Integrated microfluidic capillary in a waveguide resonator for chemical and biomedical sensing

Pavuluri, Sumanth Kumar; Lopez-Villarroya, R.; McKeever, E.; Goussetis, George; Desmulliez, Marc Phillipe Yves; Kavanagh, Deirdre M.

doi:10.1088/1742-6596/178/1/012009

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Finally, one of the most exciting prospects of FMR based biosensing is the large potential for miniaturization of sensor units. While experiments performed in the current work were carried out on a bulky commercial EPR spectrometer, the sensor unit may be scaled down to length scales relevant for microfluidic systems (e.g., by the use of coplanar waveguides), , or even to the micron-scale using microfabricated resonators. , …”

Section: Resultsmentioning

confidence: 99%

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

et al. 2018

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The ability to detect and analyze the state of magnetic labels with high sensitivity is of crucial importance for developing magnetic biosensors. In this work, we demonstrate, for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, i.e., rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP), are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For the RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with a linear detection range between 7.8 and 250 pM. For the LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their coprecipitation with MgPO (a byproduct of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity, and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.

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Section: Resultsmentioning

confidence: 99%

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

et al. 2018

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“…While experiments performed in the current work were carried out on a bulky commercial EPR spectrometer, the sensor unit may be scaled down to length scales relevant for microfluidic systems (e.g. by the use of co-planar waveguides), 46 or even to the micron-scale using microfabricated resonators. 47,48 Working principle of LAMP-based FMR sensing.…”

Section: Rca-based Dna Quantification In Order To Test the Capability Of The Fmr Technique To Probementioning

confidence: 99%

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

Tian

Svedlindh²,

Strömberg³

et al. 2017

Preprint

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In this work, we demonstrate for the first time, a ferromagnetic resonance (FMR) based homogeneous and volumetric biosensor for magnetic label detection. Two different isothermal amplification methods, i.e., rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) are adopted and combined with a standard electron paramagnetic resonance (EPR) spectrometer for FMR biosensing. For RCA-based FMR biosensor, binding of RCA products of a synthetic Vibrio cholerae target DNA sequence gives rise to the formation of aggregates of magnetic nanoparticles. Immobilization of nanoparticles within the aggregates leads to a decrease of the net anisotropy of the system and a concomitant increase of the resonance field. A limit of detection of 1 pM is obtained with an average coefficient of variation of 0.16%, which is superior to the performance of other reported RCA-based magnetic biosensors. For LAMP-based sensing, a synthetic Zika virus target oligonucleotide is amplified and detected in 20% serum samples. Immobilization of magnetic nanoparticles is induced by their co-precipitation with Mg2P2O7 (a by-product of LAMP) and provides a detection sensitivity of 100 aM. The fast measurement, high sensitivity and miniaturization potential of the proposed FMR biosensing technology makes it a promising candidate for designing future point-of-care devices.

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An Inkjet-Printed Microfluidic RFID-Enabled Platform for Wireless Lab-on-Chip Applications

Cook

Cooper

Tentzeris

2013

IEEE Trans. Microwave Theory Techn.

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This paper introduces the first-of-its-kind wireless passive sensing platform combining radio frequency identification (RFID), microfluidics, and inkjet printing technology that enables remote fluid analysis and requires as little as 3 L of fluid. The demonstrated variable microfluidic capacitors, resonators, and RFID tags are fabricated using a novel rapid, low-cost, and low-temperature additive inkjet process, making them disposable. However, even with their disposable nature, the RF microfluidic devices exhibit repeatability and long-term reusability for accurately detecting water, various alcohols, and % content of water/alcohol mixtures down to 1% water in ethanol. While the main discussion is on fluid sensing, the demonstrated components can also be used in fluid-tunable RF applications.

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Integrated microfluidic capillary in a waveguide resonator for chemical and biomedical sensing

Cited by 3 publications

References 10 publications

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

Ferromagnetic Resonance Biosensor for Homogeneous and Volumetric Detection of DNA

An Inkjet-Printed Microfluidic RFID-Enabled Platform for Wireless Lab-on-Chip Applications

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