Allyl Isothiocyanate in the Volatiles of <i>Brassica juncea</i> Inhibits the Growth of Root Rot Pathogens of <i>Panax notoginseng</i> by Inducing the Accumulation of ROS

Liu, Haijiao; Wu, Jiaqing; Su, Yingwei; Li, Yingbin; Zuo, Denghong; Liu, Hongbin; Liu, Yixiang; Mei, Xinyue; Huang, Huichuan; Yang, Min; Zhu, Shusheng

doi:10.1021/acs.jafc.1c05225

Cited by 19 publications

(31 citation statements)

References 47 publications

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“…aureofaciens SPS-41 can emit VOCs to control Ceratocystis fimbriata in postharvest sweet potato. Recent studies showed that allyl isothiocyanate in the volatiles of Brassica juncea inhibits the growth of root rot pathogens in Panax notoginseng by inducing the accumulation of reactive oxygen species (ROS) ( Zhang et al, 2019 ; Liu H. J. et al, 2021 ). Thus, the exploitation of the biofumigation properties of microbial VOCs promises to be a novel and ecologically friendly approach for the control of soil-borne diseases.…”

Section: Introductionmentioning

confidence: 99%

Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae

Kong

Wang

et al. 2022

Front. Microbiol.

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Volatile organic compounds (VOCs) produced by microorganisms are considered promising environmental-safety fumigants for controlling soil-borne diseases. Verticillium dahliae, a notorious fungal pathogen, causes economically important wilt diseases in agriculture and forestry industries. Here, we determined the antifungal activity of VOCs produced by Trichoderma koningiopsis T2. The VOCs from T. koningiopsis T2 were trapped by solid-phase microextraction (SPME) and tentatively identified through gas chromatography–mass spectrometry (GC/MS). The microsclerotia formation, cell wall-degrading enzymes and melanin synthesis of V. dahliae exposed to the VOC mixtures and selected single standards were examined. The results showed that the VOCs produced by strain T2 significantly inhibited the growth of V. dahliae mycelium and reduced the severity of Verticillium wilt in tobacco and cotton. Six individual compounds were identified in the volatilome of T. koningiopsis T2, and the dominant compounds were 3-octanone, 3-methyl-1-butanol, butanoic acid ethyl ester and 2-hexyl-furan. The VOCs of strain T2 exert a significant inhibitory effect on microsclerotia formation and decreased the activities of pectin lyase and endo-β-1,4-glucanase in V. dahliae. VOCs also downregulated the VdT3HR, VdT4HR, and VdSCD genes related to melanin synthesis by 29. 41-, 10. 49-, and 3.11-fold, respectively. Therefore, T. koningiopsis T2 has potential as a promising biofumigant for the biocontrol of Verticillium wilt disease.

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Section: Introductionmentioning

confidence: 99%

Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae

Kong

Wang

et al. 2022

Front. Microbiol.

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“…AI increased intracellular ROS levels by interfering with glutathione metabolism, thereby achieving the inhibition of root rot pathogens of Panax notoginseng. 47 Moreover, linalool released by strawberry induced ROS generation in B. cinerea cells, accompanied by increased production of the ROS scavenger pyruvate. 57 Interestingly, this MOA seemed to also exist in eugenol but not in 1-octene-3-ol and 2-octanone.…”

Section: ■ Discussionmentioning

confidence: 99%

“…DCFH-DA staining was used to detect intracellular ROS production. 47 Mycelia were treated with T2H of 20 μL/mL for 24 h as described in the mycelial biomass assay, collected, and stained with 10 μM DCFH-DA in the dark for 30 min at 28 °C. The concentration of T2H was adjusted to 0 μL/mL as the control.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Green Leaf Volatile Trans-2-Hexenal Inhibits the Growth of Fusarium graminearum by Inducing Membrane Damage, ROS Accumulation, and Cell Dysfunction

Wang

Zhu

et al. 2022

J. Agric. Food Chem.

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Fusarium graminearum, the main agent of Fusarium head blight (FHB), can cause serious yield loss and secrete mycotoxins to contaminate grain. Here, the biological activity of trans-2-hexenal (T2H) against F. graminearum was determined and its mode of action (MOA) was investigated. Furthermore, surface plasmon resonance with liquid chromatography–tandem mass spectrometry (SPR-LC–MS/MS), bioinformatic analysis, and gene knockout technique were combined to identify the binding proteins of T2H in F. graminearum cells. T2H exhibited satisfactory inhibitory activity against F. graminearum in vitro. Good lipophilicity greatly enhanced the affinity of T2H to F. graminearum mycelia and further caused membrane damage. The FgTRR (thioredoxin reductase) gene negatively regulates the sensitivity of F. graminearum to T2H by reducing the generation of reactive oxygen species (ROS) induced by T2H. Two mutant strains with FgSLX1 (structure-specific endonuclease subunit) and FgCOPB (coatomer subunit β) genes knockout showed decreased sensitivity to T2H, suggesting that these two genes may be involved in the antimicrobial activity of T2H. Taken together, T2H can inhibit F. graminearum growth by multiple MOAs and can be used as a biofumigant to control the occurrence of FHB in the field.

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“…Root exudates, including flavone phenolic alcohols, terpenes, isothiocyanates, and glucosinolates ( Ren et al, 2003 ; Kliebenstein, 2009 ; Wu et al, 2009 ; Sanchez-Vallet et al, 2010 ; Cruz et al, 2012 ; Liu H. et al, 2021 ), improved plant growth and weakened soil-borne pathogens. Among those compounds, terpenes are hydrocarbons that contain one or more carbon–carbon double bonds and share the same elementary unit of isoprene (2-methyl-1,4-butadiene), with additional pollinator-attractive properties and strong antimicrobial activity against microorganisms ( Dalleau et al, 2008 ; Pandey et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Terpenes Suppressed the Infection Process of Phytophthora in Fennel-Pepper Intercropping System

et al. 2022

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The interactions between non-host roots and pathogens may be key to the inhibition of soilborne pathogens in intercropping systems. Fennel (Foeniculum vulgare) can be intercropped with a wide range of other plants to inhibit soilborne pathogens in biodiversity cultivation. However, the key compounds of fennel root exudates involved in the interactions between fennel roots and pathogens are still unknown. Here, a greenhouse experiment confirmed that intercropping with fennel suppressed pepper (Capsicum annuum) blight disease caused by Phytophthora capsici. Experimentally, the roots and root exudates of fennel can effectively interfere with the infection process of P. capsici at rhizosphere soil concentrations by attracting zoospores and inhibiting the motility of the zoospores and germination of the cystospores. Five terpene compounds (D-limonene, estragole, anethole, gamma-terpenes, and beta-myrcene) that were identified in the fennel rhizosphere soil and root exudates were found to interfere with P. capsica infection. D-limonene was associated with positive chemotaxis with zoospores, and a mixture of the five terpene compounds showed a strong synergistic effect on the infection process of P. capsici, especially for zoospore rupture. Furthermore, the five terpene compounds can induce the accumulation of reactive oxygen species (ROS), especially anethole, in hyphae. ROS accumulation may be one of the antimicrobial mechanisms of terpene compounds. Above all, we proposed that terpene compounds secreted from fennel root play a key role in Phytophthora disease suppression in this intercropping system.

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Allyl Isothiocyanate in the Volatiles of Brassica juncea Inhibits the Growth of Root Rot Pathogens of Panax notoginseng by Inducing the Accumulation of ROS

Cited by 19 publications

References 47 publications

Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae

Antifungal effects of volatile organic compounds produced by Trichoderma koningiopsis T2 against Verticillium dahliae

Green Leaf Volatile Trans-2-Hexenal Inhibits the Growth of Fusarium graminearum by Inducing Membrane Damage, ROS Accumulation, and Cell Dysfunction

Antimicrobial Terpenes Suppressed the Infection Process of Phytophthora in Fennel-Pepper Intercropping System

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