Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column

Song, Yanting; Takatsuki, Katsuya; Sekiguchi, Tetsushi; Funatsu, Takashi; Shoji, Shuichi; Tsunoda, Makoto

doi:10.15583/jpchrom.2016.008

Cited by 6 publications

(6 citation statements)

References 27 publications

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“…Then, as a third axis, the u0 of each point ΔP and t0 was calculated using Eqs. (1) and (4). N was calculated using u0 with Eqs.…”

Section: A Precise Method Based On the Kpl For Determining Column Cmentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8][9][10][11] However, it is difficult to select the optimal packing support, and to find suitable separation conditions, given a specific pressure in an HPLC system; and peripheral studies on the theory have thus far been limited. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] In 2005, Desmet et al invented kinetic performance methods 28 by expanding both Bristow and Knox's, 29 and Poppe's 30 methods.…”

Section: Introductionmentioning

confidence: 99%

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Three-dimensional Representation Method Using Pressure, Time, and Number of Theoretical Plates to Analyze Separation Conditions in HPLC Columns

2018

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There has been considerable discussion of the speed performance of HPLC separation, especially regarding the relationship between theoretical plates and hold-up time. The fundamental discussion focuses on the optimal velocity, u0,opt, which gives a minimal height equivalent to a theoretical plate of the van Deemter plot. On the other hand, Desmet's method, using the kinetic performance limit (KPL), calculates the highest performance with a constant pressure drop, without focusing solely on the optimal velocity. In this paper, a precise method based on the KPL is proposed, to understand how increasing pressure enhances both theoretical plates and hold-up time. A three-dimensional representation method that combines the pressure drop with two axes of time and theoretical plates will be useful for discussing the effect of pressure in pressure-driven chromatography. Using three dimensions, the methods based on u0,opt and the KPL can be combined, because u0,opt can be visualized three-dimensionally, including the neighbor of u0,opt; and the question of whether the KPL is an asymptotic or effective limit can be investigated. Three performances of high resolution, high speed, and low pressure can be understood on different packing supports at a glance.

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“…Then, as a third axis, the u0 of each point ΔP and t0 was calculated using Eqs. (1) and (4). N was calculated using u0 with Eqs.…”

Section: A Precise Method Based On the Kpl For Determining Column Cmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Three-dimensional Representation Method Using Pressure, Time, and Number of Theoretical Plates to Analyze Separation Conditions in HPLC Columns

2018

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“…To overcome the problem of nonuniformity in the internal structure of particle-packed columns, pillar array columns with a completely uniform internal structure have recently been developed [8][9][10][11][12][13]. The use of semiconductor microfabrication technology enables the uniform arrangement of pillars of the same size in a silicon substrate.…”

Section: Introductionmentioning

confidence: 99%

Development of an Automated Sample Injection System for Pillar Array Columns

et al. 2020

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A pillar array column with a perfectly ordered internal structure has a higher separation efficiency than a particle-packed column used in conventional high-performance liquid chromatography. However, applying the pillar array column technology to quantitative analysis is challenging as the injection volume of the sample varies for each injection. This occurs because the sample volumes are in the order of nanoliters and the sample is injected manually. In this study, an automated sample injection system was developed to solve this problem. The system was composed of two pumps (one for the sample and other for the mobile phase), a six-way valve that can be controlled by a PC, and an autosampler. The peak height deviation, which is more than 20% in the conventional manual injection system, was improved to 1.2% and 0.4% for the two coumarin dye samples. This result indicated that the automated sample injection method developed in this study could be applied to pillar array columns to allow for quantitative analysis.

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“…Pillar array columns with perfectly ordered structures can shorten separation times significantly without lessening the quality of the separation. [9][10][11][12][13][14][15][16] However, pillar array columns filled with non-porous pillars have limited retention ability since only the outer surface of the pillars is available for separation. Therefore, until now, only fluorescent derivatives of hydrophobic amino acids, such as valine (Val), phenylalanine, and tyrosine, derivatized with the flurogenic reagent, 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), were retained and separated by pillar array columns.…”

Section: Introductionmentioning

confidence: 99%

Retention of Fluorescent Amino Acid Derivatives in Ion-pairing Reversed-phase Liquid Chromatography

Kuroki

Funatsu

et al. 2018

ANAL. SCI.

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Recent studies have shown that pillar array columns enable fast and quantitative analysis of amino acids. However, hydrophilic amino acids still cannot be retained on pillar array columns since they have limited retention ability. Ionpairing liquid chromatography is a promising means of increasing analyte retention. In this study, the effects of ionpairing reagents on the retention of eight hydrophilic amino acids (histidine, asparagine, glutamine, serine, arginine, aspartic acid, glycine, and glutamic acid) derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) under reversedphase conditions on a conventional ODS column were studied. Among the ion-pairing reagents investigated, tetraheptylammonium bromide proved to be the most effective for increasing analyte retention. With a mobile phase consisting of 20 mM citrate buffer (pH 6.3)-acetonitrile (100:40, v/v) and 2 mM tetraheptylammonium bromide, the retention times of the eight NBD-amino acids-except NBD-arginine-were longer than 19.4 min, which was the retention time of NBD-valine when eluted without an ion-pairing reagent. As NBD-valine was well retained on pillar array columns, the chromatographic conditions may thus be applied in the analysis of hydrophilic amino acids using pillar array columns.

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Retention and Bandwidth Predictions by Fast Gradient Elution Chromatography Using a Pillar Array Column

Cited by 6 publications

References 27 publications

Three-dimensional Representation Method Using Pressure, Time, and Number of Theoretical Plates to Analyze Separation Conditions in HPLC Columns

Three-dimensional Representation Method Using Pressure, Time, and Number of Theoretical Plates to Analyze Separation Conditions in HPLC Columns

Development of an Automated Sample Injection System for Pillar Array Columns

Retention of Fluorescent Amino Acid Derivatives in Ion-pairing Reversed-phase Liquid Chromatography

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