Insignificant antimicrobial activities and phytochemical screening in different extracts of indian Ginseng

Abstract

Aim:The aim of present study is to investigate the antimicrobial activity of (Flower, Ripen fruit and calyx) of Withania somnifera (RUBL20668) extracts, in order to use them as a possible source of new antimicrobial substances against important human pathogens. Materials and Methods: Crude extracts of different parts of both species of W. somnifera were evaluated against some important bacteria (two Gram +ve and four Gram-ve bacteria). Staphylococcus aureus (Gram +ve), Bacillus Subtilis (Gram +ve), Escherichia coli (Gram-ve), Raoultella planticola (Gram-ve), Pseudomonas aeruginosa (Gram-ve), Enterobactor aerogens (Gram-ve), one yeast Candida albicans and one fungus Aspergillus flavus. The dried and powdered seeds were successively extracted with hexane, toluene, iso propyl alcohol, acetone and ethanol, using the soxhlet assembly. The antimicrobial activity assay was done by both disc diffusion and serial dilution methods. Result: The results indicate that all the extracts in different polar solvents did not show any antibacterial activity against S. aureus, E. coli, R. planticola or E. aerogens or any antifungal activity against A. flavus. Conclusion: All extracts in the different polar solvents did not have or had very less antibacterial and antifungal activities.

A Comprehensive Scientific Claims on Ethnobotany, Phytochemical Pharmacological and Toxicology of Withania somnifera (L.) Dunal

“… W. somnifera has been demonstrated to have potent antiinflammatory effects in a range of medical disorders. In cases of IBD brought on by TNBS, its root extract is anti-inflammatory and restores mucous membranes resolving necrosis, edoema, and neutrophil infiltration, as well as reversing the progression of the disease 69 . In a mouse model of lupus, researchers found that a powder prepared from the roots of this plant significantly reduced inflammation as measured by proteinuria, nephritis, interleukin (IL)-6, tumour Nitric oxide (NO), reactive oxygen species (ROS), and necrosis factor-alpha 70 .…”

Estimation of Bio-Active Compounds in Indian Deserted Plant Euphorbia Caducifolia (Danda Thor) by Gas Chromatography-Mass Spectrometry

“…The collected plant materials were shade dried till constant weight was achieved 9 . The plant material were powered with the help of grinder 10 and passed through 40mm meshes and stored in clean container for further use 11 . The dried powder material was extracted with methanol by using the Soxhlet apparatus 12 for 18 hours at a temperature not exceeding the boiling point of the respective solvent 13,14 .…”

Estimation of Bio-active Compounds in Stem of Anogeissus rotundifolia (Indrdok) by Gas Chromatography-Mass Spectrometry.

“…The collected plant materials were shade dried, powered with the help of grinder (Singariya et al, 2012m) and passed through 40mm meshes and stored in clean container for further use (Singariya et al, 2012o). The dried powder material was extracted with acetone by using the Soxhlet apparatus (Subramanian and Nagarajan, 1969) for 18 hours at a temperature not exceeding the boiling point of the respective solvent (Singariya et al, 2013b; Singariya et al, 2012k).…”