Inhibitory Potential of Ethanol Extract of Detam 1 Soybean (Glycine max) Seed and Jati Belanda (Guazuma ulmifolia) Leaves on Adipogenesis and Obesity Models in 3T3-L1 Cell Line

Hidayat, Meilinah; Soeng, Sylvia; Prahastuti, Sijani; Erawijantari, Pande Putu; Widowati, Wahyu

doi:10.9734/jsrr/2015/16273

Cited by 7 publications

(13 citation statements)

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“…Kandungan tanin daun jati belanda diduga dapat menghambat pembentukan jaringan adipose. 14 Ekstrak air jati belanda mempunyai daya hambat lebih kuat terhadap enzim lipase daripada ekstrak etanolnya. 15 Kemuning mempunyai mekanisme penghambatan enzim lipase dengan mengikat enzim tersebut.…”

Section: Pembahasanunclassified

Pengaruh Jamu Obesitas terhadap Indeks Massa Tubuh, Lingkar Perut, dan Lingkar Lengan dibandingkan dengan Orlistat dan Evaluasi Keamanannya

Ardiyanto¹

2018

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Obesity could be a risk factor for other diseases, especially degenerative diseases. The prevalence of obesity is beginning to elevate at developing countries. Indonesia has some traditional ingredients that can be used for alternatives in obesity problems. But there isnot valid evidence for the efficacy and safety of traditional herbs. This study was conducted to evaluate efficacy of Jamu formulation in comparison with orlistat and evaluate its safety. Jamu formula contains Guazuma ulmifolia dried leaves, Murraya paniculata dried leaves, Rheum officinale dried roots, and Sonchus arvensis dried leaves. The present study was an randomized clinical trial open label undertaken in 242 subjects of obesity for 56 days. The Jamu group received a glass twice daily and orlistat group received a capsule twice daily. Efficacy was assessed by the changes in body mass index, waist and mid-upper arm circumference. Safety was evaluated by SGOT, SGPT, ureum, dan creatinine. There was a significant decrease (p<0,05) in body mass index, waist and mid-upper arm circumference after intervention in both group. Changes of those parameters were not significantly different (p>0,05) between both groups. No significant alteration was seen in most of the SGOT, SGPT, ureum, dan creatinine in Jamu formula groups. This study showed Jamu formula was an equally effective, but safe alternative for obesity management. Abstrak Obesitas dapat menjadi faktor resiko penyakit lain khususnya penyakit degeneratif. Prevalensi obesitas mulai mengalamai peningkatan di negara berkembang. Indonesia memiliki beberapa ramuan tradisional yang dapat digunakan untuk alternatif dalam masalah obesitas. Namun belum ada bukti yang kuat mengenai khasiat dan keamanan dari ramuan tradisional yang ada. Tujuan penelitian ini untuk mengetahui manfaat dan keamanan ramuan jamu yang terdiri dari simplisia daun jati belanda, daun kemuning, akar kelembak, dan daun tempuyung dibandingkan orlistat. Penelitian ini menggunakan randomized clinical trial (RCT) open label dengan 242 subyek selama 56 hari.. Kelompok jamu diberikan segelas jamu 2 kali sehari, sedangkan kelompok orlistat diberikan satu kapsul sehari dua kali. Parameter yang digunakan untuk mengevaluasi khasiat formula jamu dan orlistat adalah indeks masa tubuh (IMT), lingkar perut (LP), lingkar lengan atas (LLA). Sedangkan penilaian keamanan digunakan parameter SGOT, SGPT, BUN, dan kreatinin. Pemberian jamu dan orlistat dapat menurunkan IMT, LP, LLA secara bermakna (p<0,05) jika dibandingkan dengan hari ke-0. Penurunan IMT, LP, LLA kelompok jamu dibandingkan orlistat dapat dikatakan setara (p>0,05). Kelompok jamu menujukkan nilai SGOT, SGPT, ureum, dan kreatinin dalam rentang normal. Penelitian ini menunjukkan bahwa ramuan jamu obesitas secara klinis, khasiatnya sebanding dengan orlistat dan aman setelah intervensi selama 56 hari.

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Section: Pembahasanunclassified

Pengaruh Jamu Obesitas terhadap Indeks Massa Tubuh, Lingkar Perut, dan Lingkar Lengan dibandingkan dengan Orlistat dan Evaluasi Keamanannya

Ardiyanto¹

2018

bpk

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“…6 Kandungan kimia daun Jati Belanda berupa tanin, alkaloid, musilago, saponin, beta-sitosterol, dan flavonoid berperan dalam menghambat penyerapan lipid sehingga kadar lipid dalam darah akan menurun. Selain itu, tanin memiliki aktivitas sebagai antioksidan dan antiinflamasi yang menghambat oksidasi lemak dan pembentukan foam cell.…”

Section: Gambar 2 Efek Kandungan Daun Jati Belanda Dalam Menghambat Punclassified

The Effect of Jati Belanda Leaves (Guazuma ulmifolia Lamk.) Ethanol Extract on Microscopic Features of Atherosclerotic Animal Model’s Aorta

Permana¹,

Azaria²,

Rosnaeni³

2016

Journal of Medicine and Health

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“…SCD1 inhibition in fully differentiated 3T3-L1 adipocytes reported to down -regulated the expression of several genes involved in triacylglycerol (TAG) biosynthesis (Ralston et al, 2014), which is important factor in increasing cell volume of adipocytes (Kim et al, 2012). Previous study has demonstrated that EEJB and EEDS were able to decrease cholesterol and triglyceride levels in 3T3-L1 cells (Hidayat et al, 2015a), these might strongly related to ability of EEJB and EEDS in suppressing SCD1 mRNA level as shown in this study, since SCD1 inhibition can down-regulate biological pathway and genes associated with triglyceride synthesis (Ralston et al, 2014).…”

Section: C/ebp Is One Of Adipogenic Transcription Factors Thatmentioning

confidence: 99%

“…The insulin medium was replaced again and more than 80% cells were differentiated after 7 days. The adipocytes were observed using microscope (Hidayat et al, 2015a;Huang et al, 2006).…”

Section: Adipogenesis Inductionmentioning

confidence: 99%

“…Guazuma ulmifolia Lam., known as "Jati belanda" in Indonesia has been popularly used as therapeutics for hypercholesterolemic, gastro-intestinal disorder, diabetes mellitus, and to reduce weight in some parts of the world (Feltrin et al, 2012;Magos et al, 2008). Our previous study demonstrated that ethanol extract of Detam 1 soybean seeds and ethanol extract of Jati belanda leaves have potential as anti-obesity, it inhibited weight gain in male Wistar rat and possessed inhibitory activities toward glucose-6-phosphate dehydrogenase (G6PD), triglyceride (TG) and cholesterol (CHOL) in 3T3-L1 cell line (Hidayat et al, 2015a;Hidayat et al, 2015b).…”

Section: Introductionmentioning

confidence: 99%

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Modulation of adipogenesis-related gene expression by ethanol extracts of Detam 1 soybean and Jati belanda leaf in 3T3-L1 cells

Hidayat

Prahastuti

Fauziah³

et al. 2016

Bangladesh J Pharmacol

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In this study, we evaluated the effects of ethanol extracts of Detam 1 soybean, Jati belanda leaf, and the combination toward expression of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), and stearoyl-CoA desaturase 1 (SCD1) genes in 3T3-L1 cells as anti-adipogenesis and anti-obesity. The differentiation of 3T3-L1 cells into adipocyte was conducted using induction medium consist of Dulbecco's Modified Eagle's Medium, 3-isobutyl-1-methylxanthine, insulin, dexamethasone, and fetal bovine serum. The expression of PPARγ, C/EBPα, and SCD1 gene was measured using real-time quantitative polymerase chain reaction (qPCR). Ethanol extract of Jati belanda at a concentration of 50 μg/mL was most effective to reduce PPARγ, C/EBPα, and SCD1 gene expression in 3T3-L1 cells. Ethanol extract of Detam 1 soybean failed to reduce PPARγ gene expression, whilst in the concentration of 50 μg/mL it was able to significantly reduce the C/EBPα and SCD1 gene expression. Both ethanol extracts of Detam 1 soybean and Jati belanda have potential as anti-adipogenesis and anti-obesity by suppressing adipogenesis-related gene expression, particularly C/EBPα and SCD1.Video Clip:<a href="https://youtube.com/v/UWv9nChmF2Y">Maintaining 3T3-L1 cell culture</a>: 5 min 15 sec

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Inhibitory Potential of Ethanol Extract of Detam 1 Soybean (Glycine max) Seed and Jati Belanda (Guazuma ulmifolia) Leaves on Adipogenesis and Obesity Models in 3T3-L1 Cell Line

Cited by 7 publications

References 0 publications

Pengaruh Jamu Obesitas terhadap Indeks Massa Tubuh, Lingkar Perut, dan Lingkar Lengan dibandingkan dengan Orlistat dan Evaluasi Keamanannya

Pengaruh Jamu Obesitas terhadap Indeks Massa Tubuh, Lingkar Perut, dan Lingkar Lengan dibandingkan dengan Orlistat dan Evaluasi Keamanannya

The Effect of Jati Belanda Leaves (Guazuma ulmifolia Lamk.) Ethanol Extract on Microscopic Features of Atherosclerotic Animal Model’s Aorta

Modulation of adipogenesis-related gene expression by ethanol extracts of Detam 1 soybean and Jati belanda leaf in 3T3-L1 cells

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