Inter-Study Precision of Cancer Cell Radiosensitivity As Assessed By Colony Formation Assay

Matsui, Toshimitsu; Oike, Tsubasa; Nuryadi, Endang; Nakano, Toshiko

doi:10.1016/j.ijrobp.2019.06.978

Cited by 3 publications

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“…Media were replaced every 3 days. When colonies were formed, we removed media and added 1 ml 4% paraformaldehyde to each well to fix cells for 30 min and stained them with crystal violet solution ( 22 ). Finally, colonies were counted.…”

Section: Methodsmentioning

confidence: 99%

SPAG5 Is Involved in Human Gliomagenesis Through the Regulation of Cell Proliferation and Apoptosis

Wang

Cheng

et al. 2021

Front. Oncol.

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BackgroundGlioma is the most frequent malignant primary brain tumor in adults.ObjectiveTo explore the role of sperm-associated antigen 5 (SPAG5) in glioma.MethodsThe association between SPAG5 expression and clinical features was investigated based on The Cancer Genome Atlas (TCGA) datasets. The function of SPAG5 in glioma was analyzed using U87 and U251 cells. Knockdown glioma cells were constructed by shRNA interference. qRT-PCR and Western blotting were used to measure the expression of SPAG5 and Cadherin 2 (CDH2). Cell proliferation and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, caspase 3/7 assay, and high-content screening (HCS) proliferation analysis and colony formation assay. Transwell assays and wound-healing assays were used to investigate cell migration and invasion.ResultsThe increased expression of SPAG5 was correlated with poor outcomes in glioma patients. Knocking down SPAG5 could inhibit the proliferation and colony formation and promoted the apoptosis of glioma cells. Knocking down SPAG5 could also inhibit cell migration and invasion and the expression of CDH2. Overexpression of CDH2 with SPAG5 depletion could restore the proliferation and inhibit the apoptosis of glioma cells, which also promoted cell migration and invasion.ConclusionsSPAG5 is a promising prognostic factor and potential therapeutic target for clinical intervention in glioma.

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Section: Methodsmentioning

confidence: 99%

SPAG5 Is Involved in Human Gliomagenesis Through the Regulation of Cell Proliferation and Apoptosis

Wang

Cheng

et al. 2021

Front. Oncol.

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Design, synthesis, and biological evaluation of a series of benzofuran[3,2-d]pyrimidine-4(3H)-one derivatives containing thiosemicarbazone analogs as novel PARP-1 inhibitors

Wang,

Li,

et al. 2023

Bioorganic Chemistry

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Design, Synthesis, and Biological Evaluation of a Series of Benzofuran [3,2-D]Pyrimidine-4(1h)-Ketone Derivatives Containing Thiosemicarbazone Analogs as Novel Parp-1 Inhibitors

Wang,

Li,

et al. 2023

Preprint

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Inter-Study Precision of Cancer Cell Radiosensitivity As Assessed By Colony Formation Assay

Abstract: following irradiation to the ovaries. Validation of these findings is being tested in a clinical trial of PRT for young women with tumors close to the ovaries.

Cited by 3 publications

References 0 publications

SPAG5 Is Involved in Human Gliomagenesis Through the Regulation of Cell Proliferation and Apoptosis

SPAG5 Is Involved in Human Gliomagenesis Through the Regulation of Cell Proliferation and Apoptosis

Design, synthesis, and biological evaluation of a series of benzofuran[3,2-d]pyrimidine-4(3H)-one derivatives containing thiosemicarbazone analogs as novel PARP-1 inhibitors

Design, Synthesis, and Biological Evaluation of a Series of Benzofuran [3,2-D]Pyrimidine-4(1h)-Ketone Derivatives Containing Thiosemicarbazone Analogs as Novel Parp-1 Inhibitors

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