Inhibitory effect of magnolol on tumour metastasis in mice

Abstract

It has previously been reported that magnolol, a phenolic compound isolated from Magnolia obovata, inhibited tumour cell invasion in vitro. The purpose of this study was to investigate the antimetastatic effect of magnolol on tumour metastasis in vivo with experimental and spontaneous metastasis models and to clarify the mechanism. The antimetastatic effects of magnolol were evaluated by an experimental liver and spleen metastasis model using L5178Y-ML25 lymphoma, or an experimental and spontaneous lung metastasis model using B16-BL6 melanoma. Intraperitoneal (i.p.) administration of 2 or 10 mg/kg of magnolol significantly suppressed liver and spleen metastasis or lung metastasis. As for the spontaneous lung metastasis model using B16-BL6 melanoma, multiple i.p. administrations of 10 mg/kg of magnolol after and before tumour inoculation significantly suppressed lung metastasis and primary tumour growth. In addition, magnolol significantly inhibited B16-BL6 cell invasion of the reconstituted basement membrane (Matrigel, MG) without affecting cell growth. These data from the in vivo experiments suggest that magnolol possesses strong antimetastatic ability and that it may be a lead compound for drug development. The antimetastatic action of magnolol is considered to be due to its ability to inhibit tumour cell invasion.

Magnolol-Induced Apoptosis in HCT-116 Colon Cancer Cells Is Associated with the AMP-Activated Protein Kinase Signaling Pathway

“…19) The antimetastatic effects of magnolol in an experimental liver and spleen metastasis model using L5178YML25 lymphoma and in an experimental and spontaneous lung metastasis model of B16-BL6 melanoma, have been evaluated. 46) In this study, we determined the antimetastatic effect of magnolol via AMPK activation using wound migration and invasion assays. Our findings demonstrated that magnolol inhibited migration distance that was reversed by pretreatment with compound C (Fig.…”

Magnolol Suppresses MetastasisviaInhibition of Invasion, Migration, and Matrix Metalloproteinase-2/-9 Activities in PC-3 Human Prostate Carcinoma Cells

“…40) In an animal model, Ikeda et al 41) evaluated the anti-metastatic effects of magnolol in an experimental liver and spleen metastasis model using L5178Y-ML25 lymphoma, and in an experimental and spontaneous lung metastasis model using B16-BL6 melanoma. Magnolol significantly inhibited B16-BL6 cell invasion of the reconstituted basement membrane (Matrigel) without affecting cell growth.…”

Anti-diarrhea and anti-oxidant properties of Magnolol

“…Recent investigations have shown that magnolol possesses a wide range of physiological activities such as anti-tumor [4], anti-microbia [5], anti-clastogenic [6] and anti-platelet [7], .Moreover, while Cortex Magnoliae Officinalis has been used to treat diarrhea in traditional Chinese medicine, its anti-diarrhea active components are not defined and its mechanism of action is still not clear until now. Although some researchers have investigated the antioxidant activity of magnolol extracted from Cortex Magnoliae Officinalis [8], few of those studies were done in vivo.…”