Developing guidelines for designing robot-inclusive spaces has been challenging and resource-intensive, primarily relying on physical experiments and observations of robot interactions within the built environment. These conventional methods are often costly, time-consuming, and labour-intensive, demanding manual intervention. To address these limitations, this study explores the potential of using digital twins as a promising solution to offer detailed insights, reducing the dependence on physical experiments for studying robot-built environment interactions.Although the concept of digital twins is popular in many domains, the use of digital twins for this specific problem has not been explored yet. A novel methodology for assessing existing built environment guidelines by incorporating them as an architectural digital twin asset within robot simulation software is proposed in this regard. By analysing the digital interactions between robots and the architectural digital twin assets in simulations, the compatibility of the environment with robots is evaluated, ultimately contributing to enhancing these guidelines to be robot-inclusive. The ultimate goal is to create environments that are not only inclusive but also readily accessible to Autonomous Mobile Robots (AMRs). With this objective, the proposed methodology is tested on robots of different specifications to understand the robots’ interactions with different architectural digital twin environments and obstacles. The digital twin effectively demonstrates the capability of the proposed approach in assessing the robots’ suitability for deployment in the simulated environments. The gained insights contribute to improved comprehension and strengthen the existing design guidelines.