Introduction. Universities start their education in their associated pre-universities using University principles of education and modern technologies. The study of the impact of specialized medical training on senior high school students, and the justification of approaches to the hygienic assessment of modern technologies for their specialized training is relevant. Material and methods. The study based on the pre-University of Sechenov University, attended by 181 students aged 15-17 years. The assessment of the psychophysiological status of students was performed using specialized questionnaires (Klimov, Kettel, Schulte, Munsterberg, Spielberg-Hannin, SAN). Physiological methods included the assessment of indices: CFFF, RBD, tremorometry. Results. Specialized medical training exceeds the weekly training load-38-40 hours. Individual project activities are allocated 20 hours per week. Simulation classes are held once a week for 90 minutes with a 5-minute break in the middle. The schedule of classes corresponds to the physiological performance curve. The duration of homework exceeds hygiene standards in 69.2% of students. Among the students, only one in four has a tendency to work in the “person - to-person” system. Mental performance, indices of CSCM, RDO of students in the dynamics of the school day are stable. Indices of tremorometry in simulation classes are stable and indicate a good state of coordination capabilities of the hands. In simulation activities, 53.5% of students have higher-than-average activity, which is 1.4 times more than in a traditional lesson. All students are characterized by a high level of anxiety in all types of educational activities. Conclusion. Training using design and research, digital, simulation, and phantom technologies is adequate to the physiological capabilities of the body of students and can be recommended for the use in medical profile training. Hygienic examination of technologies of profile training of pupils should be based on a comprehensive approach, including assessment of psychophysiological status of students, and also indices of a psychofunctional state students during the lessons.