Inhibitory effect of sodium bicarbonate on the motility of sperm of Japanese eel

Tanaka, Satoru; Zhang, H.; Yamada, Yoshiaki; Okamura, Atsushi; Horie, Noriyuki; Utoh, Tomoko; Mikawa, Naomi; Oka, Hideo; Kurokura, Hisashi

doi:10.1111/j.1095-8649.2002.tb01710.x

Cited by 18 publications

(11 citation statements)

References 29 publications

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“…In a previous study, the authors of the present study reported the inhibitory effect of NaHCO 3 on the initiation of sperm motility in the Japanese eel Anguilla japonica and applied the result to sperm preservation. 8,12 The next step was to find out the role of NaHCO 3 in order to improve the motility of the preserved sperm. In marine teleosts, [pH] i , [K + ] i and [Ca 2+ ] i have been reported as internal factors for the initiation of sperm motility.…”

Section: Discussionmentioning

confidence: 99%

“…Quantification of sperm motility was carried out as reported in the study by Tanaka et al . 12 The following solutions were prepared for the experiments: NaCl, potassium chloride (KCl), CH 3 COONa, CaCl 2 , 2-morpholinoethane-sulfonic acid monohydrate (MES), 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethane-sulfonic acid (HEPES) and TAPS (all from Wako Pure Chemical Industries, Osaka, Japan); NaHCO 3 and NH 4 Cl (Katayama Chemical Industries, Osaka, Japan); and 4-AP (Sigma Chemical Co., St. Louis, MO, USA). Distilled water was used for the dissolution of all reagents.…”

Section: Sperm Samples: Quantification Of Motility and Reagentsmentioning

confidence: 99%

“…In light of this, the inhibitory effect of NaHCO 3 on the initiation of sperm motility in the Japanese eel has been investigated by the authors of the present report and applied to sperm preservation in previous reports. 8,12 The next step was to attempt to discover the fundamental roles of NaHCO 3 in order to improve the motility of the preserved sperm. To investigate the role of NaHCO 3 , various reagents were selected that could regulate the internal factors mentioned above.…”

Section: Introductionmentioning

confidence: 99%

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Role of sodium bicarbonate on the initiation of sperm motility in the Japanese eel

Tanaka¹,

Utoh²,

Yamada³

et al. 2004

Fisheries Sci

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In order to find out the role of sodium bicarbonate (NaHCO 3 ) on the initiation of sperm motility in the Japanese eel Anguilla japonica , interactions were investigated between NaHCO 3 and various reagents (K + channel blocker 4-aminopyridine [4-AP], ammonium chloride [NH 4 Cl], sodium acetate and calcium chloride [CaCl 2 ]) that could regulate internal factors (intracellular K + , intracellular pH [[pH] i ] and intracellular Ca 2 + ) in sperm motility. Contradictory effects of NaHCO 3 were observed (i.e. an inhibitory effect when 4-AP was absent and a promoting effect when 4-AP was present). Sodium bicarbonate inhibited the initiation of sperm motility in the Japanese eel. However, NaHCO 3 restored the motility of immotile sperm that 4-AP inhibited. The inhibitory effect of NaHCO 3 disappeared with the addition of NH 4 Cl, which raised [pH] i , but the promoting effect was not affected by [pH] i . Although NaHCO 3 recovered motility in the presence of 4-AP, this recovery was also observed with the addition of CaCl 2 instead of NaHCO 3 . In the initiation of sperm motility in the Japanese eel, two roles for NaHCO 3 are suggested: an inhibitory role relating to the regulation of [pH] i and a promoting role relating to the uptake of another initiation factor, which could be Ca 2 + .

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Section: Discussionmentioning

confidence: 99%

Section: Sperm Samples: Quantification Of Motility and Reagentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of sodium bicarbonate on the initiation of sperm motility in the Japanese eel

Tanaka¹,

Utoh²,

Yamada³

et al. 2004

Fisheries Sci

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“…New experiments are required using dilution factors as low as 1:1 or 1:2, allowing the maintenance of high concentrated sperm samples in the 250 ll straws. On the other hand, sodium bicarbonate concentrations as high as 75 mM seem to be a critical point, due to its role in controlling the initiation of spermatozoa motility, as has been proven in the Japanese eel (Tanaka et al 2002a(Tanaka et al , 2002b2004).…”

Section: As Wellmentioning

confidence: 93%

Physio-Chemical Characteristics of Seminal Plasma and Development of Media and Methods for the Cryopreservation of European eel Sperm

Asturiano

Pérez

Garzón

et al. 2004

Fish Physiol Biochem

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The high sperm density, together with the short spermatozoa swimming time, makes European eel sperm manipulation and assessment for quality difficult. Two diluting media (K15 and K30) previously designed for Japanese eel sperm were tested. After 24 h, European eel sperm showed significant reduction in the percentage of motile spermatozoa after activation and different motility parameters (VAP, angular velocity; VCL, curvilinear velocity; VSL, straight line velocity; BCF, beating cross frequency), concluding that these media are not suitable to preserve the sperm of this species. After a hormonal treatment to induce spermiation, sperm volume, density and motility were recorded at weekly samplings. The variation of the osmolality (325-330 mOsm kg )1 ), pH (8.4-8.6) and the ionic composition (concentration of Na + , K + , Mg 2+ and Ca 2+ ) of the seminal plasma were registered. Physio-chemical results were related with sperm quality throughout the treatment, to determine which must be the suitable characteristics of one extender for the sperm of this species, and to find the best conditions to obtain suitable cryopreservation media for European eel sperm. K + concentration increased, while Ca 2+ and Mg 2+ concentrations showed a progressive reduction in correlation with the sperm quality improvement. Na + showed a decreasing, but not significant tendency. P1 and P2 freezing media were designed considering the physio-chemical parameters as well as the ionic composition shown by the best quality sperm samples, and then compared with the previously described solutions, TNK and K30. Sperm quality was determined, checking the percentage of motile spermatozoa and motility parameters using computer-assisted sperm analysis (CASA) software. Samples were frozen after dilution (1:5, 1:20, 1:100) in different freezing media supplemented with 10% dimethyl sulfoxide (DMSO). After thawing, samples frozen with low dilution ratio (1:5) in TNK and P1 media showed higher, although not significant, spermatozoa survival (35.5±14.5 and 36.6±6.7%). The addition of L-a-phosphatidylcholine to the media seems to have a positive effect, as reported in the Japanese eel.

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“…In the present study, we will compare the P1 medium (Asturiano et al 2004) and the medium described above (Tanaka et al 2002a). Tanaka et al (2002b) suggested that NaHCO 3 has an inhibitory role on the movement of the Japanese eel sperm, as to trigger the initiation of movement is necessary the influx of H + in the cell, and NaHCO 3 decreases intracellular pH. This decrease in the intracellular pH is produced because intracellular CO 2 (derived from NaHCO 3 ) is converted in HCO À 3 and H + by carbonic anhydrase.…”

Section: Introductionmentioning

confidence: 99%

Effects of pH, Sodium Bicarbonate, Cryoprotectants and Foetal Bovine Serum on the Cryopreservation of European Eel Sperm

Garzón

Peñaranda

Pérez

et al. 2007

Reprod Domestic Animals

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The main objective of the present study was to evaluate the influence of pH and bicarbonate concentration in the activation or inhibition of European eel (Anguilla anguilla) spermatozoa and to evaluate the effect of different cryoprotectants: dimethyl sulphoxide (DMSO), acetamide, ethylene glycol, propanol, glycerol and methanol (MeOH). The effect of these factors was evaluated comparing the percentage of motile cells, the percentage of alive cells (by Hoechst staining) and the spermatozoa morphometry pre- and post-cryopreservation (by computer-assisted morphology analysis). Based on the above findings, three cryoprotectants (DMSO, MeOH and glycerol) were chosen and evaluated in two media (P1 and P1 modified) with different concentrations of NaHCO(3) and in the presence or absence of foetal bovine serum (FBS). The effect of these factors was evaluated comparing the percentage of alive and motile cells post-cryopreservation. DMSO was the cryoprotectant showing better results in relation to the percentage of spermatic alive cells post-freezing and caused a smaller modification of the head spermatozoa morphology. The combination of P1-modified medium with DMSO and containing FBS increased slightly but significantly the percentage of motile spermatozoa post-cryopreservation.

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Inhibitory effect of sodium bicarbonate on the motility of sperm of Japanese eel

Cited by 18 publications

References 29 publications

Role of sodium bicarbonate on the initiation of sperm motility in the Japanese eel

Role of sodium bicarbonate on the initiation of sperm motility in the Japanese eel

Physio-Chemical Characteristics of Seminal Plasma and Development of Media and Methods for the Cryopreservation of European eel Sperm

Effects of pH, Sodium Bicarbonate, Cryoprotectants and Foetal Bovine Serum on the Cryopreservation of European Eel Sperm

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