Injectable Reactive Oxygen Species‐Responsive Hydrogel Dressing with Sustained Nitric Oxide Release for Bacterial Ablation and Wound Healing

Abstract

Skin wound healing strategies that promote sustainable healing, prevent bacterial infections, and cause only mild irritation are urgently needed for open wound therapy. In this study, a system that consists of a reactive oxygen species (ROS)‐degradable hydrogel dressing, with encapsulated biosafe nitric oxide (NO) donor L‐arginine (L‐Arg), and a low level (100 × 10−6 m) of the household disinfectant hydrogen peroxide (H2O2) is established for sustainable wound therapy and disinfection. Under stimulation with a low level of H2O2, NO is sustainably generated from L‐Arg within the hydrogel dressing, which mediates macrophage and fibroblast chemotaxis to the wound site, as well as collagen synthesis, resulting in rapid wound healing and skin organ regeneration. The combination of H2O2 and NO also endows the system with a synergistic antibacterial ability to eliminate ampicillin‐resistant Escherichia coli. In addition, a full‐thickness skin wound model demonstrates that the ROS‐degradable hydrogel dressing, with a wound exudate adsorption ability, shows effective wound healing promotion and a good biocompatibility, without any hazardous residues and skin irritation. This ROS‐responsive, degradable, synergistic wound healing system provides an alternative strategy for sustainable wound treatment, both at home and in the clinic.

Light‐Programmable Nanocomposite Hydrogel for State‐Switchable Wound Healing Promotion and Bacterial Infection Elimination

“…Open wounds with skin disorders can persist for months, which can lead to life-threatening bacterial infections and wound DOI: 10.1002/adhm.202201565 damage. [1] Thus, it is important to develop wound dressings to combat pathogens as well as promote wound healing. [2] However, the antimicrobial and tissue regeneration properties of wound dressing materials are often contradictory.…”

Multifunctional antibiotics-free hydrogel dressings with self-regulated nitric oxide-releasing kinetics for improving open wound healing

“…With the increase of NO concentration released from GCNO hydrogels, the antibacterial effect became more obvious, which is consistent with the experimental results of Yu et al and also in agreement with the concentration-dependent broad-spectrum antibacterial activity of NO. 26 In addition, the hydrogel antibacterial zone diameter of E. coli is larger than that of S. aureus in each group (Fig. 4(E) and (F)), which proved that E. coli with thinner cell walls is more sensitive to NO-induced bacterial elimination.…”

Enhanced Bacterial‐Infected Wound Healing by Nitric Oxide‐Releasing Topological Supramolecular Nanocarriers with Self‐Optimized Cooperative Multi‐Point Anchoring

“…Therefore, the loss of NO due to insufficient contact with bacteria, which was a common problem of previous NO carriers, [32] can be avoided and the bioavailability of NO was improved by the designed topological structure, leading to the massive mortality of MRSA. [33] Bacterial biofilm is the key barrier of bacteria to resist external stimuli and improve self-defense. [34] Biofilm infections bring challenges to the clinic, including recurrent infections, chronic diseases, antibiotic resistance, etc.…”