Interactions of bioactive molecules &amp; nanomaterials with Langmuir monolayers as cell membrane models

Nobre, Thatyane M.; Pavinatto, Felippe J.; Caseli, Luciano; Barros‐Timmons, Ana; Dynarowicz-Łątka, Patrycja; Oliveira, Osvaldo N.

doi:10.1016/j.tsf.2015.09.047

Cited by 122 publications

(67 citation statements)

References 275 publications

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“…Lipid monolayers assembled by the Langmuir technique are accepted as a model for half a membrane and have proven useful to determine mechanisms of action of many biologically-relevant materials. [22][23][24][25] By way of illustration, Langmuir monolayers were useful for introduction of a novel concept of artificial enzymes (L-zyme) based on ester hydrolysis catalyzed by pK-tuned amino groups. 26 In this work, Langmuir monolayers of the saturated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and unsaturated 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)…”

Section: Introductionmentioning

confidence: 99%

Molecular-Level Modifications Induced by Photo-Oxidation of Lipid Monolayers Interacting with Erythrosin

et al. 2016

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Incorporation into cell membranes is key for the action of photosensitizers in photomedicine treatments, with hydroperoxidation as the prominent pathway of lipid oxidation. In this paper, we use Langmuir monolayers of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) as cell membrane models to investigate adsorption of the photosensitizer erythrosin and its effect on photoinduced lipid oxidation. From surface pressure isotherms and polarization-modulated infrared reflection-absorption spectroscopy (PM-IRRAS) data, erythrosin was found to adsorb mainly via electrostatic interaction with the choline in the head groups of both DOPC and DPPC. It caused larger monolayer expansion in DOPC, with possible penetration into the hydrophobic unsaturated chains, while penetration into the DPPC saturated chains was insignificant. Easier penetration is due to the less packed DOPC monolayer, in comparison to the more compact DPPC according to the monolayer compressibility data. Most importantly, light irradiation at 530 nm made the erythrosin-containing DOPC monolayer become less unstable, with a relative surface area increase of ca. 19%, in agreement with previous findings for bioadhesive giant vesicles. The relative area increase is consistent with hydroperoxidation, supporting the erythrosin penetration into the lipid chains, which favors singlet oxygen generation close to double bonds, an important requirement for photodynamic efficiency.

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Section: Introductionmentioning

confidence: 99%

Molecular-Level Modifications Induced by Photo-Oxidation of Lipid Monolayers Interacting with Erythrosin

et al. 2016

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“…The Langmuir trough or Langmuir balance provides information about the characteristics of a monolayer on the aqueous surface by measuring the changes in surface tension upon compressing the monolayer.Lipid monolayers are formed at the air-water interface in a Langmuir trough and offer the possibility of using methodologies that are able to detect membrane drug penetration through Langmuir monolayer techniques[62] and the drug's effect on lipid monolayer structural properties by Brewster angle microscopy (BAM) [63](Figure 3). More detailed information about the application of Langmuir monolayer associated techniques to the study of interactions between biomolecules and cell membranes can be found in the following reviews[64][65][66].…”

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Biophysics in cancer: The relevance of drug-membrane interaction studies

Alves

Ribeiro

Nunes

et al. 2016

Biochimica et Biophysica Acta (BBA) - Biomembranes

164

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Lipidomics has been proving that membrane lipids play a crucial role in several cell functions and are involved in several pathologies, including cancer. In fact, beyond a scaffold where proteins and other components are embedded, the cell membrane can also act as a barrier or a target for anticancer drugs. From this point of view, the development of new chemotherapeutic agents should also take into account the role of the membrane in their activity. This Review aims to highlight the importance of anticancer drug-membrane interactions as a powerful strategy to improve cancer therapy. Biophysical techniques emerge, therefore, as essential tools to unveil such interactions.

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“…29 This technique makes possible to infer the nanomaterials organization in the subphase and changes induced in the surface structure. 30 Moreover, it allows to obtain information on the interaction of molecules with the nanomaterial at the interface. 31 Consequently, this process allows determining any possible toxic effects arising from the interaction.…”

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confidence: 99%

“…30 Moreover, it allows to obtain information on the interaction of molecules with the nanomaterial at the interface. 31 Consequently, this process allows determining any possible toxic effects arising from the interaction.…”

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confidence: 99%

“…132 However, Langmuir monolayers have some disadvantages because this model only permits the simulation of the half of the cell membrane, which is deficient to studies of molecular transport through the membrane. 30,145 The lipidic dependence in the interaction with nanomaterials is a very important question because it allows to evaluate the entry or not of these materials inside the cells. The DMPE and DPPG lipids were used to study the interaction with the AgNps-nanocapsules shown in chapter 2.…”

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Smart nanomaterials based on the photoactivated release of silver nanoparticles for bacterial control

Ballesteros¹

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Smart nanomaterials can selectively respond to a stimulus and consequently be activated in specific conditions, as a result of their interaction with electromagnetic radiation, biomolecules, pH change, etc. These nanomaterials can be produced through distinct routes and be used in artificial skin, drug delivery, and other biomedical applications. In this thesis, two smart nanosystems were developed, viz., i) nanocapsules formed by aniline (A) and chitosan (CS) (A-CS) containing silver nanoparticles (AgNPs), with an average size of 78 ± 19 nm, and ii) polycaprolactone (PCL) nanofibers, fabricated by the electrospinning technique containing AgNP into their bulk, with a diameter of 417 ± 14 nm. A novel system, based on the incorporation of the as-prepared nanocapsules onto the surface of PCL nanofibers containing AgNps (antibacterial mats), was also developed. The methodology employed avoids the direct contact of silver nanoparticles with the host and optimizes its release to the surrounding environment. The AgNPs release was triggered by exposing the nanocapsules to light at 405 nm. Consequently, the electronic energy vibration resulting from the interaction of the irradiation with the surface plasmon band (SPR) of AgNps, breaking the hydrogen bonds of the nanocapsules and releasing of AgNPs at a time of 150 s. To understand the perturbation of AgNps-Nanocapsules against bacteria, membrane models using Langmuir technique with the phospholipids 1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DPPG) and 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE) were employed, which are the main components of cell membrane of Escherichia coli (E. coli). The results suggest that DPPG has more influence on the incorporation of the nanoparticles on the cell membrane. The antibacterial properties of the nanofibers/nanomaterials mats towards E. coli and Staphylococcus aureus (S. aureus) were investigated using the Agar diffusion test for 8 samples. The experiments revealed that the samples based on nanofibers/nanocapsules and irradiation presented a radius of inhibition of 2.58 ± 0.28 mm for S. aureus and 1.78 ± 0.49 mm for E. coli. This nanosystem showed to be highly interesting for biomedical applications.

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Interactions of bioactive molecules & nanomaterials with Langmuir monolayers as cell membrane models

Cited by 122 publications

References 275 publications

Molecular-Level Modifications Induced by Photo-Oxidation of Lipid Monolayers Interacting with Erythrosin

Molecular-Level Modifications Induced by Photo-Oxidation of Lipid Monolayers Interacting with Erythrosin

Biophysics in cancer: The relevance of drug-membrane interaction studies

Smart nanomaterials based on the photoactivated release of silver nanoparticles for bacterial control

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