Inhibitory Effects of Bilirubin on Colonization and Migration of A431 and SK-MEL-3 Skin Cancer Cells Compared with Human Dermal Fibroblasts (HDF)

Background Colon cancer is the third most common cancer and the fourth leading cause of death from cancer. Some parasites are introduced as an antineoplastic agents that can inhibit the progress of some cancers. The aim of this study was to investigate the effect of crude hydatid cyst fluid (HCF) on clone cancer cell line (C26). Methods HCF was isolated from hydatid cysts by syringe, and at the first, its toxicity was obtained by 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Cell cycle analysis and apoptosis were measured by flow cytometer, and also the expression of Bcl-2 Associated X-protein (BAX) and B-cell lymphoma-2 (BCL2) genes was measured by quantitative reverse transcription PCR. Results The amount of apoptosis was increased in B antigen-treated cell lines in comparison with the control group. Also, the expression of BAX was increased in the treated group, while the BCL2 expression was decreased in comparison with the control one. Cell cycle analysis in the antigen-treated group compared to the other groups showed that the cells were more in the G0/G1 phase, as well as in the G2/M phase, and fewer cells were in the synthesis phase. Conclusion Our finding showed that HCF possibly contains active compounds and can limit the growth and development of C26 cell line by reducing or increasing the genes involved in apoptosis and finally the effect on the cell cycle. Graphical Abstract

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“…The number of cycles in all reactions was considered 40 times. In this study, the GAPDH gene was used as a reference gene for normalization [35].…”

Section: Determination Of Expression Of Bax and Bcl2 Genes In Prepare...mentioning

confidence: 99%

In vitro anticancer activity of hydatid cyst fluid on colon cancer cell line (C26)

Motavallihaghi

Tanzadehpanah

Asl

et al. 2023

Egypt J Med Hum Genet

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Ameliorative effects of bilirubin on cell culture model of non-alcoholic fatty liver disease

et al. 2023

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Bilirubin improves renal function by reversing the endoplasmic reticulum stress and inflammation in the kidneys of type 2 diabetic rats fed high-fat diet

Maleki

Nadimi

Vakili

et al. 2023

Chemico-Biological Interactions

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Inhibitory Effects of Bilirubin on Colonization and Migration of A431 and SK-MEL-3 Skin Cancer Cells Compared with Human Dermal Fibroblasts (HDF)

Cited by 4 publications

References 38 publications

In vitro anticancer activity of hydatid cyst fluid on colon cancer cell line (C26)

In vitro anticancer activity of hydatid cyst fluid on colon cancer cell line (C26)

Ameliorative effects of bilirubin on cell culture model of non-alcoholic fatty liver disease

Bilirubin improves renal function by reversing the endoplasmic reticulum stress and inflammation in the kidneys of type 2 diabetic rats fed high-fat diet

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