Histamine release from isolated and intact mast cells of two types of genetically different rat

Abstract

A comparison has been made of the release of histamine from tissues of conventional rats, induced by injections of antigen, concanavalin A and clinical dextran, with that from rat isolated peritoneal mast cells. Concanavalin A was less active than dextran when injected into the skin or paws, but the reverse was found when the substances were tested on isolated cells. Similar results were obtained when a pure line of rats relatively resistant to dextran was used, concanavalin A being much more active on isolated cells than on intact mast cells. Antigen was equally active in the two types of rat. It is important, therefore, to state the experimental conditions when comparisons of the activities of histamine releasers are being made.

Forty Years of Pharmacological Research

“…More work is clearly needed to define its function but this polypeptide may in some way modify the dextran receptor in NR rats so that histamine release cannot be induced when dextran is injected. Similar results concerning the release of histamine have been found using isolated peritoneal mast cells from the two types of genetically different rats (West 1982a). …”

Antagonistic Analogs of Luteinizing Hormone-Releasing Hormone Are Mast Cell Secretagogues

“…However, since the reaction followed the first expo sure to the antagonist, it appeared that mast cell de granulation was not caused by an IgE-dependent mechanism but was due to direct interaction of antag onist and the mast cell membrane [6]. Thus, we hypothesized that the edematous reactions seen in rats following injection of LHRH antagonists were anaphylactoid in nature.…”

Histamine Releasers and Rat Mast Cells

“…Histamine release from isolated mast cells may not always accurately represent events taking place with in the body, and large differences have already been reported in the relative activities of some histamine releasing agents when tested under in vitro conditions and in the whole animal [3]. For example, concana valin A was about 100 times more active than clinical dextran when tested on isolated cells, yet it was about 20 times less active when injected into rats.…”