Integrative Repair of Cartilage with Articular and Nonarticular Chondrocytes

Johnson, Timothy S.; Xu, Jiake; Zaporojan, Victor; Mesa, John; Weinand, Christian; Randolph, Mark A.; Bonassar, Lawrence J.; Winograd, Jonathan M.; Yaremchuk, Michael J.

doi:10.1089/ten.2004.10.1308

Cited by 68 publications

(32 citation statements)

References 27 publications

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“…Furthermore, articular chondrocytes have been found to produce sufficient extracellular matrix including collagen type I and type II and we have shown that these cells can promote a healing response between cartilage discs. [25][26][27] Additionally, cells can be obtained from the patient's joint using minimally invasive techniques. Clinically, autologous articular chondrocyte implantation has already been widely used for the treatment of articular cartilage defects, and a similar methodology could be easily adopted for cell-guided meniscal repair therapy.…”

Section: Discussionmentioning

confidence: 99%

Implant‐assisted meniscal repair in vivo using a chondrocyte‐seeded flexible PLGA scaffold

Yoo

Bichara

Zhao

et al. 2011

J Biomedical Materials Res

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A cell-based engineered construct can be used for healing of intractable meniscal lesions. Our aims were to assess the culture conditions (static versus dynamic oscillation) and the healing capacity of the chondrocyte-seeded flexible implants in a heterotopic mouse model. Swine articular chondrocytes were labeled with PKH 26 or DiI dye and seeded onto a flexible PLGA scaffold using dynamic oscillating conditions for 24 h. Half of cell-seeded scaffolds were cultured in the same dynamic conditions, while the remaining scaffolds were cultured statically. After 7 days, scaffolds were placed between swine meniscal discs and were implanted subcutaneously in nude mice for 6 weeks. Additional constructs for assessing in vivo cell tracking were implanted for 12 weeks. Live/dead assays demonstrated labeled chondrocytes attached throughout the scaffold in both culture conditions. DNA measurements showed no significant difference between the culture conditions. A continuous fibro-cartilaginous healing tissue was observed between meniscal discs in all 12 dynamically cultured constructs and 9 of 11 statically cultured ones. There was no evidence of meniscal healing using acellular scaffold as well as in meniscal constructs lacking an implant. Both PKH 26- and DiI-labeled cells were identified along the healing interface. We conclude the chondrocyte-seeded flexible PLGA implants induce healing of meniscal discs in nude mice. Culture conditions after seeding have no apparent effects on healing.

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Section: Discussionmentioning

confidence: 99%

Implant‐assisted meniscal repair in vivo using a chondrocyte‐seeded flexible PLGA scaffold

Yoo

Bichara

Zhao

et al. 2011

J Biomedical Materials Res

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“…This finding was attributed to the proliferative capacity, mitotic division and ECM synthesis induced by the accelerated metabolism of chondrocytes [5,13,14]. However, the absence of blood supply limits the capacity of cartilage repair in older animals after different types of injuries [1,12,14,15]. Articular surface defects that do not penetrate the subchondral bone do not heal spontaneously.…”

Section: Introductionmentioning

confidence: 99%

“…Electrical and electromagnetic stimulation and autologous grafts of chondrocytes, mesenchymal cells and biocompatible tissue derived from the periosteum and perichondrium show a marked chondrogenic potential [12,24]. However, in contrast to electrical stimulation, the implantation of cells and tissues of different origins requires invasive procedures [14,15]. Within this context, electrical stimulation as a therapeutic strategy for cartilage repair has been little investigated [6,25,26] and knowledge about the response of cartilage tissue of different anatomical origins to treatment with physical agents, particularly the application of electrical currents of different intensities and for different periods of time, is scarce [25,27].…”

Section: Introductionmentioning

confidence: 99%

Effects of microcurrent stimulation on Hyaline cartilage repair in immature male rats (Rattus norvegicus)

Ciccone

Zuzzi²,

Neves³

et al. 2013

BMC Complement Altern Med

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BackgroundIn this study, we investigate the effects of microcurrent stimulation on the repair process of xiphoid cartilage in 45-days-old rats.MethodsTwenty male rats were divided into a control group and a treated group. A 3-mm defect was then created with a punch in anesthetized animals. In the treated group, animals were submitted to daily applications of a biphasic square pulse microgalvanic continuous electrical current during 5 min. In each application, it was used a frequency of 0.3 Hz and intensity of 20 μA. The animals were sacrificed at 7, 21 and 35 days after injury for structural analysis.ResultsBasophilia increased gradually in control animals during the experimental period. In treated animals, newly formed cartilage was observed on days 21 and 35. No statistically significant differences in birefringent collagen fibers were seen between groups at any of the time points. Treated animals presented a statistically larger number of chondroblasts. Calcification points were observed in treated animals on day 35. Ultrastructural analysis revealed differences in cell and matrix characteristics between the two groups. Chondrocyte-like cells were seen in control animals only after 35 days, whereas they were present in treated animals as early as by day 21. The number of cuprolinic blue-stained proteoglycans was statistically higher in treated animals on days 21 and 35.ConclusionWe conclude that microcurrent stimulation accelerates the cartilage repair in non-articular site from prepuberal animals.

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“…Additionally, fibrin has shown to be an excellent scaffold for cell delivery and tissue ingrowth in a number of tissue engineering applications (for review, see Ahmed et al ., 2008). Of specific relevance to connective tissues such as the AF, fibrin has been used to engineer muscle (Hecker et al , 2005; Huang et al , 2005; Nieponice et al , 2007; Rowe et al , 2007), skin (Hojo et al , 2003; Balestrini and Billiar, 2006;), cartilage (Passaretti et al , 2001; Connelly et al , 2004; Johnson et al , 2004; Mesa et al , 2006; Peretti et al , 2006; Eyrich et al , 2007), and connective tissue (Chong et al , 2007; Hankemeier et al , 2007). Recently, fibrin has been shown to be an effective carrier to deliver stem cells to denucelated discs (Allon et al , 2010) and to improve the structure and function of surgically damaged IVDs (Buser et al , 2011).…”

Section: Introductionmentioning

confidence: 99%

Genipin-crosslinked fibrin hydrogels as a potential adhesive to augment intervertebral disc annulus repair

Schek

Michalek²,

Iatridis³

2011

eCM

118

104

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Treatment of damaged intervertebral discs is a signifi cant clinical problem and, despite advances in the repair and replacement of the nucleus pulposus, there are few effective strategies to restore defects in the annulus fi brosus. An annular repair material should meet three specifi cations: have a modulus similar to the native annulus tissue, support the growth of disc cells, and maintain adhesion to tissue under physiological strain levels. We hypothesized that a genipin crosslinked fi brin gel could meet these requirements. Our mechanical results showed that genipin crosslinked fi brin gels could be created with a modulus in the range of native annular tissue. We also demonstrated that this material is compatible with the in vitro growth of human disc cells, when genipin:fi brin ratios were 0.25:1 or less, although cell proliferation was slower and cell morphology more rounded than for fi brin alone. Finally, lap tests were performed to evaluate adhesion between fi brin gels and pieces of annular tissue. Specimens created without genipin had poor handling properties and readily delaminated, while genipin crosslinked fi brin gels remained adhered to the tissue pieces at strains exceeding physiological levels and failed at 15-30%. This study demonstrated that genipin crosslinked fi brin gels show promise as a gap-fi lling adhesive biomaterial with tunable material properties, yet the slow cell proliferation suggests this biomaterial may be best suited as a sealant for small annulus fi brosus defects or as an adhesive to augment large annulus repairs. Future studies will evaluate degradation rate, fatigue behaviors, and long-term biocompatibility.

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Integrative Repair of Cartilage with Articular and Nonarticular Chondrocytes

Cited by 68 publications

References 27 publications

Implant‐assisted meniscal repair in vivo using a chondrocyte‐seeded flexible PLGA scaffold

Implant‐assisted meniscal repair in vivo using a chondrocyte‐seeded flexible PLGA scaffold

Effects of microcurrent stimulation on Hyaline cartilage repair in immature male rats (Rattus norvegicus)

Genipin-crosslinked fibrin hydrogels as a potential adhesive to augment intervertebral disc annulus repair

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