INRA-ResDur: the French grapevine breeding programme for durable resistance to downy and powdery mildew

Schneider, Christophe; Onimus, Christine; Prado, Emilce; Dumas, Vincent; Wiedemann‐Merdinoglu, Sabine; Dorne, M.A.; Lacombe, M.C.; Piron, Marie-Christine; Umar-Faruk, A.; Duchêne, Éric; Mestre, Pere; Merdinoglu, Didier

doi:10.17660/actahortic.2019.1248.30

Cited by 25 publications

(30 citation statements)

References 8 publications

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“…7 ). Therefore, a combination of several resistance loci is of great importance for grapevine breeding [ 3 , 6 , 34 , 36 ]. Despite this, our results demonstrate that P. viticola isolates overcoming two resistance loci can evolve.…”

Section: Discussionmentioning

confidence: 99%

“…The durability of resistance of these new grapevine cultivars is of particular importance in viticulture, since grapevines are permanent fruit crops growing for up to 30 years in vineyards [ 35 ]. In the recent years the first FRC with polygenic resistance against P. viticola have been registered [ 36 – 38 ]. However, detailed knowledge of the mechanisms underlying the resistance conferred by the loci used in these new breeding lines is lacking.…”

Section: Introductionmentioning

confidence: 99%

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Grapevine Rpv3-, Rpv10- and Rpv12-mediated defense responses against Plasmopara viticola and the impact of their deployment on fungicide use in viticulture

et al. 2021

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Background The high susceptibility of European grapevine cultivars (Vitis vinifera) to downy mildew (Plasmopara viticola) leads to the intensive use of fungicides in viticulture. To reduce this input, breeding programs have introgressed resistance loci from wild Vitis species into V. vinifera, resulting in new fungus-resistant grapevine cultivars (FRC). However, little is known about how these different resistance loci confer resistance and what the potential reduction in fungicide applications are likely to be if these FRCs are deployed. To ensure a durable and sustainable resistance management and breeding, detailed knowledge about the different defense mechanisms mediated by the respective Rpv (Resistance to P. viticola) resistance loci is essential. Results A comparison of the resistance mechanisms mediated by the Rpv3–1, Rpv10 and/or Rpv12-loci revealed an early onset of programmed cell death (PCD) at 8 hours post infection (hpi) in Rpv12-cultivars and 12 hpi in Rpv10-cultivars, whereas cell death was delayed in Rpv3-cultivars and was not observed until 28 hpi. These temporal differences correlated with an increase in the trans-resveratrol level and the formation of hydrogen peroxide shortly before onset of PCD. The differences in timing of onset of Rpv-loci specific defense reactions following downy mildew infection could be responsible for the observed differences in hyphal growth, sporulation and cultivar-specific susceptibility to this pathogen in the vineyard. Hereby, Rpv3- and Rpv12/Rpv3-cultivars showed a potential for a significant reduction of fungicide applications, depending on the annual P. viticola infection pressure and the Rpv-loci. Furthermore, we report on the discovery of a new P. viticola isolate that is able to overcome both Rpv3- and Rpv12-mediated resistance. Conclusion This study reveals that differences in the timing of the defense reaction mediated by the Rpv3-, Rpv10- and Rpv12-loci, result in different degrees of natural resistance to downy mildew in field. Vineyard trials demonstrate that Rpv12/Rpv3- and Rpv3-cultivars are a powerful tool to reduce the dependence of grape production on fungicide applications. Furthermore, this study indicates the importance of sustainable breeding and plant protection strategies based on resistant grapevine cultivars to reduce the risk of new P. viticola isolates that are able to overcome the respective resistance mechanism.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Grapevine Rpv3-, Rpv10- and Rpv12-mediated defense responses against Plasmopara viticola and the impact of their deployment on fungicide use in viticulture

et al. 2021

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“…One approach is a combination of classical selection with marker-assisted selection (MAS) for introducing certain loci into new varieties [88]. Different countries have developed various new breeding programs aimed at obtaining grape varieties combining disease resistance with high quality berries [4,[89][90][91][92]. The use of molecular markers allows to speed up and to make the selection process cheaper, and also to ensure the creation of varieties carrying new combinations of genes, which are almost impossible by traditional breeding methods [93].…”

Section: Figurementioning

confidence: 99%

Grapevine Gene Systems for Resistance to Gray Mold Botrytis cinerea and Powdery Mildew Erysiphe necator

et al. 2022

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Grapevine is one of the world’s most economically important fruit crops. It is known that Vitis vinifera is a host for a large number of pathogenic agents, which significantly reduce the yield and berry quality. This forces the agronomists to use a huge amount of fungicides. Over the last few decades, alternative methods for solving this problem have been developed and continue to be developed. Such new technologies as marker-assisted selection, bioengineering of the rhizosphere, genetic engineering (transgenesis, cisgenesis and intragenesis) allow the production of pathogen-resistant cultivars. However, they are linked to a number of problems. One of the most promising methods is the creation of modified non-transgenic cultivars via CRISPR/Cas9-targeted mutagenesis. Therefore, researchers are actively looking for target genes associated with pathogen resistance and susceptibility. This review elucidates the main mechanisms of plant—pathogen interactions, the immune systems developed by plants, as well as the identified genes for resistance and susceptibility to the biotrophic pathogen Erysiphe necator and the necrotrophic pathogen Botrytis cinerea.

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“…Four new resistant varieties: 'Artaban', 'Floreal', 'Vidoc' and 'Voltis' were registered in 2018, and a range of about 20 cultivars should be registered additionally by 2024. This project is the result of collaboration between INRA and IFV, French Institute of Vine and Wine and other European Institutes of breeding [5].…”

Section: Introductionmentioning

confidence: 99%

Immunogenic breeding program. Stage I-phytopathological screening of the grape gene pool

et al. 2021

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At the present stage of science development it is necessary to develop and implement the grape breeding programs for immunity basing on the international level of knowledge about the genetics of immunity to pathogens that cause culture diseases. The extensive material on genes of grape resistance to Plasmopara viticola and Erysiphe necator (Uncinula necator), which cause mildew and oidium diseases, has been collected thanks to the MAS technology. There is an evidence of necessity to pyramidize genes of resistance to these pathogens in one progeny genome for consistent field resistance to the complex of these pathogens. However, even at present, the issue of study the transfer of specific genes of resistance from parents to the progeny, their combining ability in one genome, and gene expression during pathogenesis remains relevant. This publication discusses the formation of grape breeding program in the FSBSI Institute Magarach of the RAS on introgression of resistance genes of the species Vitis rotundifolia (Muscadinia rotundifolia), and also presents the results of phytopathological screening of populations resulting from crossing with this species.

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INRA-ResDur: the French grapevine breeding programme for durable resistance to downy and powdery mildew

Cited by 25 publications

References 8 publications

Grapevine Rpv3-, Rpv10- and Rpv12-mediated defense responses against Plasmopara viticola and the impact of their deployment on fungicide use in viticulture

Grapevine Rpv3-, Rpv10- and Rpv12-mediated defense responses against Plasmopara viticola and the impact of their deployment on fungicide use in viticulture

Grapevine Gene Systems for Resistance to Gray Mold Botrytis cinerea and Powdery Mildew Erysiphe necator

Immunogenic breeding program. Stage I-phytopathological screening of the grape gene pool

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