Interaction of biofunctionalized gold nanoparticles with model phospholipid membranes

Abstract

The understanding of the interaction of nanoparticles with cell membranes and the penetration of these nanoobjects through cell wall is highly required for their biomedical application. In this work were aimed at the study of the interaction of gold nanoparticles with model phospholipid membranes prepared at the air/water interface in a Langmuir trough. Spherical (10 and 15 nm mean diameter) and rod-like gold (aspect ratio: 2.8) nanoparticles were synthesized and biofunctionalized with L-cysteine and L-glutathione. The gold nanoparticles were characterized by TEM images and UV-Vis absorbance measurements. The interaction of the biofunctionalized gold nanoparticles with the model monolayer membrane was studied by surface pressure versus surface area compressional isotherms and by the measurement of the change in surface pressure of a preformed model membrane. The effect of the initial surface pressure of the preformed membrane was evaluated to determine the maximum insertion pressure and synergy. We have found that the driving forces of the bioconjugated Au nanoparticle (NP) or Au nanorod (NR) penetration into the monolayer membrane is mostly determined by electrostatic interaction and orientational van der Waals forces. Monolayer films were transferred with Langmuir-Blodgett technique onto solid substrates and the nanoparticles were visualized with AFM technique.

PAMAM dendrimer - cell membrane interactions

“…Such lipid layer penetration by dendrimers has also been reported for SLB systems as discussed in the previous section. A threshold surface pressure for nanoparticle insertion has also been observed for other nanomaterials such as gold [101].…”

“…There have only been a small number of studies on interactions between PAMAM dendrimers and Langmuir-monolayer models. However, monolayers have been widely used to explore interactions with other nanoparticles, including gold [101] and silica [102] as well as pharmaceuticals [103].…”

Effect of Aminated Chitosan-Coated Fe3O4 Nanoparticles with Applicational Potential in Nanomedicine on DPPG, DSPC, and POPC Langmuir Monolayers as Cell Membrane Models

“…Such an approach allows eliminating the water evaporation effect from the Langmuir trough and even small fluctuations of the external conditions, e.g., temperature or humidity, on the time-consuming experiments. The increase of the Δπ value means the insertion of the nanoparticles into the phospholipid monolayer, while its decrease is associated with the condensation of the phospholipid molecules on the nanoparticles due to nanoparticles-phospholipid binding or their extraction from the air-water interface and the subsequent sink into the water subphase [ 49 , 57 , 67 ]. The penetration experiments were performed on the same setup and at the same experimental conditions like it took place in the case of the phospholipid compression on the subphase containing the Fe 3 O 4 -AChit nanoparticles.…”

Interactions of bioactive molecules & nanomaterials with Langmuir monolayers as cell membrane models

“…Using similar approaches and characterization techniques to those described above, the results obtained Even in the case of positively-charged NPs, electrostatic repulsion can be overshadowed by the counter ions, and be incorporated into the monolayer establishing hydrophobic interactions with the alkyl chains of the lipid and those of the capping agent and/or surfactant [270].…”