Inoculation of corn silage with Lactobacillus plantarum and Bacillus subtilis associated with amylolytic enzyme supply at feeding. 2. Growth performance and carcass and meat traits of lambs

Abstract

The objective of this research was to investigate the effects of inoculation of corn silage and dietary supplementation of amylolytic enzymes, and their interaction, on lamb growth performance and carcass and meat traits. Whole-crop corn forage was treated either with water (untreated) or with Lactobacillus plantarum MA 18/5U at 1 × 10 5 cfu/g fresh forage combined with Bacillus subtilis AT553098 at 1 × 10 5 cfu/g fresh forage (inoculated) and ensiled for 170 d. Forty non-castrated Texel × Dorper male lambs were arranged in a randomized block design (n = 10) and then assigned to one of four diets, as follows: 1) untreated corn silage with no amylolytic enzyme supply (NI-NA); 2) untreated corn silage with amylolytic enzyme supply (NI-WA); 3) inoculated corn silage with no amylolytic enzyme supply (WI-NA); and 4) inoculated corn silage with amylolytic enzyme supply (WI-WA). α-Amylase (602 dextrinizing units/kg of dry matter (DM)) was supplied to the lambs at total mixed ration delivery. Inoculation of corn silage increased (P = 0.003) both digestible energy (DE) and metabolizable energy (ME) intake by 1.4 and 1.2 MJ/kg DM, respectively, and also resulted in higher average daily gain (ADG; P = 0.023) of lambs (233 vs. 212 g/d in lambs fed NI diet). In contrast, dietary supplementation with amylolytic enzymes had no effect on the DM intake (P = 0.90) or ADG (P = 0.15) of lambs. Moreover, inoculation, enzyme supplementation, and their interaction had no significant effects on carcass traits (P ≥ 0.06), commercial cut yield (P ≥ 0.26), chemical composition of meat (P ≥ 0.13), and meat tenderness and color (P ≥ 0.11). In terms of the fatty acid composition of longissimus muscle, inoculation of corn silage increased concentrations of saturated fatty acids (SFA; P = 0.035) from 46.2% to 47.6%, and reduced concentrations of unsaturated fatty acids (UFA; P = 0.035) from 53.8% to 52.5%, compared to the NI diet. Inoculation also reduced the UFA:SFA ratio (P = 0.031) from 1.17 to 1.11, a small but significant difference. Dietary supplementation with amylolytic enzymes did not alter (P ≥ 0.09) SFA, UFA, and polyunsaturated

Effect of Amylolytic and Cellulolytic Enzymes on Whole Plant Corn Silage: Characteristics of Silage and Animal Digestion

“…Despite of amylolytic enzymes have potential to increase nutrients digestibility by acting on starch-protein matrix, which could 230 enhance microbial attachment and enzymatic digestion of starch granules (Giuberti et al, 2014), no beneficial responses were observed 231 on nutrient intake and digestibility. The same was observed by Lara et al (2018), evaluating lambs fed corn silage with inoculant alone 232 or in combination with amylolytic enzymes. 233 Enzyme aditives are mostly applied in combination with bacterial inoculants (Muck et al, 2018), as observed in this study, where 234 we added to all treatments microbial aditive composed by Lactobacillus plantarum and Pediococcus acidilactici.…”

Effects of feeding corn containing an alpha-amylase gene on the performance and digestibility of growing cattle

“…DMI tended to be greater ( P < 0.08) for calves fed EFC-S over the entire 91-d trial, and this tendency became apparent as early as day 42 of the trial. Lara et al (2018b) researched effects of feeding corn silage diets with or without an amylolytic enzyme supplemented to lambs. They found that providing 602 dextrinizing units of alpha-amylase/kg DM in the total mixed ration had no effect on DMI or ADG, and, although not significant, G:F was improved by 4.8% for lambs fed corn silage with an alpha-amylase supplement.…”

Effects of different dietary energy levels on growth performance, meat quality and nutritional composition, rumen fermentation parameters, and rumen microbiota of fattening Angus steers

“…The concentration of NH 3 -N is dependent on dietary crude protein levels and the rate of microbial breakdown and metabolism. Higher concentrations of NH 3 -N generally favor microbial protein synthesis ( Basso et al, 2018 ; Lara et al, 2018 ). In this study, no significant differences in rumen fluid NH 3 -N concentrations were observed among the groups.…”