Inhibitory mechanism of 6-Pentyl-2H-pyran-2-one secreted by Trichoderma atroviride T2 against Cylindrocarpon destructans

Jin, Xin; Guo, Liwei; Jin, Baocheng; Zhu, Shusheng; Mei, Xinyue; Wu, Jiaqing; Liu, Tao; He, Xiahong

doi:10.1016/j.pestbp.2020.104683

Cited by 23 publications

(27 citation statements)

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“…Transcriptome analysis was performed to determine the changes in genes according to Jin’s method . The total RNA of all samples was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA).…”

Section: Methodsmentioning

confidence: 99%

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Allyl Isothiocyanate in the Volatiles of Brassica juncea Inhibits the Growth of Root Rot Pathogens of Panax notoginseng by Inducing the Accumulation of ROS

Liu

et al. 2021

J. Agric. Food Chem.

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The cultivation of Panax notoginseng is often seriously hindered by root rot disease caused by the accumulation of soil-borne pathogens. Here, the inhibitory activity of Brassica juncea volatiles on P. notoginseng root rot pathogens was assessed and compounds in volatiles were identified. Furthermore, the antimicrobial activity and mechanism of allyl isothiocyanate (AITC) were deciphered by integrated transcriptome and metabolome analyses. The volatiles of B. juncea showed dose-dependent antimicrobial activity against root rot pathogens. AITC, identified as the main volatile compound, not only significantly inhibited pathogen growth in vitro but also suppressed root rot disease in the field. Integrated transcriptomic and metabolomics analysis revealed that AITC inhibited Fusarium solani by interfering with energy production and induced the accumulation of ROS by decreasing the content of glutathione (GSH). In summary, B. juncea releases AITC to inhibit soil-borne pathogens and could be used as a rotation crop or soil fumigant to alleviate root rot disease.

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Section: Methodsmentioning

confidence: 99%

“…Transcriptome analysis was performed to determine the changes in genes according to Jin's method. 23 The total RNA of all samples was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA). The cDNA was enriched to construct the cDNA library, which was sequenced on the Illumina NovaSeq 6000 platform.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Allyl Isothiocyanate in the Volatiles of Brassica juncea Inhibits the Growth of Root Rot Pathogens of Panax notoginseng by Inducing the Accumulation of ROS

Liu

et al. 2021

J. Agric. Food Chem.

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“…For example, the pyrone, 6-pentyl-2H-pyran-2-one (6-PP) has been found to be produced by several Trichoderma species, including T. atroviride (Garnica-Vergara et al, 2016 ), T harzianum (Rubio et al, 2009 ), T. koningii (Simon et al, 1988 ) and T. viride (Jeleń et al, 2014 ). This metabolite showed a significant antifungal effect against the ascomycete Cylindrocarpon destructans , the causal agent of root rot in Panax notoginseng (Chinese ginseng) (Jin et al, 2020 ). Similarly, evaluation of 6-PP at high concentrations showed significant mycelial growth inhibition of Fusarium culmorum in vitro (Jeleń et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolites derived from fungi and bacteria suppress in vitro growth of Gnomoniopsis smithogilvyi, a major threat to the global chestnut industry

2022

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Introduction Chestnut rot caused by the fungus Gnomoniopsis smithogilvyi is a disease present in the world’s major chestnut growing regions. The disease is considered a significant threat to the global production of nuts from the sweet chestnut (Castanea sativa). Conventional fungicides provide some control, but little is known about the potential of biological control agents (BCAs) as alternatives to manage the disease. Objective Evaluate whether formulated BCAs and their secreted metabolites inhibit the in vitro growth of G. smithogilvyi. Methods The antifungal potential of BCAs was assessed against the pathogen through an inverted plate assay for volatile compounds (VOCs), a diffusion assay for non-volatile compounds (nVOCs) and in dual culture. Methanolic extracts of nVOCs from the solid medium were further evaluated for their effect on conidia germination and were screened through an LC–MS-based approach for antifungal metabolites. Results Isolates of Trichoderma spp., derived from the BCAs, significantly suppressed the pathogen through the production of VOCs and nVOCs. The BCA from which Bacillus subtilis was isolated was more effective in growth inhibition through the production of nVOCs. The LC–MS based metabolomics on the nVOCs derived from the BCAs showed the presence of several antifungal compounds. Conclusion The results show that G. smithogilvyi can be effectively controlled by the BCAs tested and that their use may provide a more ecological alternative for managing chestnut rot. The in vitro analysis should now be expanded to the field to assess the effectiveness of these alternatives for chestnut rot management.

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“…Regarding nutrient deprivation, vacuoles are degradative organelles, and autophagy in filamentous fungi is accompanied by the enlargement and degradation of vacuoles in the hyphae, with autophagosomes being found in the vacuoles [ 84 ]. Further, a study on Cylindrocarpon destructans showed that the active substance of Trichoderma atroviride T2 induced autophagy in pathogenic fungi [ 85 ]. In our study, under stress caused by B. methylotrophicus NJ13’s SMs, autophagy may have occurred in strain CBLJ-3.…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Provides Insights into the Mechanisms Underlying Ilyonectria robusta Responding to Secondary Metabolites of Bacillus methylotrophicus NJ13

Liu

et al. 2022

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(1) Background: Ilyonectria robusta can cause ginseng to suffer from rusty root rot. Secondary metabolites (SMs) produced by Bacillus methylotrophicus NJ13 can inhibit the mycelial growth of I. robusta. However, the molecular mechanism of the inhibition and response remains unclear. (2) Methods: Through an in vitro trial, the effect of B. methylotrophicus NJ13’s SMs on the hyphae and conidia of I. robusta was determined. The change in the physiological function of I. robusta was evaluated in response to NJ13’s SMs by measuring the electrical conductivity, malondialdehyde (MDA) content, and glucose content. The molecular interaction mechanism of I. robusta’s response to NJ13’s SMs was analyzed by using transcriptome sequencing. (3) Results: NJ13’s SMs exhibited antifungal activity against I. robusta: namely, the hyphae swelled and branched abnormally, and their inclusions leaked out due to changes in the cell membrane permeability and the peroxidation level; the EC50 value was 1.21% (v/v). In transcripts at 4 dpi and 7 dpi, the number of differentially expressed genes (DEGs) (|log2(fold change)| > 1, p adj ≤ 0.05) was 1960 and 354, respectively. NJ13’s SMs affected the glucose metabolism pathway, and the sugar-transporter-related genes were downregulated, which are utilized by I. robusta for energy production. The cell wall structure of I. robusta was disrupted, and chitin-synthase-related genes were downregulated. (4) Conclusions: A new dataset of functional responses of the ginseng pathogenic fungus I. robusta was obtained. The results will benefit the development of targeted biological fungicides for I. robusta and the study of the molecular mechanisms of interaction between biocontrol bacteria and phytopathogenic fungi.

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Inhibitory mechanism of 6-Pentyl-2H-pyran-2-one secreted by Trichoderma atroviride T2 against Cylindrocarpon destructans

Cited by 23 publications

References 56 publications

Allyl Isothiocyanate in the Volatiles of Brassica juncea Inhibits the Growth of Root Rot Pathogens of Panax notoginseng by Inducing the Accumulation of ROS

Allyl Isothiocyanate in the Volatiles of Brassica juncea Inhibits the Growth of Root Rot Pathogens of Panax notoginseng by Inducing the Accumulation of ROS

Metabolites derived from fungi and bacteria suppress in vitro growth of Gnomoniopsis smithogilvyi, a major threat to the global chestnut industry

RNA-Seq Provides Insights into the Mechanisms Underlying Ilyonectria robusta Responding to Secondary Metabolites of Bacillus methylotrophicus NJ13

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