Interaction Analysis of the Carcinoembryonic Antigen (CEA) with Its Monoclonal Antibody Immobilized on a Gold Surface Using Fourier Transform Infrared Reflection-Absorption Spectroscopy (FT-IR RAS)

Ohtsuka, Keiichi; Kuroki, Masahide; Nojima, Takahiko; Waki, Michinori; Takenaka, Shigeori

doi:10.2116/analsci.21.215

Cited by 5 publications

(4 citation statements)

References 16 publications

(19 reference statements)

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“…amide I at 1604 cm À1 . The bands at 1700-1750 cm À1 are due to the -COOH groups of the antibody 40,41 thus conrming antibody immobilization by covalent attachment and formation of the amide bond 42 (Fig. 6).…”

Section: Analysis By Atr-ftirmentioning

confidence: 99%

Studies on varying n-alkanethiol chain lengths on a gold coated surface and their effect on antibody–antigen binding efficiency

et al. 2015

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Self-assembled monolayer (SAM) of n-alkanethiols of different chain lengths (n ¼ 2, 3, 6, 11, 16) on a gold surface are used to immobilize antibodies which in turn bind to a antigen. The antibody and antigen used in this study have similar molecular weights i.e. $150 kDa. The antibody [1.5 mg cm À2 ] immobilized varied with the surface packing density of the SAM of carboxylic acid-terminated n-alkanethiols of different lengths. In comparison, the efficiency of antibody immobilization was lowest on the loosely packed SAM of n-alkanethiols (n # 3) and the highest on the densely packed SAM of n-alkanethiols (n $ 11). However, increased immobilization of antibodies with increasing chain length of the n-alkanethiols [n > 11], did not result in a corresponding increase in antigen binding. An attempt to explain this phenomenon based on packing density and an orientation of the captured antibody is presented.www.rsc.org/advances 80480 | RSC Adv., 2015, 5, 80480-80487This journal is

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Section: Analysis By Atr-ftirmentioning

confidence: 99%

Studies on varying n-alkanethiol chain lengths on a gold coated surface and their effect on antibody–antigen binding efficiency

et al. 2015

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“…The peaks at 1,744 and 1,789 cm -1 correspond to the asymmetric and symmetric stretching of the NHS carbonyl groups, respectively. The peak at 1,824 cm -1 , which represents the carbonyl stretching of the ester formed by the NHS/MUA bond, demonstrates that the NHS is indeed covalently bonded to the MUA (Ohtsuka et al 2005). …”

Section: Modification Of a Cooh-terminated Mua Layer With N-hydroxysumentioning

confidence: 97%

An electrochemical method for functionalization of a 316L stainless steel surface being used as a stent in coronary surgery: irreversible immobilization of fibronectin for the enhancement of endothelial cell attachment

et al. 2012

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An electrochemistry-based method for the formation of functionalized alkanethiol layers on a 316L stainless steel surface was developed. The method was efficient in forming a very stable, irreversibly-attached COOH-terminated (mercaptoundecanoic acid) surface layer. This layer was used as a 'linker' to immobilize the extracellular matrix protein fibronectin to the 316L stainless steel surface. Fibronectin was irreversibly attached to the surface and, unlike physisorbed fibronectin, resisted detachment more in aggressive 0.1 M NaOH under sonication. The fibronectin-modified 316L stainless steel surface was more biocompatible towards attachment of endothelial cells than a bare (unmodified) 316L stainless steel surface, yielding a 25% improvement in cell density.

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“…The PEG6000 system was the most sensitive, and was chosen for use. Compared to the assays reported, [8][9][10][11][12][13][14][15][16][17][18][19][20][25][26][27] this assay is simple and rapid, sensitive and selective.…”

Section: Working Curvementioning

confidence: 99%

“…25 A quartz crystal microbalance immunogold sensor was used to detect CEA, with a linear range of 3.0-50 ng/mL and DL of 1.5 ng/mL. 26 A Fourier transform infrared reflection-absorption spectroscopy (FT-IR RAS) based on the nanogold-labeled immunoreaction was reported to assay CEA with high sensitivity, 27 but the cost is high. Thus, it is necessary to develop a sensitive, selective, simple bioassay for CEA, with safe and stable reagent, and low-cost.…”

Section: Introductionmentioning

confidence: 99%

A Rapid and Highly Selective Resonance Scattering Spectral Assay for Trace Carcinoembryonic Antigen

Jiang

Deng

2008

Chin. J. Chem.

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Gold nanoparticles, in size of 10 nm, were used to label monoclonal carcinoembryonic antigen antibody (CEAAb) to obtain an immunonanogold resonance scattering (RS) spectral probe (AuCEAAb) for the measurement of trace carcinoembryonic antigen (CEA). In pH 6.8 Na 2 HPO 4 -NaH 2 PO 4 buffer solution and the presence of polyethylene glycol (PEG)-6000, the AuCEAAb combined with CEA to form the nanogold-labeled immunocomplex clusters in average size of 227 nm, which exhibited two scattering peaks at about 320 and 581 nm. The enhanced RS intensity at 581 nm (∆I 581 nm ) is proportional to CEA concentration in the range of 1.0-50.0 ng/mL, with a detection limit (DL) of 0.52 ng/mL. The immunonanogold RS assay was utilized to determine CEA in serum samples with high sensitivity, good selectivity and simplicity.

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Interaction Analysis of the Carcinoembryonic Antigen (CEA) with Its Monoclonal Antibody Immobilized on a Gold Surface Using Fourier Transform Infrared Reflection-Absorption Spectroscopy (FT-IR RAS)

Cited by 5 publications

References 16 publications

Studies on varying n-alkanethiol chain lengths on a gold coated surface and their effect on antibody–antigen binding efficiency

Studies on varying n-alkanethiol chain lengths on a gold coated surface and their effect on antibody–antigen binding efficiency

An electrochemical method for functionalization of a 316L stainless steel surface being used as a stent in coronary surgery: irreversible immobilization of fibronectin for the enhancement of endothelial cell attachment

A Rapid and Highly Selective Resonance Scattering Spectral Assay for Trace Carcinoembryonic Antigen

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