Inorganic nutrient manipulation for highly improved in vitro plant regeneration in finger millet—Eleusine coracana (L.) Gaertn.

Kothari, S. L.; Avasthi, Kalpana; Kumar, Satish

doi:10.1079/ivp2004564

Cited by 61 publications

(29 citation statements)

References 26 publications

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“…However, in most of the earlier studies, the basic nutrient medium was supplemented with a variety of adjuvant such as coconut water (Mathias and Simpson 1986), amino acid (glutamine) and organic nitrogen source (casein hydrolysate, CH) (Mendoza and Kaeppler 2002), glutamine, CH and proline (Ganeshan et al 2006), CH and proline (Chauhan et al 2007), glutamine, asparagine and CH (Bi and Wang 2008) and aspartic acid, glutamine, proline, tryptophan and CH (Yu et al 2008). Modification of ammonium nitrate concentration in media has been shown to promote callus induction (Constabel and Shyluk 1994), somatic embryogenesis in cereals (Grimes and Hodges 1990;Kothari et al 2004;Greer et al 2009) and genetic transformation (Greer et al 2009;Boyko et al 2009). The beneficial effect of NH 4 NO 3 on callus induction and regeneration has been probably by altering the sensitivity of the explants to auxin levels (Greer et al 2009).…”

Section: Resultsmentioning

confidence: 99%

Plant regeneration from mature embryo of commercial Indian bread wheat (Triticum aestivum L.) cultivars

Parmar

Sainger

Chaudhary

et al. 2012

Physiol Mol Biol Plants

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Section: Resultsmentioning

confidence: 99%

Plant regeneration from mature embryo of commercial Indian bread wheat (Triticum aestivum L.) cultivars

Parmar

Sainger

Chaudhary

et al. 2012

Physiol Mol Biol Plants

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“…Por otra parte, existen reportes de micropropagación de algunas variedades de castaño, las cuales son específicas y esto se debe a que en cultivo de tejidos el crecimiento y el desarrollo de los explantos son influenciados en gran medida por el genotipo y por los reguladores de crecimiento suministrados al medio de cultivo (Kothari et al 2004).…”

Section: Resultados Y Discusiónunclassified

Efecto del genotipo y fuente de citoquinina en la etapa de iniciación de cultivo in vitro de tejido adulto de Castanea sativa Mill.

Larson¹,

Hasbún²,

Jofré³

et al. 2017

Gayana Bot.

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“…The phenomenon of multiple copy integration is commonly encountered in particle-bombardment-mediated genetic transformation of plants [15][16][17][18]. In earlier studies, a variable number of incorporated transgene copies ranging between 1 and 20 were reported in diverse crop plants [19][20][21][22][23][24][25][26][27][28][29][30].…”

Section: Figure: 7 Extraction Of Total Rna and Rt-pcr Analysismentioning

confidence: 99%

In vitro Regeneration and Optimization of Conditions for transformation methods in Pearl millet, Pennisetum glaucum (L.)

Naravula¹,

Maheshwari²,

Naidu³

et al. 2016

IJCBS

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Pearl millet is a dual-purpose crop used for grain and fodder and is grown primarily in Asia and Africa, where it occupies some 27 million ha. It is capable of growing on some of the poorest soils in dry, hot regions of Africa and Asia, where, as a poor man's source of dietary energy, it sustains a large proportion of the populace. It is also grown in other countries where, under relatively more favorable conditions, it provides grain for bullocks, dairy cows, and poultry. Downy mildew caused by Sclerospora graminicola (Sacc.) J. Schroet. is the most widespread and destructive disease of pearl millet causing severe economic losses. New genes can be introduced into this plant through Agrobacterium mediated and bombardment genetic transformation for its genetic improvement, which is dependent on the availability of suitable in vitro techniques. An efficient regeneration system has been developed for in vitro culture of pearl millet (Pennisetum glaucum L.) from the immature inflorescence. High frequency callus and shoot regeneration was obtained on Murashige and Skoog nutrient agar medium supplemented with 2mg/l 2,4-D and 0.2 mg/l NAA, 2 mg/l Kinetin and 30 g/l sucrose. On transfer to soil, the regenerated plantlets survived and appeared to be morphologically similar to the normal seed-grown plants. Histological analysis revealed the de novo origin of shoots from embryogenic callus in in vitro cultured pearl millet. Parameters affecting transformation were optimized by assaying phosphinothricin resistance to transformed calli and basta test for these leaves of plantlets after transferring to pots. These tissues appear to be susceptible to Agrobacterium infection and Particle gun flow mediated transformation carrying pCAMBIA2300 with osmotin and chitinase double construct and pPUR with bar genes, as well as shoot multiplication. The embryogenic callus was found competent to take up the DNA, which was monitored by transient bar gene with GV2600 at 0.6 O.D for Agrobacterium infection and 1µg/µl plasmid DNA from E.coli for cobombardment was found to be compatible in giving transgenics.

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Inorganic nutrient manipulation for highly improved in vitro plant regeneration in finger millet—Eleusine coracana (L.) Gaertn.

Cited by 61 publications

References 26 publications

Plant regeneration from mature embryo of commercial Indian bread wheat (Triticum aestivum L.) cultivars

Plant regeneration from mature embryo of commercial Indian bread wheat (Triticum aestivum L.) cultivars

Efecto del genotipo y fuente de citoquinina en la etapa de iniciación de cultivo in vitro de tejido adulto de Castanea sativa Mill.

In vitro Regeneration and Optimization of Conditions for transformation methods in Pearl millet, Pennisetum glaucum (L.)

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