Insights into geohelminth contamination in Bangladesh: feasibility of a modified diagnostic method and prevalence study

Abstract

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Molecular evidence of hookworms in public environment of Bangladesh

“…Samples were collected from different local spots within Sylhet metropolitan area (Lakkatura, Tilagor, Bondor, and Varthakhola), Bangladesh which have been recognized as soil-transmitted helminths endemic areas 8 . Using a cross-sectional study design and purposive sampling approach, 80 soils and 50 canine fecal samples that had been defecated in public areas were collected between January and June 2021.…”

“…Parasitological assessment of soil samples was processed using the modified flotation method (Sheather's sugar flotation), whereas the fecal samples were treated using the formalin-ether sedimentation technique. The specific gravity of the flotation solution was adjusted to 1.27 and a small quantity of 10% formalin was added to the prepared flotation solution to facilitate transitory preservation 8 . Observed larvae from soil and fecal samples were collected using pipette under stereomicroscope (Olympus ACH-1X, Japan) and preserved in fixatives (10% formalin and 95% ethanol).…”

An update of intestinal helminth infections among urban slum communities in Bangladesh

“…This may be due to geographical differences and the socio-economic and sanitary status of the study population. It is also likely that the environment is highly contaminated; a high concentration of geohelminths was reported in the study setting ( Nath et al., 2021 ). Among the positive individuals, 65.8% were school-aged children, although they had been subjected to one or more rounds of anthelmintic drug administration in the frame of national mass drug administration (MDA) campaigns.…”

“…The specimens were checked for identification number and divided into two aliquots. Parasitological assessment was performed using a modified formalin ether sedimentation method ( Nath et al., 2021 ). In brief, one aliquot was used for the formol-ether concentration technique, and the other aliquot was kept for cross-checking.…”

Insights to helminth infections in food and companion animals in Bangladesh: Occurrence and risk profiling

“…One aliquot was used for the formol-ether concentration technique, while the other was kept for cross-checking. Parasitological assessment was done by modified formalin ether sedimentation method ( Nath et al, 2021a ) and sugar floatation techniques ( Nath et al, 2021b ). For modified formalin ether sedimentation technique, the procedure began with thorough mixing of fecal suspension and sieving through gauze into a test tube.…”