Inhibitory effect of <i>Spirogyra</i>
 spp. algal extracts against herpes simplex virus type 1 and 2 infection

Deethae, Aomhatai; Peerapornpisal, Yuwadee; Pekkoh, Jeeraporn; Sangthong, Padchanee; Tragoolpua, Yingmanee

doi:10.1111/jam.13729

Cited by 19 publications

(9 citation statements)

References 36 publications

(54 reference statements)

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“…C10 and C11 reduced significantly the areas of formed viral plaques when compared to viral controls, and these results could be a consequence of the inhibition of viral release. Several studies have demonstrated the anti-herpes activity of natural products, some of which interfere with early steps [41,42,[56][57][58][59][60][61][62][63][64], while a few affect the last steps of HSV replication [65][66][67][68], as was demonstrated for C10 and C11. For this reason, both cardenolides could be used in combination with other natural products to ensure that different stages of HSV replication would be inhibited, for example with transresveratrol, for which significant anti-HSV activity has also been described [69].…”

Section: Discussionmentioning

confidence: 82%

Elucidation of the mechanism of anti-herpes action of two novel semisynthetic cardenolide derivatives

et al. 2020

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Human herpesviruses are among the most prevalent pathogens worldwide and have become an important public health issue. Recurrent infections and the emergence of resistant viral strains reinforce the need of searching new drugs to treat herpes virus infections. Cardiac glycosides are used clinically to treat cardiovascular disturbances, such as congestive heart failure and atrial arrhythmias. In recent years, they have sparked new interest in their potential anti-herpes action. It has been previously reported by our research group that two new semisynthetic cardenolides, namely C10 (3β-[(N-(2-hydroxyethyl)aminoacetyl] amino-3-deoxydigitoxigenin) and C11 (3β-(hydroxyacetyl)amino-3-deoxydigitoxigenin), exhibited potential anti-HSV-1 and anti-HSV-2 with selectivity index values > 1,000, comparable with those of acyclovir. This work reports the mechanism investigation of anti-herpes action of these derivatives. The results demonstrated that C10 and C11 interfere with the intermediate and final steps of HSV replication, but not with the early stages, since they completely abolished the expression of the UL42 (β) and gD (γ) proteins and partially reduced that of ICP27 (α). Additionally, they were not virucidal and had no prophylactic effects. Both compounds inhibited HSV replication at nanomolar concentrations, but cardenolide C10 was more active than C11 and can be considered as an anti-herpes drug candidate including against acyclovir-resistant HSV-1 strains. AbbreviationsACV acyclovir CC 50 50% cytotoxic concentration CMC carboxymethylcellulose DEX-S dextran sulfate FBS fetal bovine serum HIV human immunodeficiency virus HSV-1 herpes simplex virus type 1 HSV-2 herpes simplex virus type 2 HPV human papillomavirus IC 50 concentration that inhibited 50% of viral replication MEM Eagle's minimum essential medium MOI multiplicity of infection PBS phosphate-buffered saline PFU plaque-forming units SI selectivity index

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Section: Discussionmentioning

confidence: 82%

Elucidation of the mechanism of anti-herpes action of two novel semisynthetic cardenolide derivatives

et al. 2020

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“…had highly significant inhibition of the viral infection on HSV-1 and HSV-2, respectively, when treated during the viral infection phase of Vero cells. The alkaloids, essential oils and terpenoids present in the freshwater macroalgae were regarded as the main active compounds responsible for anti-viral activity (Deethae et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial effects of the organic crude extracts of freshwater algae of Sulaymaniyah, Kurdistan Region, Iraq

Thomas¹,

Ghafour²,

Diyya³

et al. 2021

J. Med. Plants Res.

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In the recent past, the importance of freshwater algae has grown enormously due to their antibiotic activity against certain species of bacteria known for several disease states like endocarditis, external otitis, skin rash, etc. Also, there is a growing concern among the immunocompromised individuals that they may be susceptible to antibiotics and anti-fungal resistant infections resulting in increased fatality rates. Hence, in this investigation, extraction of potentially bioactive compounds from natural resources like freshwater algae was performed along with the evaluation of the antimicrobial activities of these extracts against some opportunistic bacteria like Staphylococcus aureus, Escherichia coli, Staphylococcus xylosus and Pseudomonas aeruginosa using well diffusion method. The data showed a statistically significant (P < 0.05) antibacterial activity of the organic extracts (0.1 g/ml) obtained from freshwater algae against multi-drug resistant S. aureus and S. xylosus strains as compared to the control. Our data reinforce the importance of bioactive compounds from fresh algae as potential antimicrobial agents, and they could act as an alternative to conventional antibiotics.

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“…Lastly, Deethae et al 158 created aqueous, ethanolic, and methanolic extracts from the freshwater green algae Spirogyra and investigated antiviral activity against HSV-1 (strain F) and HSV-2 (strain G). Firstly, effects were when extracts were added to cells before, during, and after viral attachment.…”

Section: Asiamentioning

confidence: 99%

Review of Whole Plant Extracts With Activity Against Herpes Simplex Viruses In Vitro and In Vivo

Garber

Barnard

Pickrell

2021

J Evid Based Complementary Altern Med

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Herpes simplex viruses, HSV-1 and HSV-2, are highly contagious and cause lifelong, latent infections with recurrent outbreaks of oral and/or genital lesions. No cure exists for HSV-1 or HSV-2 infections, but antiviral medications are commonly used to prevent and treat outbreaks. Resistance to antivirals has begun to emerge, placing an importance on finding new and effective therapies for prophylaxis and treatment of HSV outbreaks. Botanicals may be effective HSV therapies as the constituents they contain act through a variety of mechanisms, potentially making the development of antiviral resistance more challenging. A wide variety of plants from different regions in the world have been studied for antiviral activity against HSV-1 and/or HSV-2 and showed efficacy of varying degrees. The purpose of this review is to summarize research conducted on whole plant extracts against HSV-1 and/or HSV-2 in vitro and in vivo. The majority of the research reviewed was conducted in vitro using animal cell lines, and some studies used an animal model design. Also summarized are a limited number of human trials conducted using botanical therapies on HSV lesions.

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Inhibitory effect of Spirogyra spp. algal extracts against herpes simplex virus type 1 and 2 infection

Abstract: Anti-HSV activity of extract of freshwater green macroalga Spirogyra spp. in Thailand was demonstrated. Therefore, anti-HSV product containing the Spirogyra spp. extract should be developed for treatment of HSV infection.

Cited by 19 publications

References 36 publications

Elucidation of the mechanism of anti-herpes action of two novel semisynthetic cardenolide derivatives

Elucidation of the mechanism of anti-herpes action of two novel semisynthetic cardenolide derivatives

Antibacterial effects of the organic crude extracts of freshwater algae of Sulaymaniyah, Kurdistan Region, Iraq

Review of Whole Plant Extracts With Activity Against Herpes Simplex Viruses In Vitro and In Vivo

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