Insulin‐like growth factor I messenger RNA and protein are expressed in the human lymph node and distinctly confined to subtypes of macrophages, antigen‐presenting cells, lymphocytes and endothelial cells

Oberlin, Dominique; Fellbaum, Christian; Eppler, Elisabeth

doi:10.1111/j.1365-2567.2009.03136.x

Cited by 19 publications

(19 citation statements)

References 62 publications

(72 reference statements)

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“…7a) or in CD3+ T lymphocytes (data not shown). The absence of IGF-I in lymphocytes of the human lymph node is similar to our previous findings in CD3+ T lymphocytes and CD20+ B lymphocytes in the non-malignant lymph node (Oberlin et al 2009;present study). The absence of IGF-I in non-reactive B cells in our study is also in accordance with observations by other groups that freshly isolated B lymphocytes do not secrete IGF-I in vitro (Merrimee et al 1989).…”

Section: Fig 5 Igf-i In Cd68+supporting

confidence: 90%

“…In the present study, we detected IGF-I-immunoreactive material in macrophages and high endothelial venules of HL lesions, as previously described in non-tumoral lymph nodes in which we proposed a role for IGF-I in the differentiation of the special endothelium and attraction of lymphocytes and, particularly, in the support of macrophages during immune challenge (Oberlin et al 2009). Thus, IGF-I might contribute to the constitution and maintenance of the tumour microenvironment, in addition to its local presence in the tumour cells themselves.…”

Section: Fig 5 Igf-i In Cd68+supporting

confidence: 69%

“…Subsequently, the antibody against CD30 was applied overnight at 4°C and visualised with Texas-red-conjugated goat anti-mouse IgG. Specificity of the antisera was tested by omitting the primary antisera as a negative control and by using sections of human breast cancer, intestine and lymph node and rat pancreas as positive controls as previously described (Eppler et al 2002;Jevdjovic et al 2004Jevdjovic et al , 2005Oberlin et al 2009;Link et al 2013) and an embryonic tumour known to contain CD30-immunoreactive cells.…”

Section: Double-immunofluorescencementioning

confidence: 99%

“…In brief, for demasking of the antigens, sections were treated with antigen-retrieval solution according to the recommendations of the manufacturer (DAKO); they were then blocked with 2 % bovine serum albumin in phosphate-buffered saline (pH 7.4) and incubated overnight at 4°C with rabbit IGF-I antiserum (Oberlin et al 2009) followed by visualisation with fluorescein isothiocyanate (FITC)-coupled goat anti-rabbit IgG (Bioscience Products, Emmenbrücke, Switzerland). Subsequently, antibodies against CD3 (Abcam, UK), CD15, CD20, CD30 and CD68 (all from DAKO) were applied overnight at 4°C and visualised with Texas-redconjugated goat anti-mouse IgGs (Bioscience and Amersham, Little Chalfont, UK).…”

Section: Double-immunofluorescencementioning

confidence: 99%

“…Double-immunofluorescence for IGF-I with CD markers was performed as previously established for human lymph nodes (Oberlin et al 2009) with the modification that for cHL CD30, for NLPHL CD20, and for granulocytes CD15 (all DAKO Cytomation, Glostrup, Denmark) were additionally applied. In brief, for demasking of the antigens, sections were treated with antigen-retrieval solution according to the recommendations of the manufacturer (DAKO); they were then blocked with 2 % bovine serum albumin in phosphate-buffered saline (pH 7.4) and incubated overnight at 4°C with rabbit IGF-I antiserum (Oberlin et al 2009) followed by visualisation with fluorescein isothiocyanate (FITC)-coupled goat anti-rabbit IgG (Bioscience Products, Emmenbrücke, Switzerland).…”

Section: Double-immunofluorescencementioning

confidence: 99%

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Insulin-like growth factor I is expressed in classical and nodular lymphocyte-predominant Hodgkin’s lymphoma tumour and microenvironmental cells

et al. 2014

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Hodgkin's lymphoma (HL) is among the most frequent nodal lymphomas in the Western world and is classified into two disease entities: nodular lymphocyte-predominant Hodgkin's lymphoma (NLPHL) and classical Hodgkin's lymphoma (cHL, 95% of all HL). HL lesions are characterised by a minority of clonal neoplastic cells, namely Hodgkin and Reed-Sternberg (HRS) cells and their variants in cHL and lymphocyte-predominant (LP) cells in NLPHL, both occurring within a microenvironment of, for example, reactive T and B cells, macrophages and granulocytes that are assumed to support the proliferation and maintenance of neoplastic cells through cytokines, chemokines and growth factors. Insulin-like growth factor I (IGF-I) is an important growth factor involved in proliferation, differentiation, apoptosis and cell survival of numerous (including immune) tissues and probably has a role in tumour pathogenesis and maintenance. Although HL is characterised by disturbed cell differentiation and apoptosis mechanisms, with the involvement of the IGF-I receptor (IGF-1R), the distinct location of IGF-I in HL has not yet been defined. We localise IGF-I by double-immunofluorescence in frequent neoplastic cells of all cHL and NLPHL cases investigated. Additionally, IGF-I immunoreactivity is detected in high endothelial venules and various immune cells within the surrounding tissue of cHL including neutrophils and macrophages. IGF-1R immunoreactivity of variable intensity is found in HRS cells and high endothelial venules within the microenvironment in cHL. We assume that autocrine and paracrine IGF-I plays an anti-apoptotic role in tumour pathogenesis and in shaping the tumour microenvironment.

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Section: Fig 5 Igf-i In Cd68+supporting

confidence: 90%

Section: Fig 5 Igf-i In Cd68+supporting

confidence: 69%

Section: Double-immunofluorescencementioning

confidence: 99%

Section: Double-immunofluorescencementioning

confidence: 99%

Section: Double-immunofluorescencementioning

confidence: 99%

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Insulin-like growth factor I is expressed in classical and nodular lymphocyte-predominant Hodgkin’s lymphoma tumour and microenvironmental cells

et al. 2014

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Overload training inhibits phagocytosis and ROS generation of peritoneal macrophages: role of IGF-1 and MGF

et al. 2012

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We tested the hypothesis that overload training inhibits the phagocytosis and the reactive oxygen species (ROS) generation of peritoneal macrophages (Mϕs), and that insulin-like growth factor-1(IGF-1) and mechano-growth factor (MGF) produced by macrophages may contribute to this process. Rats were randomized to two groups, sedentary control group (n = 10) and overload training group (n = 10). The rats of overload training group were subjected to 11 weeks of experimental training protocol. Blood sample was used to determine the content of hemoglobin, testosterone, and corticosterone. The phagocytosis and the ROS generation of Mϕs were measured by the uptake of neutral red and the flow cytometry, respectively. IGF-1 and MGF mRNA levels in Mϕs were determined by real-time PCR. In addition, we evaluated the effects of IGF-1 and MGF peptide on phagocytosis and ROS generation of Mϕs in vitro. The data showed that overload training significantly decreased the body weight (19.3 %, P < 0.01), the hemoglobin (13.5 %, P < 0.01), the testosterone (55.3 %, P < 0.01) and the corticosterone (40.6 %, P < 0.01) in blood. Moreover, overload training significantly decreased the phagocytosis (27 %, P < 0.05) and the ROS generation (35 %, P < 0.01) of Mϕs. IGF-1 and MGF mRNA levels in Mϕs from overload training group increased significantly compared with the control group (21-fold and 92-fold, respectively; P < 0.01). In vitro experiments showed that IGF-1 had no significant effect on the phagocytosis and the ROS generation of Mϕs. Unlike IGF-1, MGF peptide impaired the phagocytosis of Mϕs in dose-independent manner. In addition, MGF peptide of some concentrations (i.e., 1, 10, 50, 100 ng/ml) significantly inhibited the ROS generation of Mϕs. These results suggest that overload training inhibits the phagocytosis and the ROS generation of peritoneal macrophages, and that MGF produced by macrophages may play a key role in this process. This may represent a novel mechanism of immune suppression induced by overload training.

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Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells

et al. 2014

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The adult heart contains a population of cardiac progenitor cells (CPCs). Growing and collecting an adequate number of CPCs demands complex culture media containing growth factors. Since activated macrophages secrete many growth factors, we investigated if activated isolated heart cells seeded on a feeder layer of activated peritoneal macrophages (PM) could result in CPCs and if these, in turn, could exert cardioprotection in rats with myocardial infarction (MI). Heart cells of inbred Wistar rats were isolated by collagenase digestion and cultured on PM obtained 72 h after intraperitoneal injection of 12 ml thioglycollate. Cells (1 × 10(6)) exhibiting CPC phenotype (immunohistochemistry) were injected in the periphery of rat MI 10 min after coronary artery occlusion. Control rats received vehicle. Three weeks later, left ventricular (LV) function (echocardiogram) was assessed, animals were euthanized and the hearts removed for histological studies. Five to six days after seeding heart cells on PM, spherical clusters composed of small bright and spherical cells expressing mostly c-Kit and Sca-1 antigens were apparent. After explant, those clusters developed cobblestone-like monolayers that expressed smooth muscle actin and sarcomeric actin and were successfully transferred for more than ten passages. When injected in the MI periphery, many of them survived at 21 days after coronary ligature, improved LV ejection fraction and decreased scar size as compared with control rats. CPC-derived cells with cardiocyte and smooth muscle phenotypes can be successfully grown on a feeder layer of activated syngeneic PM. These cells decreased scar size and improved heart function in rats with MI.

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Insulin‐like growth factor I messenger RNA and protein are expressed in the human lymph node and distinctly confined to subtypes of macrophages, antigen‐presenting cells, lymphocytes and endothelial cells

Cited by 19 publications

References 62 publications

Insulin-like growth factor I is expressed in classical and nodular lymphocyte-predominant Hodgkin’s lymphoma tumour and microenvironmental cells

Insulin-like growth factor I is expressed in classical and nodular lymphocyte-predominant Hodgkin’s lymphoma tumour and microenvironmental cells

Overload training inhibits phagocytosis and ROS generation of peritoneal macrophages: role of IGF-1 and MGF

Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells

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