Inhibitory effects of flavonoids from Abelmoschus manihot flowers on triglyceride accumulation in 3T3-L1 adipocytes

An, Yonghui; Zhang, Yi; Li, Chunmei; Qian, Qian; He, Wei; Wang, Tao

doi:10.1016/j.fitote.2011.01.010

Cited by 36 publications

(46 citation statements)

References 26 publications

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“…The results showed that the viability of 3 T3-L1 cells in the presence of 6 was not decreased. Therefore, the experiments in this study were performed at compound concentrations of 10, 25 and 50 μg mL −1 (Fig.…”

Section: Cell Viabilitymentioning

confidence: 91%

“…To evaluate the effects of the phlorotannins compounds isolated from E. bicyclis on differentiation of 3 T3-L1 adiopocytes, we measured lipid accumulation by Oil Red O staining. The inhibitory effects of 6,6 ′ -bieckol were further confirmed by downregulation of the expression of C/EBP , SREBP-1 and PPAR (mRNA and protein), fatty acid synthase (FAS) and acyl-coA carboxylase (ACC) (mRNA).…”

Section: Introductionmentioning

confidence: 89%

“…′ -bieckol on expression of PPAR , C/EBP and SREBP-1c during 3T3-L1 differentiation To gain a better understanding of the molecular mechanisms underlying the observed anti-adipogenic effects, we conducted RT-PCR and western blotting analysis to examine the influence of 6,6 ′ -bieckol on the expression of key transcription factors (Figs 4 and 5). Compared to the differentiated adipocytes in the control cultures, treatment with 6,6…”

Section: Suppressive Effect Of 66mentioning

confidence: 99%

“…5 Adipocytes, also known as fat cells or lipocytes, are the cells that primarily compose adipose tissue; they are associated with lipid homeostasis and energy balance. 6 3 T3-L1 cells have been previously reported as a reliable in vitro model for the evaluation of adipocyte differentiation 7 and therefore have been widely used to evaluate potential anti-obesity strategies that target inhibition of adipocyte differentiation. Adipocyte differentiation is regulated by a number of transcription factors such as peroxisome proliferator-activated receptor (PPAR ), sterol regulatory element binding protein (SREBP-1c) and CCATT/enhancer binding protein (C/EBP ).…”

Section: Introductionmentioning

confidence: 99%

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6,6′‐Bieckol inhibits adipocyte differentiation through downregulation of adipogenesis and lipogenesis in 3T3‐L1 cells

Kwon

Kim

et al. 2014

J Sci Food Agric

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Section: Cell Viabilitymentioning

confidence: 91%

Section: Introductionmentioning

confidence: 89%

Section: Suppressive Effect Of 66mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

6,6′‐Bieckol inhibits adipocyte differentiation through downregulation of adipogenesis and lipogenesis in 3T3‐L1 cells

Kwon

Kim

et al. 2014

J Sci Food Agric

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“…Some natural products and plant extracts were found to inhibit the adipogenesis of 3T3-L1 cells [9,10].…”

Section: Introductionmentioning

confidence: 99%

The Inhibitory Effect of Different Solvents Extracts from Walnut Shell (<i>Juglans regia </i><i>L.</i>) on Pancreatic Lipase and Adipogenesis of 3T3-L1 Preadipocytes

Yang¹,

Chen²,

Zhao³

et al. 2014

JFNR

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In this article, the effect of the solvents (water, methanol, ethanol, chloroform, N-butanol and ethyl acetate) on the extraction yields (EY), total flavonoids content (TFC), total phenols content (TPC) form walnut shell and the inhibitory effect of extracts on pancreatic lipase were analysed. The kinetics of enzyme inhibition and was investigated. The inhibitory effect of extracts on adipogenesis of 3T3-L1 preadipocytes was also investigated. The highest EY, TFC and TPC were achieved with N-butanol (4.54%), Ethyl acetate (80.40 mg QEs/g extract) and ethyl acetate (200.40 mg GAE/g extract). The order of inhibitory activity was methanol extract (ME) > ethyl acetate extract (EAE) > ethanol extract (EE) > water extract (WE) > N-butanol extract (NBE) > chloroform extract (CE), the inhibition ratios were 82.51%, 73.13%, 70.49%, 69.42%, 65.57% and 60.66%, respectively under the concentration of 500 μg/mL. The IC 50 values of WE, ME, EE, CE, NBE and EAE were 371.35, 308.91, 407.57, 196.50, 247.79 and 254.27 μg/mL respectively. The inhibitory types of walnut shell extracts were noncompetitive. All the extracts were non-toxic to 3T3-L1 preadipocytes. The ME, EE, CE, NBE and EAE have inhibitory effect on triglyceride accumulation of 3T3-L1 cells.

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Metabolic profiles of the Flos Abelmoschus manihot extract by intestinal bacteria from the normal and CKD model rats based on UPLC‐Q‐TOF/MS

Jiang

et al. 2016

Biomedical Chromatography

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Flos Abelmoschus manihot is a traditional herbal medicine widely used in clinical practice to tackle chronic kidney disease (CKD) for thousands of years. Nowadays, many studies indicate that gut bacteria are closely related to the progression of CKD and CKD-related complications. In this study, a UPLC-Q-TOF/MS method coupled with the MetaboLynx™ software was established and successfully applied to investigate the metabolites and metabolic profile of Flos A. manihot extract by intestinal bacteria from normal and CKD rats. Eight parent components and eight metabolites were characterized by their protonated ions. Among these compounds, 15 were detected in the two group samples while M16 was only determined in the CKD model samples. Compared with the quercetin-type glycosides, fewer myricetin-type and gossypetin-type metabolites were obtained in the two group samples. These metabolites suggested that deglycosylation and methylation are the major metabolic pathways of Flos A. manihot extract. Few differences of metabolite classes were observed in the two group samples. However, the concentrations of aglycones such as quercetin, myricetin and gossypetin in the normal samples were notably higher than those in the CKD model samples. The results are important in unravelling the pharmacological effects of A. manihot and clarifying its mechanism of action in vivo.

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Inhibitory effects of flavonoids from Abelmoschus manihot flowers on triglyceride accumulation in 3T3-L1 adipocytes

Cited by 36 publications

References 26 publications

6,6′‐Bieckol inhibits adipocyte differentiation through downregulation of adipogenesis and lipogenesis in 3T3‐L1 cells

6,6′‐Bieckol inhibits adipocyte differentiation through downregulation of adipogenesis and lipogenesis in 3T3‐L1 cells

The Inhibitory Effect of Different Solvents Extracts from Walnut Shell (<i>Juglans regia </i><i>L.</i>) on Pancreatic Lipase and Adipogenesis of 3T3-L1 Preadipocytes

Metabolic profiles of the Flos Abelmoschus manihot extract by intestinal bacteria from the normal and CKD model rats based on UPLC‐Q‐TOF/MS

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