Integration of Pillar Array Columns into a Gradient Elution System for Pressure-Driven Liquid Chromatography

Song, Yanting; Noguchi, Masao; Takatsuki, Katsuya; Sekiguchi, Tetsushi; Mizuno, Jun; Funatsu, Takashi; Shoji, Shuichi; Tsunoda, Makoto

doi:10.1021/ac3001836

Cited by 43 publications

(46 citation statements)

References 20 publications

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“…The microchip fabrication has been described in previous studies [7][8][9][10]. The 20 mm × 20 mm microchip was composed of a cross-Tesla mixer, a separation channel, and a sample channel.…”

Section: Fabrication Of the Microchipmentioning

confidence: 99%

“…Based on this assumption, a practical equation (Eq. (9)) was utilized for the prediction of analyte peak widths under gradient elution conditions: (9) where w g is the peak width under gradient elution conditions. N* cannot be determined directly from the data because the retention factor k constantly changes during gradient elution.…”

Section: Estimation Of Peak Widths Of Nbd-aliphatic Amines Under Gradmentioning

confidence: 99%

“…Four NBD-aliphatic amines were analyzed under gradient elution conditions with gradient times of 90 and 450 s [9]. Additionally, during this study, additional analyses were performed with gradient times of 150, 300, 360, and 600 s. As shown in Fig.…”

Section: Gradient Elution Of Nbd-aliphatic Aminesmentioning

confidence: 99%

“…(9). Then, with gradient times of 90, 150, 300, 360, and 600 s, the peak widths of NBD-aliphatic amines were estimated and listed in Table 3.…”

Section: Estimation Of Peak Widths Of Nbd-aliphatic Amines Under Gradmentioning

confidence: 99%

“…Six fluorescently labeled amino acids were analyzed within 100 s by isocratic elution using the pillar array column [7,8]. Furthermore, to separate the components of complex biological samples, a gradient elution system was developed to accelerate the elution of strongly retained solutes using pressure-driven liquid chromatography (LC) on a microchip [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column

et al. 2016

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Previously, we have developed a gradient elution system for pillar array columns, which achieved faster separation than isocratic elution. In this study, we validated gradient elution in microchip liquid chromatography (LC) and investigated the retention and bandwidth predictions of fluorescently labeled aliphatic amines in fast gradient elution chromatography using semi-empirical retention models. The retention times and peak widths under different gradient elution programs were predicted by three solvent strength models and compared with the experimental results. The relative errors of prediction for the retention times and peak widths were below 14 % and 12 %, respectively. The results showed that the solvent strength models could be utilized for predicting the retention times and peak widths under gradient elution in the microchip LC system and that the gradient elution program for pillar array columns worked efficiently. The prediction by the retention model promises to be a potential tool for essential compound identification in biological samples.

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Section: Fabrication Of the Microchipmentioning

confidence: 99%

Section: Estimation Of Peak Widths Of Nbd-aliphatic Amines Under Gradmentioning

confidence: 99%

Section: Gradient Elution Of Nbd-aliphatic Aminesmentioning

confidence: 99%

“…(9). Then, with gradient times of 90, 150, 300, 360, and 600 s, the peak widths of NBD-aliphatic amines were estimated and listed in Table 3.…”

Section: Estimation Of Peak Widths Of Nbd-aliphatic Amines Under Gradmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column

et al. 2016

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The Effective Transmissivity of a Plane‐Walled Fracture With Circular Cylindrical Obstacles

Jasinski

Dąbrowski

2018

JGR Solid Earth

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Stimulated and propped fractures provide the conductive pathways in low matrix permeability rocks. We study the impact of fracture aperture and proppant size and fraction on the effective transmissivity of a stimulated fracture filled with a partial monolayer of proppant grains. The proppant grains are treated as circular cylindrical obstacles, and the fracture walls are planar. The key geometric parameters are the obstacle fraction f and the ratio between the fracture aperture and the obstacle diameter α. We use three‐dimensional Stokes flow numerical simulations to demonstrate that the fracture flow model given by the Reynolds equations may largely overestimate the flow rate. To circumvent the inability of the Reynolds model to fulfill the no‐slip boundary condition at the rims of the obstacles, we use the Brinkman flow model adapted to the case of fracture flow. The relative difference between the effective transmissivities computed with the Stokes and Brinkman models for systems with multiple obstacles is below 10% for fractions up to 0.5. We use the Brinkman model to study the effective transmissivity of a plane‐walled fracture with circular cylindrical obstacles. Systematic numerical simulations show that the normalized effective transmissivity is predominantly dependent on f and α, and the effects of obstacle ordering are minor. The presented numerical results can be used by petroleum engineers for estimating transmissivities of propped fractures under in situ conditions. The model can also be applied to microfluidic systems and for deriving first‐order estimates of the effective transmissivity of rough‐walled, natural fractures with load‐bearing contact areas.

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Performance of laterally elongated pillar array columns in capillary electrochromatography mode

et al. 2020

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Integration of Pillar Array Columns into a Gradient Elution System for Pressure-Driven Liquid Chromatography

Cited by 43 publications

References 20 publications

Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column

Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column

The Effective Transmissivity of a Plane‐Walled Fracture With Circular Cylindrical Obstacles

Performance of laterally elongated pillar array columns in capillary electrochromatography mode

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