Integrin β1 in Adipose-Derived Stem Cells Accelerates Wound Healing via Activating PI3K/AKT Pathway

Wang, Qihong; Zhang, Na; Hu, Lihua; Yang, Xi; Mi, Wenxin; Ma, Yindong

doi:10.1007/s13770-019-00229-4

Cited by 15 publications

(11 citation statements)

References 29 publications

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“…ASC-exos might be safer, cheaper, and more effective OA therapy ( Pers et al, 2015 ). ASC-exos are able to downregulate inflammation and oxidative stress, which might successfully mediate antisenescence in OA ( Wang Q. et al, 2020 ). The intra-articular injection of ASC-exos could inhibit cartilage and subchondral bone degradation, decrease osteophyte formation, and anti-synovial inflammation, thus slowing the progression of OA ( Zhang R. et al, 2019 ).…”

Section: Asc-exos In Bone Tissue Damagementioning

confidence: 99%

Exosomes From Adipose-Derived Stem Cells: The Emerging Roles and Applications in Tissue Regeneration of Plastic and Cosmetic Surgery

Xiong

Zhang

et al. 2020

Front. Cell Dev. Biol.

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Adipose-derived stem cells (ASCs) are an important stem cell type separated from adipose tissue, with the properties of multilineage differentiation, easy availability, high proliferation potential, and self-renewal. Exosomes are novel frontiers of intercellular communication regulating the biological behaviors of cells, such as angiogenesis, immune modulation, proliferation, and migration. ASC-derived exosomes (ASC-exos) are important components released by ASCs paracrine, possessing multiple biological activities. Tissue regeneration requires coordinated "vital networks" of multiple growth factors, proteases, progenitors, and immune cells producing inflammatory cytokines. Recently, as cell-to-cell messengers, ASC-exos have received much attention for the fact that they are important paracrine mediators contributing to their suitability for tissue regeneration. ASC-exos, with distinct properties by encapsulating various types of bioactive cargoes, are endowed with great application potential in tissue regeneration, mechanically via the migration and proliferation of repair cells, facilitation of the neovascularization, and other specific functions in different tissues. Here, this article elucidated the research progress of ASC-exos about tissue regeneration in plastic and cosmetic surgery, including skin anti-aging therapy, dermatitis improvement, wound healing, scar removal, flap transplantation, bone tissue repair and regeneration, obesity prevention, fat grafting, breast cancer, and breast reconstruction. Deciphering the biological properties of ASC-exos will provide further insights for exploring novel therapeutic strategies of tissue regeneration in plastic and cosmetic surgery.

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Section: Asc-exos In Bone Tissue Damagementioning

confidence: 99%

Exosomes From Adipose-Derived Stem Cells: The Emerging Roles and Applications in Tissue Regeneration of Plastic and Cosmetic Surgery

Xiong

Zhang

et al. 2020

Front. Cell Dev. Biol.

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“…The PI3K/Akt signaling pathway is instrumental in cancer progression and also indispensable for signal transduction in normal cells [ 20 ], which also generates critical effects in many cell functions, such as metabolism, proliferation, migration, invasion, and angiogenesis [ 21 ]. Recent studies have demonstrated that PI3K/Akt signaling pathway participates in wound healing in viv o [ 22–26 ] and exerts a regulatory role in ADSCs during wound healing [ 27 ]. Zhang et al .…”

Section: Introductionmentioning

confidence: 99%

VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway

Jiang

Zhang

et al. 2021

Bioengineered

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Chronic skin ulcers are a primary global health problem. Velvet antler polypeptide (VAP) regulates endothelial cell migration and angiogenic sprout. Adipose-derived stem cells (ADSCs) are reported to make pivotal impacts upon wound healing. This study aimed to explore the role of VAP combined with ADSCs in wound healing of chronic skin ulcers. The effect of VAP on phenotypes of ADSCs, and VAP (PLGA microspheres) combining with ADSCs on wound healing of chronic skin ulcers in vivo was evaluated. VAP generally promoted the proliferation, migration and invasion of ADSCs, and ADSC-induced angiogenesis in human umbilical vein endothelial cells (HUVECs) through PI3K/Akt/HIF-1α pathway. VAP-PLGA (PLGA microspheres) enhanced the promoting effect of ADSCs on wound healing, pathological changes, and angiogenesis in chronic skin ulcers in vivo . VAP-PLGA intensified the effect of ADSCs on up-regulating the levels of p-PI3K/PI3K, p-Akt/Akt, HIF-1α, vascular endothelial growth factor (VEGF), stromal cell-derived factor-1 (SDF-1), C-X-C motif chemokine receptor 4 (CXCR4), angiopoietin-4 (Ang-4), VEGF receptor (VEGFR), and transforming growth factor-β1 (TGF-β1), and down-regulating the levels of interleukin-1 β (IL-1β), IL-18 and IL-6 in wound tissues in chronic skin ulcers in vivo . Collectively, VAP promoted the growth, migration, invasion, and angiogenesis of ADSCs through activating PI3K/Akt/HIF-1α pathway, and VAP-PLGA enhanced the function of ADSCs in promoting wound healing in vivo , which was associated with angiogenesis, inflammation inhibition, and dermal collagen synthesis.

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“…According to previous studies, transforming growth factor beta (TGF-β), insulin-like growth factor I, and interleukins are overexpressed in keloid tissues 4 – 9 . Vascular endothelial growth factor (VEGF) expression is also elevated in keloid tissues and appears to promote keloid growth 3 , 8 .…”

Section: Introductionmentioning

confidence: 99%

“…c-mesenchymal-epithelial transition factor (c-Met) is a transmembrane tyrosine kinase that acts as a receptor for hepatocyte growth factor (HGF). The HGF/c-Met pathway activates the downstream extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3-kinase (PI3K) pathway, eventually driving cell proliferation and motility 8 , 10 . c-Met and HGF are dysregulated in cancer cells, and they are believed to contribute to tumor invasion, making the pathway an attractive candidate for targeted cancer therapy 7 , 9 , 11 .…”

Section: Introductionmentioning

confidence: 99%

A selective small-molecule inhibitor of c-Met suppresses keloid fibroblast growth in vitro and in a mouse model

Choi

Kim

et al. 2021

Sci Rep

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Keloids, tumor-like lesions that result from excessive scar formation, have no definitive treatment modality. Activation of c-mesenchymal-epithelial transition factor (c-Met) promotes cell proliferation and survival. Selective c-Met inhibitors, such as PHA-665752, may attenuate the activity of keloid fibroblasts and reduce keloid formation. Here, we aimed to evaluate the effect of PHA-665752, a second-generation selective small-molecule inhibitor of c-Met, on human keloid fibroblasts in vitro and in a mouse model. We performed in vitro cytotoxicity assays, scratch tests, western blotting, and immunofluorescence on human keloid fibroblasts. We also injected human fibroblasts into severe combined immunodeficient mice and measured the degree of nodule formation and skin histologic characteristics. We found that keloid fibroblast migration was inhibited by PHA-665752. Inhibitor treatment was also associated with lower expression of members of the hepatocyte growth factor/c-Met pathway, and lower fibroblast activity and collagen synthesis. In the in vivo experiments, PHA-665752—treated mice had lower nodule volumes and weights, accompanied by less inflammatory cell infiltration and collagen deposition, than those in control mice. These findings showed that although an in vivo model may not accurately represent the pathophysiology of human keloid development, PHA-665752 suppressed keloid fibroblast activity by inhibiting the c-Met—related tyrosine kinase pathway.

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Integrin β1 in Adipose-Derived Stem Cells Accelerates Wound Healing via Activating PI3K/AKT Pathway

Cited by 15 publications

References 29 publications

Exosomes From Adipose-Derived Stem Cells: The Emerging Roles and Applications in Tissue Regeneration of Plastic and Cosmetic Surgery

Exosomes From Adipose-Derived Stem Cells: The Emerging Roles and Applications in Tissue Regeneration of Plastic and Cosmetic Surgery

VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway

A selective small-molecule inhibitor of c-Met suppresses keloid fibroblast growth in vitro and in a mouse model

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