Injectable hydrogel for co-delivery of 5-azacytidine in zein protein nanoparticles with stem cells for cardiac function restoration

Sharma, Vineeta; Dash, Sanat Kumar; Manhas, Amit; Radhakrishnan, Janani; Jagavelu, Kumaravelu; Verma, Rama Shanker

doi:10.1016/j.ijpharm.2021.120673

Cited by 15 publications

(9 citation statements)

References 62 publications

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“…160 In addition, the efficacy of injecting alginate-based hydrogels accompanied by 5-azacytidine and zein protein NP was reported to diminish unfavorable cardiac remodeling following the myocardial infarction through the stimulation of mesenchymal stem cell proliferation, migration, and angiogenesis. 33 Zein can be a great candidate for vascular TE due to its notable mechanical strength; the construction of a zein conduit using a rolling method provided a vascular graft with the proper mechanical strength. 161…”

Section: Polypeptide-based Biomaterials For Cardiovascular Tementioning

confidence: 99%

“…Zein is classified as a prolamine protein, which is mostly found in Maize and would be an enticing option for TE owing to its renewability, nonhazardous nature, biodegradability, and biocompatibility. − For cardiac TE, bead free electrospun poly(glycerol sebacate)-zein fibers were employed to construct a cardiac patch, providing a mechanically supporting matrix for the infarcted cardiac tissue to regenerate . In addition, the efficacy of injecting alginate-based hydrogels accompanied by 5-azacytidine and zein protein NP was reported to diminish unfavorable cardiac remodeling following the myocardial infarction through the stimulation of mesenchymal stem cell proliferation, migration, and angiogenesis . Zein can be a great candidate for vascular TE due to its notable mechanical strength; the construction of a zein conduit using a rolling method provided a vascular graft with the proper mechanical strength …”

Section: Polypeptide-based Biomaterials For Cardiovascular Tementioning

confidence: 99%

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Bacterial Cellulose-Based Materials: A Perspective on Cardiovascular Tissue Engineering Applications

Fooladi

Nematollahi

Rabiee

et al. 2023

ACS Biomater. Sci. Eng.

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Today, a wide variety of bio- and nanomaterials have been deployed for cardiovascular tissue engineering (TE), including polymers, metal oxides, graphene/its derivatives, organometallic complexes/composites based on inorganic–organic components, among others. Despite several advantages of these materials with unique mechanical, biological, and electrical properties, some challenges still remain pertaining to their biocompatibility, cytocompatibility, and possible risk factors (e.g., teratogenicity or carcinogenicity), restricting their future clinical applications. Natural polysaccharide- and protein-based (nano)structures with the benefits of biocompatibility, sustainability, biodegradability, and versatility have been exploited in the field of cardiovascular TE focusing on targeted drug delivery, vascular grafts, engineered cardiac muscle, etc. The usage of these natural biomaterials and their residues offers several advantages in terms of environmental aspects such as alleviating emission of greenhouse gases as well as the production of energy as a biomass consumption output. In TE, the development of biodegradable and biocompatible scaffolds with potentially three-dimensional structures, high porosity, and suitable cellular attachment/adhesion still needs to be comprehensively studied. In this context, bacterial cellulose (BC) with high purity, porosity, crystallinity, unique mechanical properties, biocompatibility, high water retention, and excellent elasticity can be considered as promising candidate for cardiovascular TE. However, several challenges/limitations regarding the absence of antimicrobial factors and degradability along with the low yield of production and extensive cultivation times (in large-scale production) still need to be resolved using suitable hybridization/modification strategies and optimization of conditions. The biocompatibility and bioactivity of BC-based materials along with their thermal, mechanical, and chemical stability are crucial aspects in designing TE scaffolds. Herein, cardiovascular TE applications of BC-based materials are deliberated, with a focus on the most recent advancements, important challenges, and future perspectives. Other biomaterials with cardiovascular TE applications and important roles of green nanotechnology in this field of science are covered to better compare and comprehensively review the subject. The application of BC-based materials and the collective roles of such biomaterials in the assembly of sustainable and natural-based scaffolds for cardiovascular TE are discussed.

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Section: Polypeptide-based Biomaterials For Cardiovascular Tementioning

confidence: 99%

Section: Polypeptide-based Biomaterials For Cardiovascular Tementioning

confidence: 99%

Bacterial Cellulose-Based Materials: A Perspective on Cardiovascular Tissue Engineering Applications

Fooladi

Nematollahi

Rabiee

et al. 2023

ACS Biomater. Sci. Eng.

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“…Researchers coated the cell membrane of MSCs on PLGA particles loaded with MSCs secretions to create a particle called synMSCs and injected it into mice with acute myocardial infarction (AMI), and found that synMSCs-treated mice were able to express MHC I molecules that allowed them to avoid allogeneic recognition by the immune system, thereby modulating the body’s innate immune response and enhancing their attachment to cardiomyocytes and promoting their survival by secreting adhesion factors (such as SDF1) ( Luo et al, 2017 ). Vineeta Sharma and his colleagues found that 5-Aza loaded protein nanoparticle with MSCs encapsulated hydrogels may support in vitro MSC proliferation, migration and angiogenesis in rat MI model, and the hydrogels could alleviate ventricular remodeling after myocardial infarction by secreting immunoregulatory factors ( Sharma et al, 2021 ). The addition of MSCs -derived exosomes to alginate hydrogel revealed that the delivery of exosomes incorporated in alginate hydrogel (sEVs-Gel) increased residence time in the heart, and promoted the polarization of macrophages from M1 phenotype to M2 phenotype more than exosomes treatment alone, promoted scar tissue repair and vascular regeneration ( Lv et al, 2019 ).…”

Section: Immunomodulation Of Mscs In Cardiovascular Diseasementioning

confidence: 99%

Mesenchymal Stem Cell Immunomodulation: A Novel Intervention Mechanism in Cardiovascular Disease

Wang

Yan

et al. 2022

Front. Cell Dev. Biol.

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Mesenchymal stem cells (MSCs) are the member of multipotency stem cells, which possess the capacity for self-renewal and multi-directional differentiation, and have several characteristics, including multi-lineage differentiation potential and immune regulation, which make them a promising source for cell therapy in inflammation, immune diseases, and organ transplantation. In recent years, MSCs have been described as a novel therapeutic strategy for the treatment of cardiovascular diseases because they are potent modulators of immune system with the ability to modulating immune cell subsets, coordinating local and systemic innate and adaptive immune responses, thereby enabling the formation of a stable inflammatory microenvironment in damaged cardiac tissues. In this review, the immunoregulatory characteristics and potential mechanisms of MSCs are sorted out, the effect of these MSCs on immune cells is emphasized, and finally the application of this mechanism in the treatment of cardiovascular diseases is described to provide help for clinical application.

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“…Among various patches/scaffolds, hydrogels undergoing a sol–gel transition could minimally invasively deliver bioactive cargos such as cells and genes to the infarcted region but without being washed out during continuous expansion and contraction of the heart. , Hydrogels adaptive to infarcted heart tissue can not only offer mechanical support and electrical conductivity for similar functional property to that of native heart, but also prepare an extracellular matrix (ECM)-like microenvironment for cardiac repair. − Moreover, since continuous post-MI processes involve ventricular remodeling and myofibroblast proliferation, functional hydrogels, particularly with an ability of inflammation attenuation, neovascularization and structural reinforcement, are expected to repair irreversibly damaged tissues after MI . For example, the injectable hydrogels composed of hyaluronic acid (HA), collagen, gelatin, agarose, chitosan, alginate or poly( N -isopropylacrylamide) can recompense for inadequate mechanical strength of impaired cardiac tissues according to Laplace’s law, thereby rebuilding left ventricular (LV) cavity shape and size .…”

Section: Introductionmentioning

confidence: 99%

Insight into Heart-Tailored Architectures of Hydrogel to Restore Cardiac Functions after Myocardial Infarction

et al. 2022

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With permanent heart muscle injury or death, myocardial infarction (MI) is complicated by inflammatory, proliferation and remodeling phases from both the early ischemic period and subsequent infarct expansion. Though in situ re-establishment of blood flow to the infarct zone and delays of the ventricular remodeling process are current treatment options of MI, they fail to address massive loss of viable cardiomyocytes while transplanting stem cells to regenerate heart is hindered by their poor retention in the infarct bed. Equipped with heart-specific mimicry and extracellular matrix (ECM)-like functionality on the network structure, hydrogels leveraging tissue-matching biomechanics and biocompatibility can mechanically constrain the infarct and act as localized transport of bioactive ingredients to refresh the dysfunctional heart under the constant cyclic stress. Given diverse characteristics of hydrogel including conductivity, anisotropy, adhesiveness, biodegradability, self-healing and mechanical properties driving local cardiac repair, we aim to investigate and conclude the dynamic balance between ordered architectures of hydrogels and the post-MI pathological milieu. Additionally, our review summarizes advantages of heart-tailored architectures of hydrogels in cardiac repair following MI. Finally, we propose challenges and prospects in clinical translation of hydrogels to draw theoretical guidance on cardiac repair and regeneration after MI.

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Injectable hydrogel for co-delivery of 5-azacytidine in zein protein nanoparticles with stem cells for cardiac function restoration

Cited by 15 publications

References 62 publications

Bacterial Cellulose-Based Materials: A Perspective on Cardiovascular Tissue Engineering Applications

Bacterial Cellulose-Based Materials: A Perspective on Cardiovascular Tissue Engineering Applications

Mesenchymal Stem Cell Immunomodulation: A Novel Intervention Mechanism in Cardiovascular Disease

Insight into Heart-Tailored Architectures of Hydrogel to Restore Cardiac Functions after Myocardial Infarction

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