Integrated platform with magnetic purification and rolling circular amplification for sensitive fluorescent detection of ochratoxin A

Li, Yao; Yinji, Chen; Teng, Jun; Zheng, Wanli; Wu, Jingjing; Adeloju, Samuel B O; Pan, Daodong; Chen, Wei

doi:10.1016/j.bios.2015.06.056

Cited by 34 publications

(16 citation statements)

References 37 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Using an optimal RCA template evolved from in vitro selection, the detection sensitivity of a reduced graphene oxide‐based method can be significantly improved . In contrast with aptamer recognition‐induced primer immobilization or release, some researchers made use of aptamer‐target recognition to inhibit the functions of primers, and then impede RCA reaction, leading to a decrease in scanometric or fluorescent intensities . Aptamer recognition‐induced release of primer for RCA assay is relatively facile and can be easily coupled with other methods for target detection.…”

Section: Rca With Functional Nucleic Acidsmentioning

confidence: 99%

Circular Nucleic Acids: Discovery, Functions and Applications

Mohammed-Elsabagh

Paczkowski

et al. 2020

ChemBioChem

View full text Add to dashboard Cite

acids (CNAs) are nucleic acid molecules with a closed-loop structure. This feature comes with a number of advantages including complete resistance to exonuclease degradation, much better thermodynamic stability, and the capability of being replicated by a DNA polymerase in a rolling circle manner. Circular functional nucleic acids, CNAs containing at least a ribozyme/DNAzyme or a DNA/RNA aptamer, not only inherit the advantages of CNAs but also offer some unique application opportunities, such as the design of topologycontrolled or enabled molecular devices. This article will begin by summarizing the discovery, biogenesis, and applications of naturally occurring CNAs, followed by discussing the methods for constructing artificial CNAs. The exploitation of circular functional nucleic acids for applications in nanodevice engineering, biosensing, and drug delivery will be reviewed next. Finally, the efforts to couple functional nucleic acids with rolling circle amplification for ultra-sensitive biosensing and for synthesizing multivalent molecular scaffolds for unique applications in biosensing and drug delivery will be recapitulated.[a] Dr.

show abstract

Section: Rca With Functional Nucleic Acidsmentioning

confidence: 99%

Circular Nucleic Acids: Discovery, Functions and Applications

Mohammed-Elsabagh

Paczkowski

et al. 2020

ChemBioChem

View full text Add to dashboard Cite

show abstract

“…Since the magnetic carriers have got functional groups like ion exchanger ligands, hydrophobic groups or bio-polymeric agents, the purification can be done in one step without utilizing centrifuges, filters or other equipment 36 . For this purpose, carboxyl-MNPs were prepared by solve-thermal protocol 37 and were surface modified via the RCA primer containing the specific aptamer related to OTA 38 .…”

Section: Detection Of Ochratoxin a By Combining Magnetic Nanoparticlementioning

confidence: 99%

Different Applications of Magnetic Nanoparticles in the Rapid Monitoring of Ochratoxin A

Sargazi¹,

Majd²

2017

Orient. J. Chem

View full text Add to dashboard Cite

Toxic effects of mycotoxins are still an unresolved subject for health of humans and animals. Therefore, several methods has been created to determine and eliminate mycotoxins from foodstuffs, and also are developing. For instance, utilization of magnetic nanoparticles (MNPs) when are combined with different analytical tools is one of the developing methods which could be useful for extraction of mycotoxins. As in some methods, they were employed as newfound adsorbents in magnetic solid phase extraction approaches. In some other methods, MNPs were used for immobilization of antibodies to enhance the efficiency of the analytical tools such as square wave voltammetry. Or in another method, amine-functionalized MNPs were reacted with ochratoxin A (OTA) to enhance performance of chemiluminescence immunoassay. Results of all methods were showed that utilizing of MNPs could reduce cost, easy preparation, and increase the stability, speed, sensitivity, precision and reproducibility. So, these new methods have elicited great hopes to be suitable alternative for older methods.

show abstract

“…In the presence of OTA, fluorescent particles would be partially released due to the competitive binding between OTA and specific OTA aptamer. For wider linear range and higher sensitivity, Yao et al [89] developed a biosensor for the OTA measurement integrating magnetic nanoparticles with rolling circular amplification (RCA). Magnetic nanoparticles were used to decrease the signal via efficient separation, and RCA was used for the signal amplification.…”

Section: Applicationsmentioning

confidence: 99%

Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

Zhang

et al. 2016

Toxins

View full text Add to dashboard Cite

Mycotoxin contamination threatens health and life of humans and animals throughout the food supply chains. Many of the mycotoxins have been proven to be carcinogens, teratogens and mutagens. The reliable and sensitive sensing methods are requested to monitor mycotoxin contamination. Advanced sensors based on antibodies or aptamers boast the advantages of high sensitivity and rapidity, and have been used in the mycotoxin sensing. These sensors are miniaturized, thereby lowering costs, and are applicable to high-throughput modes. In this work, the latest developments in sensing strategies for mycotoxin determination were critically discussed. Optical and electrochemical sensing modes were compared. The sensing methods for single mycotoxin or multiple mycotoxins in food samples were reviewed, along with the challenges and the future of antibody or aptamer-based sensors. This work might promote academic studies and industrial applications for mycotoxin sensing.

show abstract

Integrated platform with magnetic purification and rolling circular amplification for sensitive fluorescent detection of ochratoxin A

Cited by 34 publications

References 37 publications

Circular Nucleic Acids: Discovery, Functions and Applications

Circular Nucleic Acids: Discovery, Functions and Applications

Different Applications of Magnetic Nanoparticles in the Rapid Monitoring of Ochratoxin A

Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

Contact Info

Product

Resources

About