SPAG5 Is Involved in Human Gliomagenesis Through the Regulation of Cell Proliferation and Apoptosis

Wang, Chunhong; Su, Housheng; Cheng, Rui; Ji, Hongming

doi:10.3389/fonc.2021.673780

Cited by 6 publications

(5 citation statements)

References 32 publications

(38 reference statements)

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“…[ 15 , 16 ] Dysfunction or dysregulation of SPAG5 is associated with cancer, with high levels of SPAG5 expression linked to increased proliferation, migration, and invasion of tumor cells, potentially leading to rapid tumor growth and spread. [ 17 ] In terms of tumor immunity, high expression of SPAG5 correlates with an immunosuppressive tumor microenvironment and immune therapy efficacy indicators. SPAG5 serves as a diagnostic, prognostic, and immunological marker in various cancers and may offer new targets for tumor immunotherapy.…”

Section: Discussionmentioning

confidence: 99%

SPAG5 and ASPM play important roles in gastric cancer: An observational study

Xue,

Ma,

Shan

et al. 2024

Medicine

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Gastric cancer typically originates from the abnormal proliferation of normal cells within the gastric mucosa, eventually forming tumors. The roles of sperm-associated antigen 5 (SPAG5) and abnormal spindle-like microcephaly (ASPM) associated genes in gastric cancer are not yet clear. Gastric cancer datasets GSE51575 and GSE36076 profiles were downloaded from the GPL13607 and GPL570-generated gene expression omnibus database. The analysis included filtering for differentially expressed genes, weighted gene co-expression network analysis, functional enrichment analysis, gene set enrichment analysis, immune infiltration analysis, construction and analysis of the protein–protein interaction network, survival analysis, and Comparative Toxicogenomics Database analysis. Heatmaps of gene expression were also created. A total of 1457 differentially expressed genes were identified. According to gene ontology analysis, they are primarily enriched in the metabolic processes of organic acids, condensed chromosome centromere regions, and oxidoreductase activity. Kyoto Encyclopedia of Gene and Genome analysis showed they are mainly involved in metabolic pathways, P53 signaling pathway, and PPAR signaling pathway. The soft threshold power for weighted gene co-expression network analysis was set to 8. Three core genes (CENPE, SPAG5, and ASPM) were identified. Heatmaps of core gene expression revealed that SPAG5 and ASPM are highly expressed in gastric cancer samples and low in normal samples. Comparative Toxicogenomics Database analysis indicated that the core genes (CENPE, SPAG5, and ASPM) are associated with gastric tumors, gastric diseases, gastritis, gastric ulcers, tumors, inflammation, and necrosis. The SPAG5 and ASPM genes are overexpressed in gastric cancer tissues, and higher expression levels are associated with worse prognosis, may serve as potential prognostic markers.

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Section: Discussionmentioning

confidence: 99%

SPAG5 and ASPM play important roles in gastric cancer: An observational study

Xue,

Ma,

Shan

et al. 2024

Medicine

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“…SPAG5 also promotes the invasion of breast cancer cells by activating Wnt/β-catenin signaling 18 . SPAG5 participates in human gliomagenesis by regulating cell proliferation and apoptosis 19 . SPAG5 upregulation predicts poor prognosis in patients with cervical cancer and alters sensitivity to taxol therapy via the mTOR signaling pathway 20 .…”

Section: Introductionmentioning

confidence: 99%

Upregulation of sperm-associated antigen 5 expression in endometrial carcinoma was associated with poor prognosis and immune dysregulation, and promoted cell migration and invasion

Chen,

Wang,

et al. 2024

Sci Rep

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Sperm-associated antigen 5 (SPAG5) regulates cancer cell invasion and is involved in the progression of many cancers. However, the role of SPAG5 in endometrial carcinoma (EC) is still unknown. The purpose of this study was to explore the role of SPAG5 in EC and its potential molecular mechanism. The UALCAN tool and cBioPortal were used to analyze the expression and alterations of SPAG5 in EC, respectively. OncoLnc was used for survival analysis. We analyzed the effects of SPAG5 on immune cell infiltration and the expression levels of immune checkpoints. We also overexpressed and knocked down SPAG5 in EC cells to explore the effect of SPAG5 regulation on migration, invasion, apoptosis, and the cell cycle of EC cells. We found that SPAG5 was overexpressed and the SPAG5 gene was often mutated in EC. High SPAG5 expression was significantly associated with poor overall survival in patients with EC. SPAG5 also affected the level of immune cell infiltration in the TIME and the expression of immune checkpoints lymphocyte activating 3 (LAG3) and T cell immunoreceptor with Ig and ITIM domains (TIGIT) in patients with EC. It may also be involved in the immunotherapy response in these patients. In vitro experiments showed that SPAG5 promotes cancer cell migration and invasion. In conclusion, this study lays the foundation for further understanding the molecular mechanisms of EC involving SPAG5 and contributes to diagnosing and managing this disease.

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“…SPAG5 localizes to centrosomes to participate in mitotic spindle formation and sister chromatid separation, promote centriole replication, and maintain centrosome integrity during interphase mitosis 6,7 . There is substantial evidence indicating that SPAG5 exhibits high expression levels in various tumors, including breast cancer, 8 cervical cancer, 9 ovarian cancer, 10 hepatocellular carcinoma, 11 uroepithelial bladder carcinoma, 12 and glioma 13 . Moreover, elevated SPAG5 expression is associated with cancer cell proliferation, metastasis, chemotherapy resistance, and poorer overall survival.…”

Section: Introductionmentioning

confidence: 99%

“…6,7 There is substantial evidence indicating that SPAG5 exhibits high expression levels in various tumors, including breast cancer, 8 cervical cancer, 9 ovarian cancer, 10 hepatocellular carcinoma, 11 uroepithelial bladder carcinoma, 12 and glioma. 13 Moreover, elevated SPAG5 expression is associated with cancer cell proliferation, metastasis, chemotherapy resistance, and poorer overall survival. Conversely, the downregulation of SPAG5 and its associated signaling pathways inhibits cancer cell growth, promotes apoptosis, and enhances the therapeutic sensitivity of chemotherapeutic agents.…”

Section: Introductionmentioning

confidence: 99%

Pan‐cancer analysis: SPAG5 is an immunological and prognostic biomarker for multiple cancers

Gao,

Bu,

Gao

et al. 2023

The FASEB Journal

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Sperm‐associated antigen 5 (SPAG5) is a mitotic spindle protein that regulates the separation of sister chromatids into daughter cells. Recent studies have discovered its overexpression in various cancers, suggesting its oncogenic characteristics and functions. However, a comprehensive analysis of SPAG5 regarding its diagnostic, prognostic, and immune‐related effects across different cancer types is lacking. In this study, we employed bioinformatics methods and integrated multiple public databases to explore the potential oncogenic role of SPAG5. We analyzed its expression, prognosis, related chemicals, enriched pathways, immune infiltration, and its impact on different tumor genetic alterations. The results revealed that SPAG5 is highly expressed in most cancers and significantly correlates with poor patient prognosis. Additionally, SPAG5 expression showed potential for early cancer diagnosis in 15 different cancer types. In terms of tumor immunity, high expression of SPAG5 was associated with an immunosuppressive tumor microenvironment and immune therapy efficacy indicators. SPAG5 expression exhibited a negative correlation with most immune cell infiltrates but demonstrated a significant positive correlation with Th2 cells and MDSC cells. Multicolor fluorescence immunohistochemistry demonstrated that SPAG5 activates immune cell populations within tumors, indicating its significant role in the tumor microenvironment. Enrichment analysis indicated that SPAG5‐related genes are mainly involved in cell cycle, cellular senescence, P53 signaling pathway, and FoxO signaling pathway. Furthermore, we confirmed the high expression of SPAG5 in cancer cells and observed that its knockdown upregulated the expression of the p53 protein. In conclusion, SPAG5 holds value as a diagnostic, prognostic, and immune biomarker in various cancers and may provide a novel target for tumor immunotherapy.

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SPAG5 Is Involved in Human Gliomagenesis Through the Regulation of Cell Proliferation and Apoptosis

Cited by 6 publications

References 32 publications

SPAG5 and ASPM play important roles in gastric cancer: An observational study

SPAG5 and ASPM play important roles in gastric cancer: An observational study

Upregulation of sperm-associated antigen 5 expression in endometrial carcinoma was associated with poor prognosis and immune dysregulation, and promoted cell migration and invasion

Pan‐cancer analysis: SPAG5 is an immunological and prognostic biomarker for multiple cancers

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