Innovative ultra-sensitive spectrofluorimetric method for nanogram detection of doripenem monohydrate in human plasma, urine and pharmaceutical formulation

Salman, Baher I.; Hussein, S. A.; Ali, Marwa F.; Marzouq, Mostafa A.

doi:10.1016/j.microc.2018.12.018

Cited by 16 publications

(29 citation statements)

References 17 publications

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“…The coloured product formed from the reaction between SPEC and NBD-Cl was pH dependent and was produced using 0.1 M borate buffer (pH 9.2 ± 0.2) with a volume of 1.5 ± 0.25 ml giving the most suitable buffer conditions (Figure 3a and Supporting Information Figure S1). [23,26] Expanding the pH range of the 0.1 M borate buffer to values higher than 9.5 caused a decrease in relative fluorescence intensity (RFI) due to increase in hydroxide group concentration that prevented the reaction between SPEC and NBD-Cl; this result concurred with those of previously published studies. [12,23,26] FIGURE 2 (a) Absorption and (b) excitation (460 nm) and emission (530 nm) spectra of the reaction product formed between NBD-Cl and SPEC (2 μg ml −1 and 150 ng ml −1 respectively)…”

Section: Effect Of Phsupporting

confidence: 89%

“…Stoichiometry between SPEC and NBD-Cl was checked using a continuous variation method (Job's method), [22] as described previously [20,23,24] by preparing 1 × 10 −4 M SPEC and NBD-Cl.…”

Section: Stoichiometry Mechanismmentioning

confidence: 99%

“…[26] The addition of 1.0 ± 0.1 ml of 2 M HCl was required to quench the NBD-OH fluorescence, as shown in Figure S2, without changing the fluorescence of the yielded product. [12,23,26]…”

Section: Effect Of Volume Of Hclmentioning

confidence: 99%

“…The optimum solvent for the reaction was used following a trial of numerous solvents. Methanol was observed to be the optimum solvent, as it gave the highest fluorescence response with great reproducibility and low blank scanning [23] as represented in Figure S3.…”

Section: Effect Of Diluting Solventsmentioning

confidence: 99%

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Development of sensitive benzofurazan‐based spectrometric methods for analysis of spectinomycin in vials and human biological samples

et al. 2019

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Spectinomycin hydrochloride (SPEC) is an aminoglycoside antibiotic that has a broad spectrum against several bacterial strains of humans and veterinary infections.However, SPEC lacks a fluorophore or chromophore and this lack makes its analysis a challenge. Our study provides a simple, sensitive and low-cost spectrofluorimetric/spectrophotometric method based on the reaction between secondary amine groups and a benzofurazan reagent using borate buffer (pH 9.2). The yielding compound was measured fluorimetrically at 530 nm (excitation at 460 nm) with colorimetry at 410 nm. The calibration curves ranged from 30 to 400 ng ml −1 and from 0.2 to 6 μg ml −1 for spectrofluorimetric and spectrophotometric analyses, respectively. The limits of detection were calculated to be 4.15 ng ml −1 and 0.05 μg ml −1 for spectrofluorimetric and spectrophotometric processes, respectively.The ultra-affectability and high selectivity of the proposed method permitted analysis of SPEC in the dosage form and in human plasma samples, with good recoveries of about 101.19 and 97.11%, respectively, without any matrix interference. The proposed strategy was validated using International Conference on Harmonization standards and validated bioanalytically using USFDA recommendations.

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Section: Effect Of Phsupporting

confidence: 89%

“…Stoichiometry between SPEC and NBD-Cl was checked using a continuous variation method (Job's method), [22] as described previously [20,23,24] by preparing 1 × 10 −4 M SPEC and NBD-Cl.…”

Section: Stoichiometry Mechanismmentioning

confidence: 99%

“…[26] The addition of 1.0 ± 0.1 ml of 2 M HCl was required to quench the NBD-OH fluorescence, as shown in Figure S2, without changing the fluorescence of the yielded product. [12,23,26]…”

Section: Effect Of Volume Of Hclmentioning

confidence: 99%

Section: Effect Of Diluting Solventsmentioning

confidence: 99%

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Development of sensitive benzofurazan‐based spectrometric methods for analysis of spectinomycin in vials and human biological samples

et al. 2019

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“…From the literature survey, different analytical methods have been reported for the analysis of DOR such as spectrophotometry, 2-5 spectrouorimetry, 6 high-performance liquid chromatography (HPLC), [7][8][9] high-performance thin layer chromatography (HPTLC) 10 and electrochemical 11 methods. From this survey, only one spectrouorimetric method was reported for the determination of DOR.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive cadmium sulphide quantum dots as a fluorescent probe for estimation of doripenem in real human plasma: application to pharmacokinetic study

et al. 2020

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Zinc(II) complexation strategy for ultra‐sensitive fluorimetric estimation of molnupiravir: Applications and greenness evaluation

Salman

Hara

Deeb

et al. 2023

Archiv der Pharmazie

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The endemicity of the pandemic coronavirus disease 2019 (COVID-19) infection proved to be transitional only. Spikes are forming again in 2023, and high expectations are returning for reinfections and viral mutations. Molnupiravir (MOL) has been approved as an oral antiviral drug for the treatment of the COVID-19 causative virion. Therefore, the development of an ultrasensitive, instantaneous, and cost-effective method for the quantification of MOL in real plasma samples and formulated dosage form are mandatory. The proposed approach is based on the synthesis of a MOL metal-chelation product. MOL as a ligand was chelated with 1.0 mM zinc(II) in an acetate buffer (pH 5.3). After illumination at 340 nm, the intensity of the MOL fluorescence measured at 386 nm was increased by about 10-fold. The linearity range was found to be from 60.0 to 800.0 ng mL −1 with limit of quantitation (LOQ) of 28.6 ng mL −1 . Two methods were utilized for measuring the greenness of the proposed method (Green Analytical Procedure Index[GAPI] and analytical greenness metric [AGREE] methods), with results equal to 0.8.The binding stoichiometry of MOL with the zinc(II) ion was found to be 2:1. All the experimental parameters were optimized and validated using International Conference on Harmonization (ICH) and United States Food and Drug Administration (US-FDA) recommendations. Furthermore, the fluorescent probes were successfully utilized in real human plasma with high percentages of recovery (95.6%-97.1%) without any matrix interferences. The mechanism of fluorescent complex formation was confirmed using 1 H NMR in the presence and absence of Zn(II). The method was further utilized for testing content uniformity of MOL in its marketed capsule dosage forms.

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Innovative ultra-sensitive spectrofluorimetric method for nanogram detection of doripenem monohydrate in human plasma, urine and pharmaceutical formulation

Cited by 16 publications

References 17 publications

Development of sensitive benzofurazan‐based spectrometric methods for analysis of spectinomycin in vials and human biological samples

Development of sensitive benzofurazan‐based spectrometric methods for analysis of spectinomycin in vials and human biological samples

Highly sensitive cadmium sulphide quantum dots as a fluorescent probe for estimation of doripenem in real human plasma: application to pharmacokinetic study

Zinc(II) complexation strategy for ultra‐sensitive fluorimetric estimation of molnupiravir: Applications and greenness evaluation

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