Inmunohistochemical Expression of PCNA, p53 and bcl-2 in Pleomorphic Adenomas

Gordón-Núñez, Manuel Antônio; Godoy, Gustavo Pina; Soares, Rosilene Calazans; Souza, Lélia Batista De; Freitas, Roseana de Almeida; Queiroz, Lélia Maria Guedes

doi:10.4067/s0717-95022008000300010

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…As nuclear size is a very important parameter, which changes in dysplasia and reflects the intensified DNA synthesis, and the abnormalities of nuclear features have been reported in various types of carcinoma, 43 , 44 morphological characteristics of PCNA-IR nuclei were investigated. Obtained results revealed that there was no significant differences in diameter and area of nuclei after administration of PAMAM-NH 2 at concentrations up to 3 mg/mL ( Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of cationic polyamidoamine dendrimers’ dermal toxicity in the rat skin model

Winnicka¹,

Wróblewska²,

Sosnowska³

et al. 2015

DDDT

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Polyamidoamine (PAMAM) dendrimers are multi-branched, three-dimensional polymers with unique architecture, which makes these molecules attractive for medical and pharmaceutical applications. Using PAMAM as drug carriers for topical delivery might be beneficial as they only produce a transient effect without skin irritation. To evaluate the dermal toxicity of cationic PAMAM dendrimers generation 2 and generation 3, skin irritation studies were performed in vivo in the rat skin model. After 10 days topical application of various concentrations of PAMAM-NH2 (0.3 mg/mL, 3 mg/mL, 6 mg/mL, 30 mg/mL, 300 mg/mL), skin irritation was evaluated by visual, histopathological, and immunohistochemical examination. Microscopic assessment after hematoxylin-eosin staining revealed significant morphological changes of epidermal cells after application of PAMAM-NH2 at a concentration of ≥6 mg/mL. Morphological alterations of epidermal cells included cytoplasmic vacuolization of keratinocytes in the basal and spinous layers. Cytomorphological changes in keratinocytes, overall picture of the epidermis, and histopathological changes in the dermis were dose dependent. Detected alterations concerned hyperplasia of connective tissue fibers and leukocyte infiltration. Visible granulocyte infiltration in the upper dermis and sockets formed by necrotic, cornified cells in the hyperplastic foci of epithelium were also noted. Immunohistochemical analyses revealed that increased nuclear immunoreactivity to PCNA correlated with the concentration of PAMAM-NH2, but no significant differences in the cell proliferation activity in skin treated with PAMAM-NH2 generation 2 or generation 3 were observed. Significantly higher expression of PCNA extended throughout the skin layers might suggest abnormal cell proliferation, which, as a consequence, might even lead to neoplastic changes.

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Section: Resultsmentioning

confidence: 99%

Evaluation of cationic polyamidoamine dendrimers’ dermal toxicity in the rat skin model

Winnicka¹,

Wróblewska²,

Sosnowska³

et al. 2015

DDDT

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“…Cells with no P 53 expression can potentially replicate with damaged DNA, leading to malignant change (24). Many studies have correlated the expression of normal and mutant p53 with the differentiation, aggressiveness, and progression of salivary glands tumors, but the results are highly controversial (25–27). This study indicates that P 53 was negative for about 70% of PA cases.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical analysis of P₅₃and bcl-2 in benign and malignant salivary glands tumors

Al‐Rawi

Omer

Kawas

2010

Journal of Oral Pathology &Amp; Medicine

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Pleomorphic adenoma (PA) showed negative expression of P(53) and bcl-2 in 70% of cases, whereas all malignant salivary glands tumors were positive for P(53) and bcl-2. P(53) positive/bcl-2 positive immunostaining reaction was week in small size PA and was totally absent in larger lesions. However, all malignant tumors expressed P(53), with the highest record in low-grade adenocarcinoma (76%) and the lowest score was observed in both low-grade carcinoma in PA and adenocystic carcinoma. bcl-2 immunostaining was also assessed, the highest recorded score was in high-grade adeno cystic carcinoma (90%) and the lowest in both low-grade carcinoma ex-PA and low-grade cystic mucoepidermoid carcinoma (3%). P(53)/bcl-2 immunostaining reactivity could be helpful in demonstrating salivary glands tumor behavior in terms of progression and extent of invasion.

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“…In this study, 93.3% of PA, 96.6% of AdCC, and 100% of MEC samples showed PCNA expression, which was in line with the study of Gordón-Núñez et al . [ 29 ] and contrary to the studies of Russo et al . [ 30 ], Perez et al .…”

Section: Discussionmentioning

confidence: 63%

Immunohistochemical analysis of proliferating cell nuclear antigen and minichromosome maintenance complex component 7 in benign and malignant salivary gland tumors

et al. 2022

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Background: Proliferation markers have been used to determine the behavior and prognosis of benign and malignant tumors; this study was aimed to compare the immunohistochemical (IHC) expression of proliferating cell nuclear antigen (PCNA) and novel marker minichromosome maintenance complex component 7 (MCM7) in common salivary gland tumors including pleomorphic adenoma (PA), mucoepidermoid carcinoma (MEC), and adenoid cystic carcinoma (AdCC), to find a possible significant correlation between benign and malignant tumors. Materials and Methods: In this cross-sectional study, a total of 90 cases, including 30 PAs, 30 MECs, and 30 AdCCs, were collected. The IHC expressions of PCNA and MCM7 were evaluated. Their expressions were compared with each other and between benign and malignant tumors. Statistical analysis was performed by Chi-square and Tukey's test. P value was considered 0.05. Results: Out of 30 cases of PA, 28 cases (93.3%) were PCNA positive and 28 cases (93.3%) were MCM7 positive. In the AdCC cases, 29 cases (96.6%) were PCNA positive and 29 cases (96.6%) were MCM7 positive. In the MEC cases, all cases (100%) were PCNA positive and 23 cases (76.6%) were MCM7 positive. The labeling index (LI) of MCM7 and PCNA was evaluated, and this index was lower in MCM7 LI than PCNA in all tumors. The MCM7 and PCNA expression showed a significant difference in PA and MEC ( P < 0.001). Conclusion: PCNA expression was higher than MCM7 expression in salivary gland tumors. However, more studies are needed to evaluate the malignant activity of these tumors with group of markers such as MCM family members.

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Inmunohistochemical Expression of PCNA, p53 and bcl-2 in Pleomorphic Adenomas

Cited by 7 publications

References 14 publications

Evaluation of cationic polyamidoamine dendrimers’ dermal toxicity in the rat skin model

Evaluation of cationic polyamidoamine dendrimers’ dermal toxicity in the rat skin model

Immunohistochemical analysis of P₅₃and bcl-2 in benign and malignant salivary glands tumors

Immunohistochemical analysis of proliferating cell nuclear antigen and minichromosome maintenance complex component 7 in benign and malignant salivary gland tumors

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Inmunohistochemical Expression of PCNA, p53 and bcl-2 in Pleomorphic Adenomas

Cited by 7 publications

References 14 publications

Evaluation of cationic polyamidoamine dendrimers&rsquo; dermal toxicity in the rat skin model

Evaluation of cationic polyamidoamine dendrimers&rsquo; dermal toxicity in the rat skin model

Immunohistochemical analysis of P53and bcl-2 in benign and malignant salivary glands tumors

Immunohistochemical analysis of proliferating cell nuclear antigen and minichromosome maintenance complex component 7 in benign and malignant salivary gland tumors

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Evaluation of cationic polyamidoamine dendrimers’ dermal toxicity in the rat skin model

Evaluation of cationic polyamidoamine dendrimers’ dermal toxicity in the rat skin model

Immunohistochemical analysis of P₅₃and bcl-2 in benign and malignant salivary glands tumors