Insulin and the Dextran Reaction in the Rat

Abstract

Insulin potentiated the dextran anaphylactoid reaction in untreated rats and in rats pretreated with a protein synthesis inhibitor or with a lymphopenic agent. Incubation of rat lymphatic tissue with insulin failed to produce a factor potentiating the histamine-releasing action of dextran when this was studied in both in vivo and in vitro tests. It thus seems that insulin is exerting its potentiating effect on the dextran response through processes not involving synthesis of new protein or interaction with lymphocytes.

Histamine release from isolated and intact mast cells of two types of genetically different rat

“…Similar results were obtained by other workers [4] in the skin of R rats using dextran as the stimulant of increased vascular permeability. These results suggest that the three agents induce histamine release from isolated peritoneal mast cells by a similar mechanism.…”

“…Care must be exercised in interpreting results using isolated peritoneal mast cells of the rat for events may be different in the whole animal. This result supplements two previous findings -(1) calcium ionophores like A 23187 do not release histamine when injected intradermally yet they are potent releasers in vitro, and (2) insulin potentiates histamine release in the whole animal, induced by dextran or Con A, but it does not enhance release from isolated mast cells [4]. In the present study, PS did not enhance histamine release in vivo though it was effective in the presence of isolated mast cells.…”

Enhancement of the Anaphylactoid Reaction in Rats by Phenylalanine Enamine

“…The enhancing effect of PAE on the anaphylactoid reaction in rats resembles, in many ways, those of in sulin [1] and salicylate [3]. In all cases, the reaction is more intense at all stages.…”

Enhancement of the Anaphylactoid Reaction in Rats by Salicylates

“…The enhancing effect of sodium or copper salicy late on the anaphylactoid reactions in R rats resem bles, in many ways, that of insulin [1]. In both cases, there is a shorter onset of action, a higher peak of reaction, and a longer duration of effect.…”