Estimation of Bio-active Compounds in Stem of Anogeissus rotundifolia (Indrdok) by Gas Chromatography-Mass Spectrometry.

Abstract

The investigation was carried out to determine the possible bioactive components of methanolic extracts of Anogeissus rotundifolia (stem) using Gas chromatography-Mass spectrometry (GC-MS). All the samples were dried firstly at 60°C for 2 days in an oven after that leave it on room temperature. They were then macerated to powder form with a mixer grinder. The powder was stored in air sealed polythene bags at room temperature before extraction. The chemical compositions of the methanolic extracts of Anogeissus rotundifolia (stem) were investigated using Thermo G C 1300 and "TSQ 8000 "Triple quadruple GC-MSMS SYSTEM with auto sampler Al 1310 Gas chromatography-Mass spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis of the extract reveals the identification of forty nine compounds. This is the first report of identification of components from the stem of Anogeissus rotundifolia by GC-MS. Most of the compounds in the list are bioactive and possess medicinal properties.