Implant‐assisted meniscal repair <i>in vivo</i> using a chondrocyte‐seeded flexible PLGA scaffold

Yoo, Jeong Joon; Bichara, David A.; Zhao, Xing; Randolph, Mark A.; Gill, Thomas J.

doi:10.1002/jbm.a.33168

Cited by 21 publications

(24 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[47] Cells were grown in a T-75 culture flask with media as previously described. [48] Briefly, chondrocytes were grown in growth medium (high glucose DMEM supplemented with ITS+ Premix 1% v/v (BD Biosciences), 50 mg/mL L-ascorbic acid 2-phosphate, 40 μg/mL L-proline, 0.1 μM dexamethasone, 110 μg/mL pyruvate, and 1% penicillin-streptomycin-fungizone) with the addition of 10 ng/mL IGF-1 (Peprotech) to maintain cells in a de-differentiated state.…”

Section: Methodsmentioning

confidence: 99%

Development of a Cellularly Degradable PEG Hydrogel to Promote Articular Cartilage Extracellular Matrix Deposition

Sridhar

Brock

Silver

et al. 2015

Adv Healthcare Materials

Self Cite

147

104

View full text Add to dashboard Cite

Healing articular cartilage remains a significant clinical challenge because of its limited self-healing capacity. While delivery of autologous chondrocytes to cartilage defects has received growing interest, combining cell-based therapies with scaffolds that capture aspects of native tissue and promote cell-mediated remodeling could improve outcomes. Currently, scaffold-based therapies with encapsulated chondrocytes permit matrix production; however, resorption of the scaffold does not match the rate of production by cells leading to generally low ECM outputs. Here, a PEG norbornene hydrogel was functionalized with thiolated TGF-β1 and crosslinked by an MMP-degradable peptide. Chondrocytes were co-encapsulated with a smaller population of MSCs, with the goal of stimulating matrix production and increasing bulk mechanical properties of the scaffold. Interestingly, the co-encapsulated cells cleaved the MMP-degradable target sequence more readily than either cell population alone. Relative to non-degradable gels, cellularly-degraded materials showed significantly increased GAG and collagen deposition over just 14 days of culture, while maintaining high levels of viability and producing a more diffuse matrix. These results indicate the potential of an enzymatically-degradable, peptide-functionalized PEG hydrogel to locally influence and promote cartilage matrix production over a short period. Scaffolds that permit cell-mediated remodeling may be useful in designing treatment options for cartilage tissue engineering applications.

show abstract

Section: Methodsmentioning

confidence: 99%

Development of a Cellularly Degradable PEG Hydrogel to Promote Articular Cartilage Extracellular Matrix Deposition

Sridhar

Brock

Silver

et al. 2015

Adv Healthcare Materials

Self Cite

147

104

View full text Add to dashboard Cite

show abstract

“…The meniscus is vascularized only in the outer one‐third (Arnoczky and Warren, ); lesions in the non‐vascularized inner two‐thirds heal poorly or not at all, and lead to joint surface degeneration (Henning et al , ; LeRoux et al , ). Tissue‐engineering techniques could provide an alternative approach to achieving the repair and regeneration of lesions in the avascular meniscus by delivering cells directly to the injury site (Pereira et al , ; Peretti et al , ; Weinand et al , , ; Yoo et al , ; Zellner et al , ).…”

Section: Introductionmentioning

confidence: 99%

Meniscal repairin vivousing human chondrocyte-seeded PLGA mesh scaffold pretreated with platelet-rich plasma

Kwak

Nam

Lee

et al. 2014

J Tissue Eng Regen Med

Self Cite

View full text Add to dashboard Cite

The objective of this study was to test the hypothesis that platelet-rich plasma (PRP) pretreatment on a poly-lactic-co-glycolic acid (PLGA) mesh scaffold enhances the healing capacity of the meniscus with human chondrocyte-seeded scaffolds in vivo, even when the seeded number of cells was reduced from 10 million to one million. A flexible PLGA mesh scaffold was pretreated with PRP using a centrifugal technique. One million human articular chondrocytes were seeded onto the scaffold by dynamic oscillation. After 7 days, scaffolds were placed between human meniscal discs and were implanted subcutaneously in nude mice for 6 weeks (n = 16/group). Fluorescence microscopy demonstrated uniform attachment of the chondrocytes throughout the scaffolds 24 h following seeding. Cell attachment analysis revealed a significantly increased number of chondrocytes on PRP-pretreated than non-treated scaffolds (p < 0.05). Field emission scanning electron microscopy revealed chondrocytes attached to the PRP-pretreated scaffolds interconnecting their cellular processes with the fibrin network at 24 h and day 7 of culture. Of the 16 constructs containing PRP-pretreated scaffolds implanted in mice, six menisci healed completely, nine healed incompletely and one did not heal. Histological results from the 16 control constructs containing non-treated scaffolds revealed that none had healed completely, four healed incompletely and 12 did not heal. The histological outcome between the groups was significantly different (p < 0.05). These findings suggest that human articular chondrocytes on PRP-pretreated PLGA mesh scaffolds demonstrate increased cell attachment and enhance the healing capacity of meniscus with a reduced number of seeding cells in a meniscal repair mouse model. Copyright © 2014 John Wiley & Sons, Ltd.

show abstract

“…Primary chondrocytes were isolated from articular cartilage of the femoral-patellar groove of 6-month old Yorkshire swine as detailed previously [37]. Cells were grown in a T-75 culture flask with media as previously described [38], and were used directly after expansion, P0, for all cell-based experiments.…”

Section: Methodsmentioning

confidence: 99%

A Biosynthetic Scaffold that Facilitates Chondrocyte-Mediated Degradation and Promotes Articular Cartilage Extracellular Matrix Deposition

Sridhar

Dailing

Brock

et al. 2015

Regen. Eng. Transl. Med.

Self Cite

View full text Add to dashboard Cite

Articular cartilage remains a significant clinical challenge to repair because of its limited self-healing capacity. Interest has grown in the delivery of autologous chondrocytes to cartilage defects, and combining cell-based therapies with scaffolds that capture aspects of native tissue and allow cell-mediated remodeling could improve outcomes. Currently, scaffold-based therapies with encapsulated chondrocytes permit matrix production; however, resorption of the scaffold often does not match the rate of matrix production by chondrocytes, which can limit functional tissue regeneration. Here, we designed a hybrid biosynthetic system consisting of poly (ethylene glycol) (PEG) endcapped with thiols and crosslinked by norbornene-functionalized gelatin via a thiol-ene photopolymerization. The protein crosslinker was selected to facilitate chondrocyte-mediated scaffold remodeling and matrix deposition. Gelatin was functionalized with norbornene to varying degrees (~4–17 norbornenes/gelatin), and the shear modulus of the resulting hydrogels was characterized (<0.1–0.5 kPa). Degradation of the crosslinked PEG-gelatin hydrogels by chondrocyte-secreted enzymes was confirmed by gel permeation chromatography. Finally, chondrocytes encapsulated in these biosynthetic scaffolds showed significantly increased glycosaminoglycan deposition over just 14 days of culture, while maintaining high levels of viability and producing a distributed matrix. These results indicate the potential of a hybrid PEG-gelatin hydrogel to permit chondrocyte-mediated remodeling and promote articular cartilage matrix production. Tunable scaffolds that can easily permit chondrocyte-mediated remodeling may be useful in designing treatment options for cartilage tissue engineering applications.

show abstract

Implant‐assisted meniscal repair in vivo using a chondrocyte‐seeded flexible PLGA scaffold

Cited by 21 publications

References 36 publications

Development of a Cellularly Degradable PEG Hydrogel to Promote Articular Cartilage Extracellular Matrix Deposition

Development of a Cellularly Degradable PEG Hydrogel to Promote Articular Cartilage Extracellular Matrix Deposition

Meniscal repairin vivousing human chondrocyte-seeded PLGA mesh scaffold pretreated with platelet-rich plasma

A Biosynthetic Scaffold that Facilitates Chondrocyte-Mediated Degradation and Promotes Articular Cartilage Extracellular Matrix Deposition

Contact Info

Product

Resources

About