Efecto del genotipo y fuente de citoquinina en la etapa de iniciación de cultivo in vitro de tejido adulto de Castanea sativa Mill.

Larson, A Carmen Gloria; Hasbún, Z Rodrigo; Jofré, V María Paz; Sánchez‐Olate, Manuel; Rios, Liliana

doi:10.4067/s0717-66432017005000319

Cited by 3 publications

(6 citation statements)

References 34 publications

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“…Furthermore, most of the times fertilization recommendations for chestnut trees are highly empirical, mainly based on personal experience of the growers and researchers, without taking into account scientific data (soil fertility and determination of leaf nutrient concentrations) (Chatzistathis, unpublished data). Most of the existing published papers on C. sativa Mill are mainly focused on the improvement of its germination and micro-propagation [4][5][6], as well as on the non-destructive leaf area estimation [7]. In addition, Silvanini et al [8] investigated the altitude effects on fruit morphology of two chestnut cultivars.…”

Section: Introductionmentioning

confidence: 99%

Zeolite and Vermiculite as Inorganic Soil Amendments Modify Shoot-Root Allocation, Mineral Nutrition, Photosystem II Activity and Gas Exchange Parameters of Chestnut (Castanea sativa Mill) Plants

Chatzistathis¹,

Papaioannou

Γιαννακούλα

et al. 2021

Agronomy

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One of the most challenging topics for the sustainable agriculture is how to decrease high fertilization rates. A pot experiment, exploring the effects of zeolite (ZEO) and/or vermiculite (VER) as soil amendments, comparing to the soil application of a controlled release fertilizer (CRF), was realized in chestnut plants. Various parameters related to soil fertility, and plant growth, nutrition, and physiology were investigated to gain knowledge towards more sustainable management. After ZEO application and in comparison to CRF, an impressive boost in soil K was achieved. Moreover, soil P and Zn levels were higher in the VER-treated soil, compared to CRF. Leaf K and Ca concentrations were significantly higher in ZEO, compared to the VER treatment; the highest foliar N and Zn concentrations were measured in CRF and VER, respectively. However, significantly lower foliar Mn and Cu were found in VER. The highest root biomass produced in the ZEO treated plants. For most nutrients, their total uptake per plant was higher in CRF and ZEO. Finally, photosynthetic rates were higher in VER (mainly due to non-stomatal factors) and CRF (mainly due to stomatal factors). Our data open a discussion towards the application of ZEO and/or VER as soil amendments in chestnut nurseries and orchards, aiming at partially decreasing fertilization rates and boosting sustainable nutrient management.

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Section: Introductionmentioning

confidence: 99%

Zeolite and Vermiculite as Inorganic Soil Amendments Modify Shoot-Root Allocation, Mineral Nutrition, Photosystem II Activity and Gas Exchange Parameters of Chestnut (Castanea sativa Mill) Plants

Chatzistathis¹,

Papaioannou

Γιαννακούλα

et al. 2021

Agronomy

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“…The results highlight the significantly positive influence of the treatments in shoot growth and multiplication. Overall, this difference in responses is since BA significantly stimulates the formation of axillary buds, while the TDZ response is associated with the formation of adventitious buds [49].…”

Section: Experimental Phase 1: In Vitro Multiplicationmentioning

confidence: 99%

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

Luz,

Román,

Mar

et al. 2023

Preprint

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Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the multiplication of seedlings, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL-1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds, shoots, and seedling height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L-1 of BA and 0.1 mg L-1 of TDZ, as well as the MS base culture medium. With 0.1 mg L-1 of BA, 52-mm height, 1.36 shoots, and 9.22 buds were obtained, surpassing the MS control (MS culture medium alone); likewise, twice more roots (80%) and a 14% reduction in the bud structure were packed in 50 repetitions. The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L-1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. The results demonstrated that the induction of callogenesis in S. compositum has a better start from the stem in a medium with 2.0 mg L-1 of 2,4-D, whose value was 4.89 —equivalent to 97.8% callus formation around the explant. The addition of 500 mg L-1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callogenesis of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.

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“…The results highlight the positive influence of the treatments on shoot growth and multiplication. Overall, this difference in responses occurs because BA stimulates the formation of axillary buds, while the TDZ response is associated with the formation of adventitious buds [51].…”

Section: Experimental Phase 1: In Vitro Multiplicationmentioning

confidence: 99%

“…In the case of BA, treatments 0.6 mg L −1 , 0.8 mg L −1 , and 1.2 mg L −1 formed more calli than treatments that had the lowest concentrations (0.1, 0.2, 0.4, and 1.0 mg L −1 ). For example, in Castanea sativa, Mill Larson et al [51] demonstrated that two of their TDZ and BA treatments recorded a higher (80%) formation of basal callus, which improved the establishment of the shoot. They also mention that the basal callus accumulates substances and hormones necessary for the in vitro response, which, according to Meier-Dinkel et al [58], suggests a relationship between callus formation and the establishment of the shoot.…”

Section: Experimental Phase 1: In Vitro Multiplicationmentioning

confidence: 99%

“…With the addition of BA to the medium, the "rosette" structure of packed buds (Figure 3) decreases. The formation of these structures may be caused by a loss of morphogenic competence in the mature material (morphological, biochemical, and molecular differences); consequently, the ontogenic age of the plant material causes the absence of elongation, as younger plant explants lead to a greater elongation rate of the axillary buds [51]. Therefore, it is suggested that BA acted as a rejuvenating agent for the material.…”

Section: Experimental Phase 1: In Vitro Multiplicationmentioning

confidence: 99%

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In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product

de la Luz,

Román,

Jorge

et al. 2024

Horticulturae

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Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the shoot propagation, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL−1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds and shoots and the shoot height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L−1 of BA and 0.1 mg L−1 of TDZ, as well as the MS base culture medium. The validation of the results of the first phase (0.1 mg L−1 of BA) was compared with the MS in an independent experiment against the control (n = 50 repetitions), obtaining a height of 52 mm, 1.36 shoots, and 9.22 buds, suggesting that this concentration is adequate for the purpose, surpassing the MS control (MS culture medium alone). Of the total volume of roots obtained with packed bud structure in the previous experimental sample, it was reduced to 14% (n = 50). The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L−1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. Callus induction in S. compositum was better when using the stem in a medium with 2.0 mg L−1 of 2,4-D with a value of 97.8% around the explant. The addition of 500 mg L−1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callus formation of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.

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Efecto del genotipo y fuente de citoquinina en la etapa de iniciación de cultivo in vitro de tejido adulto de Castanea sativa Mill.

Cited by 3 publications

References 34 publications

Zeolite and Vermiculite as Inorganic Soil Amendments Modify Shoot-Root Allocation, Mineral Nutrition, Photosystem II Activity and Gas Exchange Parameters of Chestnut (Castanea sativa Mill) Plants

Zeolite and Vermiculite as Inorganic Soil Amendments Modify Shoot-Root Allocation, Mineral Nutrition, Photosystem II Activity and Gas Exchange Parameters of Chestnut (Castanea sativa Mill) Plants

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product

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