PAMAM dendrimer - cell membrane interactions

Abstract

General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. 2018 PAMAM dendrimers have been conjectured for a wide range of biomedical applications due to their tuneable physicochemical properties. However, their application has been hindered by uncertainties in their cytotoxicity, which is influenced by dendrimer generation (i.e. size and surface group density), surface chemistry, and dosage, as well as cell specificity. In this review, biomedical applications of polyamidoamine (PAMAM) dendrimers and some related cytotoxicity studies are first outlined. Alongside these in vitro experiments, lipid membranes such as supported lipid bilayers (SLBs), liposomes, and Langmuir monolayers have been used as cell membrane models to study PAMAM dendrimer-membrane interactions. Related experimental and theoretical studies are summarized, and the physical insights from these studies are discussed to shed light on the fundamental understanding of PAMAM dendrimer-cell membrane interactions. We conclude with a summary of some questions that call for further investigations.a b s t r a c t a r t i c l e i n f o Available online 27 June

Dendrimers: Amazing Platforms for Bioactive Molecule Delivery Systems

“…As previously it has mentioned, the different kinds of interactions present between dendritic systems and membranes determine the success of the nanovehicles based on dendrimers [73]. The structural and organizational of biological membranes can vary depending on composition and charged lipids.…”

“…In recent years, numerous studies about the physicochemical properties of dendrimers have been carried out since these have several outstanding physicochemical properties, which make them synthetic macromolecules with a wide range of applications [17,72,73]. Dendrimers have multifunctional surfaces that give the possibility of modifying their solubility and interactions with other molecules in different environments.…”

“…The different interactions present between dendrimers and drugs, namely electrostatic interactions, dispersion forces, hydrophobic interactions, or hydrogen bonds among others, can ensure the formation of complexes. The types of interactions are important, not only because they determine the stability of the dendrimer-drug complexes, but they are also crucial to establish the kind of mechanism of uptake of the nanoformulations into the cell's interior [73]. A cytotoxicity grade can occur depending on the type of interactions between the dendrimer and the different lipidic membranes.…”

Translating a Low‐Molecular‐Weight MRI Probe Sensitive to Amino Acid Neurotransmitters into a PAMAM Dendrimer Conjugate: The Impact of Conjugation

“…Also dendrimers with the charge‐neutral surface exhibited affirmative biocompatibility profiles and were used as carriers for a wide range of molecular imaging contrast agents . On the other hand, a positively charged surface appended with, for instance, amine groups, can be more toxic but would not interfere with the paramagnetic cations and their performance . Hence, the development of stimuli‐responsive nano‐sized conjugates requires a compromise between solubility, toxicity and sensitivity towards the target molecules.…”

Efficient Delivery of dsRNA and DNA in Cultured Silkworm Cells for Gene Function Analysis Using PAMAM Dendrimers System

“…PAMAM dendrimers, possessing well-defined structures within a nanoscale volume, have been extensively investigated as nanovectors for DNA delivery and were reported to be able to transfer RNA molecules, providing a potential platform for various drug deliveries either in DNA-or RNA-based therapeutics [13,[19][20][21]. Even though PAMAM dendrimers have been widely used in mice and humans, this application in insect cells has been limited to the best of our knowledge [22,23].…”