Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

Xu, Lin; Zhang, Zhaowei; Zhang, Qi; Li, Peiwu

doi:10.3390/toxins8080239

Cited by 50 publications

(33 citation statements)

References 103 publications

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“…Among LFIAs, LFIAs with competitive format (cLFIA) are commonly used to detect small chemical molecules (hapten), such as veterinary, illegal drugs, antibiotics, and mycotoxins. However, the cLFIA frequently suffers from relatively low sensitivity because it requires a large amount of target analytes to eliminate the signals on the test (T) line (signal “turn off”) by competitively binding the antibody on the probe [6]. Recently, some research groups reported a novel fluorescence-quenching LFIA sensor in improving the sensitivity of the cLFIA method based on the signal “turn-on” mode.…”

Section: Introductionmentioning

confidence: 99%

Silver Nanoparticle-Based Fluorescence-Quenching Lateral Flow Immunoassay for Sensitive Detection of Ochratoxin A in Grape Juice and Wine

Xiong

et al. 2017

Toxins

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A silver nanoparticle (AgNP)-based fluorescence-quenching lateral flow immunoassay with competitive format (cLFIA) was developed for sensitive detection of ochratoxin A (OTA) in grape juice and wine samples in the present study. The Ru(phen)32+-doped silica nanoparticles (RuNPs) were sprayed on the test and control line zones as background fluorescence signals. The AgNPs were designed as the fluorescence quenchers of RuNPs because they can block the exciting light transferring to the RuNP molecules. The proposed method exhibited high sensitivity for OTA detection, with a detection limit of 0.06 µg/L under optimized conditions. The method also exhibited a good linear range for OTA quantitative analysis from 0.08 µg/L to 5.0 µg/L. The reliability of the fluorescence-quenching cLFIA method was evaluated through analysis of the OTA-spiked red grape wine and juice samples. The average recoveries ranged from 88.0% to 110.0% in red grape wine and from 92.0% to 110.0% in grape juice. Meanwhile, less than a 10% coefficient variation indicated an acceptable precision of the cLFIA method. In summary, the new AgNP-based fluorescence-quenching cLFIA is a simple, rapid, sensitive, and accurate method for quantitative detection of OTA in grape juice and wine or other foodstuffs.

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Section: Introductionmentioning

confidence: 99%

Silver Nanoparticle-Based Fluorescence-Quenching Lateral Flow Immunoassay for Sensitive Detection of Ochratoxin A in Grape Juice and Wine

Xiong

et al. 2017

Toxins

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“…Due to its prevalence and importance, several analytical methods have been developed and extensively reviewed for the quantitative determination of OTA in foodstuffs [6,7,8,9]. These methods, which allow the detection of OTA alone or in combination with other mycotoxins, are mainly based on high-performance liquid chromatography (HPLC) coupled to fluorescence or mass spectrometry detection.…”

Section: Introductionmentioning

confidence: 99%

Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

McKeague

Velu

Girolamo

et al. 2016

Toxins

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Ochratoxin A (OTA) is a mycotoxin produced as a secondary metabolite by several species of Aspergillus and Penicillium and frequently found as a natural contaminant in a wide range of food commodities. Novel and robust biorecognition agents for detecting this molecule are required. Aptamers are artificial nucleic acid ligands able to bind with high affinity and specificity to a given target molecule. In the last few years, three separate research groups have selected aptamers for ochratoxin A. While each of these three families of aptamers have been incorporated into various methods for detecting OTA, it is unclear if each aptamer candidate is better suited for a particular application. Here, we perform the first head-to-head comparison of solution-based binding parameters for these groups of aptamers. Based on our results, we provide recommendations for the appropriate choice of aptamer for incorporation into solution-based biorecognition assays and applications.

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“…Moving on, the co‐contamination of food has long been one of the key concerns in food safety. Coupled with the possibility of simultaneous contamination by different fungal species, ensuring the selectivity of any biosensor for the detection of mycotoxins becomes crucial. To this aim, fumonisin B 1 (FB1) was selected as the negative control as the co‐occurrence of OTA and fumonisin B 1 in food has been reported and both molecules are of similar sizes; at the same time thrombin (THR) was also chosen as negative control, being an example of another analyte that can be successfully detected by using DNA aptamers as the biorecognition element .…”

Section: Resultsmentioning

confidence: 99%

The Role of Surface Chemistry in Impedimetric Aptasensing

2018

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Surface chemistry is a key parameter in the choice of proper materials for electrochemical detection. It has been previously shown that the presence of oxygen containing groups (OCGs) on the surface of graphene oxide (GO) can be both effective and detrimental. This poses a question when GO materials are used as electrochemical platforms for biosensing. In this work, we study how the surface chemistry of graphene oxide nanocolloids (GONCs) affects the impedimetric biosensing of ochratoxin A (OTA), in terms of immobilization of biorecognition element and detection step. OCGs on GONCs were tuned by applying increasing reduction potentials from −0.3 V to −1.2 V, resulting in GONC platforms with decreasing amounts of oxygen functionalities. It was discovered that the sensitivity of biosensing is correlated to the residual amount of OCGs on GO surface. For a more detailed investigation, three representative materials, namely unreduced GONCs, as well as GONCs reduced at potentials of −0.8 V and −1.2 V were chosen. Results were compared in terms of calibration sensitivity, selectivity and reproducibility of the impedimetric response. GONCs reduced at −1.2 V have shown the best electroanalytical response for the impedimetric detection of OTA. These findings are anticipated to contribute to the design of novel biosensors, whereby an optimized platform is employed for the immobilization of the biorecognition element.

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Mycotoxin Determination in Foods Using Advanced Sensors Based on Antibodies or Aptamers

Cited by 50 publications

References 103 publications

Silver Nanoparticle-Based Fluorescence-Quenching Lateral Flow Immunoassay for Sensitive Detection of Ochratoxin A in Grape Juice and Wine

Silver Nanoparticle-Based Fluorescence-Quenching Lateral Flow Immunoassay for Sensitive Detection of Ochratoxin A in Grape Juice and Wine

Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A

The Role of Surface Chemistry in Impedimetric Aptasensing

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