.; Baker, Edgar E.

doi:10.1093/infdis/115.2.131

Cited by 21 publications

(6 citation statements)

References 19 publications

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“…The rate of intracellular multiplication, however, was comparable for both strains. Our observation of an initial intracellular destruction of salmonellae is therefore in agreement with the conclusions of several investigators (1,2,6,9).…”

Section: Resultssupporting

confidence: 93%

Interactions Between Macrophages of Guinea Pigs and Salmonellae I. Fate of Salmonella typhimurium Within Macrophages of Normal Guinea Pigs

Hsu

Radcliffe

1968

J Bacteriol

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A suspended cell culture procedure was described for the cultivation of guinea pig macrophages infected with Salmonella typhimurium. The fate of the intracellular bacteria was assessed by quantitative recovery of viable bacteria with 0.5% solution of sodium desoxycholate. Two strains of S. typhimurium with different degrees of virulence for mice were compared. There was an initial destructoin of intracellular bacteria of both strains; however, the extent of this destruction differed. Approximately 1% of the avirulent bacteria initially phagocytized survived at the end of 4 hr, whereas approximately 8% of the virulent bacteria survived at the end of 3 hr. After this initial killing, the intracellular bacteria began to multiply at a logarithmic rate between 3 and 21 hr after phagocytosis, and then a stationary phase was attained. The rate of this multiplication was comparable for both strains.

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Section: Resultssupporting

confidence: 93%

Interactions Between Macrophages of Guinea Pigs and Salmonellae I. Fate of Salmonella typhimurium Within Macrophages of Normal Guinea Pigs

Hsu

Radcliffe

1968

J Bacteriol

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“…typhirnurium only required opsonization with nonnal serum for ingestion, whereas the .Q. typhosa required opsonization by specific antil:x:xiy (65).…”

Section: It Had Been Known That Phagocytes Can Kill Bacteria Bymentioning

confidence: 98%

“…Numerous studies have been performed that deroc>nstrate the ability of salmonellae to sw:vive macrophage killing (2,12,30,50,59,64,65,89), but little attention has been directed toward defense by the FMNL.…”

Section: Explanation Of 'Ihesis Formatmentioning

confidence: 99%

Porcine neutrophil function in the presence of virulent and avirulent Salmonella choleraesuis

Roof

Kramer

1989

Veterinary Immunology and Immunopathology

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Porcine polymorphonuclear leukocytes (PMNLs) may be activated by bacteria to begin phagocytosis followed by oxidative and non-oxidative mechanisms of killing. The purpose of this study was to identify differences between virulent and avirulent Salmonella choleraesuis (S. choleraesuis) strains, 38 and 9 respectively, in their interactions with porcine PMNLs using five different assays. (1) Staphylococcus aureus (S. aureus) ingestion was determined by exposure of porcine PMNLs to a mixture of S. choleraesuis and 125I labeled S. aureus. There was a 2.98% and 22.20% decrease in S. aureus ingestion by mouse-avirulent S. choleraesuis 9 and mouse-virulent S. choleraesuis 38 respectively. (2) Iodination of proteins was done by exposing zymosan stimulated porcine PMNLs to S. choleraesuis in the presence of 125I and measuring its incorporation into porcine PMNL proteins. This assay indicated a 73.7% and 74.7% decrease in iodination by S. choleraesuis 9 and S. choleraesuis 38, respectively. (3) Cytochrome c reduction was performed by using porcine PMNLs, zymosan, and S. choleraesuis to determine the bacterial effect on superoxide anion production. S. choleraesuis 9 and S. choleraesuis 38 inhibited superoxide anion production by 78.0% and 92.6%, respectively. (4) Lactoferrin release from porcine PMNLs was measured by an ELISA using the supernatant from the cytochrome c assay. Results indicate a 52.0% and 61.0% increase in lactoferrin release by S. choleraesuis 9 and 38 respectively. (5) The bactericidal assay was performed by counting cfus of S. choleraesuis after preliminary incubation with porcine PMNLs, followed by killing of extracellular S. choleraesuis and lysis of porcine PMNLs. Survival of S. choleraesuis 9 and E. coli (control) were 7.50% and 1.37%, respectively, in contrast to 52.62% survival of the virulent S. choleraesuis 38. These results indicate that both strains inhibited protein iodination and caused a slight increase in lactoferrin release, but the virulent S. choleraesuis 38 inhibited S. aureus ingestion, cytochrome c reduction, and survived porcine PMNL killing more effectively than the avirulent S. choleraesuis 9.

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“…A more reliable method is to assay the viable intracellular bacteria. Furness (28) and Morello and Baker (79) attempted to recover viable intracellular bacteria by treating infected macrophages in individual culture tubes with distilled water. In both of these studies, the authors showed initial killing of both avirulent and virulent S. typhimuriiim within resident and stimulated peritoneal macrophages of normal mice.…”

Section: Acquired Cellular Immunitymentioning

confidence: 99%

“…We have conducted an extensive study on this issue, as described later. Water is used for this purpose by some investigators (28,79); however, it can be observed microscopically that this method can only devitalize the cells by hypotonicity but cannot disrupt them to release their intracellular bacteria as dispersed single organisms for individual colony count on bacteriological media. Chemical detergents are usually highly effective in dissolving mammalian cells, but unfortunately many bacteria are also sensitive to such a treatment.…”

Section: Introductionmentioning

confidence: 99%

Pathogenesis and immunity in murine salmonellosis.

Hsu¹

1989

Microbiological Reviews

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Interaction of Salmonella with Phagocytes in Vitro

Cited by 21 publications

References 19 publications

Interactions Between Macrophages of Guinea Pigs and Salmonellae I. Fate of Salmonella typhimurium Within Macrophages of Normal Guinea Pigs

Interactions Between Macrophages of Guinea Pigs and Salmonellae I. Fate of Salmonella typhimurium Within Macrophages of Normal Guinea Pigs

Porcine neutrophil function in the presence of virulent and avirulent Salmonella choleraesuis

Pathogenesis and immunity in murine salmonellosis.

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