Role of sodium bicarbonate on the initiation of sperm motility in the Japanese eel

Tanaka, Satoru; Utoh, Tomoko; Yamada, Yoshiaki; Horie, Noriyuki; Okamura, Atsushi; Akazawa, Atsushi; Mikawa, Naomi; Oka, Hideo; Kurokura, Hisashi

doi:10.1111/j.1444-2906.2004.00871.x

Cited by 19 publications

(24 citation statements)

References 26 publications

(80 reference statements)

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“…This decrease should be considered in order to find ways of improvement of the techniques for the cryopreservation of European eel sperm. The way to improve the protocols should consider several aspects as dilution factors (sperm : freezing medium), addition of compounds with friendly effect with the spermatozoa membrane as L‐ α ‐phosphatidylcholine, and variations on the ionic composition of freezing media taken into account for instance the sodium bicarbonate concentrations that could be a critical point due its role controlling the initiation of spermatozoa motility as has been probed in the Japanese eel (Tanaka et al. 2002a,b, 2004).…”

Section: Discussionmentioning

confidence: 99%

Effect of Sperm Cryopreservation on the European Eel Sperm Viability and Spermatozoa Morphology

Asturiano

Marco‐Jiménez

Peñaranda

et al. 2007

Reprod Domestic Animals

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The main objective of the present work was to study the effect of cryopreservation of European eel sperm both on the sperm viability and the spermatozoa head morphology. Spermatozoa morphology was evaluated with computer-assisted morphology analysis after collection in fresh samples, after adding the freezing medium containing dimethyl sulfoxide as cryoprotectant and, finally, after the cryopreservation process and thawing. Cell viability was assessed, in both fresh and thawed samples, by Hoechst 33258 staining. Computer-assisted sperm analysis (CASA) was used to determine the percentage of motile cells and to measure motility parameters in sperm samples. A significant decrease of head perimeter (12.56%) and area (17.90%) was detected from spermatozoa in fresh to thawed samples, indicating that cells do not recover the original size after the cryopreservation process. CASA was used to measure the percentage of motile cells (51.9%) and spermatozoa motility parameters such as curvilinear, straight line and angular path velocities, as well as beating cross frequency. This technique was employed in the fresh sperm samples but proteins present at the freezing medium (L-alpha-phosphatidylcholine) made impossible to use this last technique in thawed samples. When sperm viability was assessed by Hoechst staining, a significant decrease of approximately 15% (73.10 vs 58.26%) of alive spermatozoa was registered from fresh to thawed samples. The percentage of motile cells measured by CASA in fresh samples (51.9%) was lower than the percentage of alive cells determined by Hoechst stainning, suggesting the existence of different batches of spermatozoa in different stages of development, even during the eight to tenth weeks of treatment, when the highest sperm quality was found.

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Section: Discussionmentioning

confidence: 99%

Effect of Sperm Cryopreservation on the European Eel Sperm Viability and Spermatozoa Morphology

Asturiano

Marco‐Jiménez

Peñaranda

et al. 2007

Reprod Domestic Animals

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“…(2002a); Tanaka, Zhang, Yamada, Okamura, Horie, Utoh, Mikawa, Oka and Kurokura (2002b) suggested that NaHCO 3 has an inhibitory role in the Japanese eel spermatozoa motility, as a consequence of its dissociation in some products, which may influence intracellular pH. Two years later, Tanaka, Utoh, Yamada, Horie, Okamura, Akazawa, Mikawa, Oka and Kurokura (2004) suggested that NaHCO 3 has a dual role in the control of spermatozoa motility depending on its dissociation products: one inhibiting role mediated by CO 2 and one activating role mediated by HCO 3 − .…”

Section: Discussionmentioning

confidence: 99%

Effect of different methods for the induction of spermiation on semen quality in European eel

et al. 2005

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Five hormonal treatments with human chorionic gonadotropin (hCG) were tested for the induction of maturation and spermiation in male farmed eels. The main aim was to optimize previously used hormonal treatments to achieve shorter induction treatments, longer spermiation periods and/or higher sperm quality. Fish treated for just 3 weeks (treatment E) or until the onset of spermiation (treatment C) showed the worst results, while the treatment consisting of weekly administration of 1.5 IU hCG g À1 ¢sh (treatment A) induced the highest percentage of spermiating males, the highest number of sperm samples and sperm volumes and densities similar to the rest of the treatments (B: half hormone dosage, or D: biweekly administration). Evaluation of the sperm quality was performed by computer-assisted sperm analysis (CASA), considering the percentage of total motile spermatozoa, the percentage of fast and medium-velocity spermatozoa, as well as di¡erent motility parameters. Sperm samples from A-D groups showed between 44% and 54% motile spermatozoa, and between 10% and 15% fast spermatozoa, while samples from E-treated males showed 0% motile cells. No signi¢cant di¡erences were found in the spermatozoa straight line velocity (VSL), curvilinear velocity (VCL) or the angular velocity (VAP), neither spermatozoa beating cross frequency (BCF) between A^D groups.

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“…In the same study it was shown that pH also regulated sperm motility in puffer fish; in demembranated sperm motility was initiated at a high pH, and terminated at a low pH (Takai & Morisawa, 1995). In the Atlantic croaker (Micropogonias undulatus) and the Japanese eel (Anguilla japonica), the K + channel inhibitor 4-AP inhibited sperm motility (Detweiler & Thomas, 1997;Tanaka et al 2004). In the Japanese eel the inhibitory effect of 4-AP disappeared when the pH i was decreased using Na-acetate, when CO 2 or NaHCO 3 was added, or when the pH from the activation media was lower than 8.2 (Tanaka et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…In the Atlantic croaker (Micropogonias undulatus) and the Japanese eel (Anguilla japonica), the K + channel inhibitor 4-AP inhibited sperm motility (Detweiler & Thomas, 1997;Tanaka et al 2004). In the Japanese eel the inhibitory effect of 4-AP disappeared when the pH i was decreased using Na-acetate, when CO 2 or NaHCO 3 was added, or when the pH from the activation media was lower than 8.2 (Tanaka et al, 2004). It was suggested that in the Japanese eel, an uptake of H + triggers the initiation of sperm motility with the participation of a K + transport through a K + channel sensitive to 4-AP.…”

Section: Introductionmentioning

confidence: 99%

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Role of potassium and pH on the initiation of sperm motility in the European eel

Vílchez

Morini

Peñaranda

et al. 2017

Comparative Biochemistry and Physiology Part A: Molecular &

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The role of potassium from the seminal plasma and/or the activation media was examined by selectively removing K from this media, and by testing the use of K channel inhibitors and a K-ionophore. Sperm motility was measured using a CASA system, intracellular K and pH were measured by flow cytometry, and sperm head area was measured by ASMA: Automated Sperm Morphometry Analyses. Sperm motility was notably inhibited by the removal of K from the seminal plasma and by treatment with the K ionophore valinomycin. This therefore indicates that a reduction of K levels in the quiescent stage inhibits further motility. The normal decrease in sperm head area induced by seawater activation was altered by the removal of K from the seminal plasma, and an increase in the pH in the quiescent stage was also induced. Intracellular pH (pH) was quantitatively measured for the first time in European eel spermatozoa, being 7.2 in the quiescent stage and 7.1 post-activation. Intracellular and external pH levels influenced sperm motility both in the quiescent stage and at activation. The alkalinization of the pH (by NHCl) inhibited sperm motility activation, while acidification (by Na-acetate) did not have any effect. Our results indicate that a pH gradient between the sperm cell and the seminal plasma is necessary for sperm motility activation. The presence of the ion K in the seminal plasma (or in the extender medium) is necessary in order to maintain sperm volume, intracellular pH and sperm motility.

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Role of sodium bicarbonate on the initiation of sperm motility in the Japanese eel

Cited by 19 publications

References 26 publications

Effect of Sperm Cryopreservation on the European Eel Sperm Viability and Spermatozoa Morphology

Effect of Sperm Cryopreservation on the European Eel Sperm Viability and Spermatozoa Morphology

Effect of different methods for the induction of spermiation on semen quality in European eel

Role of potassium and pH on the initiation of sperm motility in the European eel

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